Gene targets for improved enzyme production in fungi
Abstract
Fungi that are genetically inactivated for the mstC gene (or a homolog thereof) are provided, which can also be genetically modified to increase production of heterologous proteins from a glucoamylase promoter. Methods of using these fungi, for example to degrade a biomass, are also provided.
- Inventors:
- Issue Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 2222045
- Patent Number(s):
- 11718854
- Application Number:
- 17/161,502
- Assignee:
- Battelle Memorial Institute (Richland, WA); National Technology & Engineering Solutions of Sandia, LLC (Albuquerque, NM)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- AC02-05CH11231; AC05-76RL01830
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 01/28/2021
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Baker, Scott E., Magnuson, Jon K., Reilly, Morgann C., Kim, Joonhoon, Gladden, John, and Lynn, Jed J. Gene targets for improved enzyme production in fungi. United States: N. p., 2023.
Web.
Baker, Scott E., Magnuson, Jon K., Reilly, Morgann C., Kim, Joonhoon, Gladden, John, & Lynn, Jed J. Gene targets for improved enzyme production in fungi. United States.
Baker, Scott E., Magnuson, Jon K., Reilly, Morgann C., Kim, Joonhoon, Gladden, John, and Lynn, Jed J. Tue .
"Gene targets for improved enzyme production in fungi". United States. https://www.osti.gov/servlets/purl/2222045.
@article{osti_2222045,
title = {Gene targets for improved enzyme production in fungi},
author = {Baker, Scott E. and Magnuson, Jon K. and Reilly, Morgann C. and Kim, Joonhoon and Gladden, John and Lynn, Jed J.},
abstractNote = {Fungi that are genetically inactivated for the mstC gene (or a homolog thereof) are provided, which can also be genetically modified to increase production of heterologous proteins from a glucoamylase promoter. Methods of using these fungi, for example to degrade a biomass, are also provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2023},
month = {8}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Aspergillus niger membrane-associated proteome analysis for the identification of glucose transporters
journal, September 2015
- Sloothaak, J.; Odoni, D. I.; de Graaff, L. H.
- Biotechnology for Biofuels, Vol. 8, Issue 1
Regulation of the glaA gene of Aspergillus niger
journal, January 1990
- Fowler, Timothy; Berka, Randy M.; Ward, Michael
- Current Genetics, Vol. 18, Issue 6
Two glucose transporters in Saccharomyces cerevisiae are glucose sensors that generate a signal for induction of gene expression.
journal, October 1996
- Ozcan, S.; Dover, J.; Rosenwald, A. G.
- Proceedings of the National Academy of Sciences, Vol. 93, Issue 22
Identification of the mstE Gene Encoding a Glucose-inducible, Low Affinity Glucose Transporter in Aspergillus nidulans
journal, January 2006
- Forment, Josep V.; Flipphi, Michel; Ramón, Daniel
- Journal of Biological Chemistry, Vol. 281, Issue 13
DELLY: structural variant discovery by integrated paired-end and split-read analysis
journal, September 2012
- Rausch, T.; Zichner, T.; Schlattl, A.
- Bioinformatics, Vol. 28, Issue 18
A Thermophilic Ionic Liquid-Tolerant Cellulase Cocktail for the Production of Cellulosic Biofuels
journal, May 2012
- Park, Joshua I.; Steen, Eric J.; Burd, Helcio
- PLoS ONE, Vol. 7, Issue 5, Article No. e37010
WILD-TYPE AND MUTANT STOCKS OF ASPERGILLUS NIDULANS
journal, July 1965
- Barratt, R. W.; Johnson, G. B.; Ogata, W. N.
- Genetics, Vol. 52, Issue 1
Toward better understanding of artifacts in variant calling from high-coverage samples
journal, June 2014
- Li, Heng
- Bioinformatics, Vol. 30, Issue 20
High-Affinity Glucose Transport in Aspergillus nidulans Is Mediated by the Products of Two Related but Differentially Expressed Genes
journal, April 2014
- Forment, Josep V.; Flipphi, Michel; Ventura, Luisa
- PLoS ONE, Vol. 9, Issue 4
Regulation of β-glucosidase biosynthesis in Aspergillus nidulans
journal, January 1996
- Lee, J.
- FEMS Microbiology Letters, Vol. 135, Issue 1
Errors in genome annotation
journal, April 1999
- Brenner, Steven E.
- Trends in Genetics, Vol. 15, Issue 4
Toward a Systems Approach to Understanding Plant Cell Walls
journal, December 2004
- Somerville, C.
- Science, Vol. 306, Issue 5705
Ionic Liquid-Tolerant Cellulase Enzymes
patent-application,
- Gladden, John; Park, Joshua; Singer, Steven
- US Patent Application 14/612201; 20150218543
Characterization of a polyketide synthase in Aspergillus niger whose product is a precursor for both dihydroxynaphthalene (DHN) melanin and naphtho-γ-pyrone
journal, April 2011
- Chiang, Yi-Ming; Meyer, Kristen M.; Praseuth, Michael
- Fungal Genetics and Biology, Vol. 48, Issue 4
Glucose uptake and growth of glucose-limited chemostat cultures of Aspergillus niger and a disruptant lacking MstA, a high-affinity glucose transporter
journal, June 2007
- Jorgensen, T. R.; vanKuyk, P. A.; Poulsen, B. R.
- Microbiology, Vol. 153, Issue 6
Ypt/Rab GTPases: Principles learned from yeast
journal, November 2014
- Lipatova, Zhanna; Hain, Adelaide U.; Nazarko, Volodymyr Y.
- Critical Reviews in Biochemistry and Molecular Biology, Vol. 50, Issue 3
A statistical framework for SNP calling, mutation discovery, association mapping and population genetical parameter estimation from sequencing data
journal, September 2011
- Li, H.
- Bioinformatics, Vol. 27, Issue 21
Glycoside Hydrolase Activities of Thermophilic Bacterial Consortia Adapted to Switchgrass
journal, July 2011
- Gladden, John M.; Allgaier, Martin; Miller, Christopher S.
- Applied and Environmental Microbiology, Vol. 77, Issue 16, p. 5804-5812
The Genome Analysis Toolkit: A MapReduce framework for analyzing next-generation DNA sequencing data
journal, July 2010
- McKenna, A.; Hanna, M.; Banks, E.
- Genome Research, Vol. 20, Issue 9
Transceptors as a functional link of transporters and receptors
journal, March 2017
- Diallinas, George
- Microbial Cell, Vol. 4, Issue 3
Pyrithiamine Resistance Gene ( ptrA ) of Aspergillus oryzae : Cloning, Characterization and Application as a Dominant Selectable Marker for Transformation
journal, January 2000
- Kubodera, Takafumi; Yamashita, Nobuo; Nishimura, Akira
- Bioscience, Biotechnology, and Biochemistry, Vol. 64, Issue 7
Synthesis of glucose esters from cellulose in ionic liquids
journal, May 2012
- Ignatyev, Igor A.; Doorslaer, Charlie Van; Mertens, Pascal G. N.
- Holzforschung, Vol. 66, Issue 4
LUMPY: a probabilistic framework for structural variant discovery
journal, January 2014
- Layer, Ryan M.; Chiang, Colby; Quinlan, Aaron R.
- Genome Biology, Vol. 15, Issue 6
Pindel: a pattern growth approach to detect break points of large deletions and medium sized insertions from paired-end short reads
journal, June 2009
- Ye, K.; Schulz, M. H.; Long, Q.
- Bioinformatics, Vol. 25, Issue 21
Current Pretreatment Technologies for the Development of Cellulosic Ethanol and Biorefineries
journal, September 2015
- Silveira, Marcos Henrique Luciano; Morais, Ana Rita C.; da Costa Lopes, Andre M.
- ChemSusChem, Vol. 8, Issue 20
The Cell Factory Aspergillus Enters the Big Data Era: Opportunities and Challenges for Optimising Product Formation
book, January 2015
- Meyer, Vera; Fiedler, Markus; Nitsche, Benjamin
- Advances in Biochemical Engineering/Biotechnology
Discovery and characterization of ionic liquid-tolerant thermophilic cellulases from a switchgrass-adapted microbial community
journal, January 2014
- Gladden, John M.; Park, Joshua I.; Bergmann, Jessica
- Biotechnology for Biofuels, Vol. 7, Issue 1
VariantAnnotation: a Bioconductor package for exploration and annotation of genetic variants
journal, March 2014
- Obenchain, V.; Lawrence, M.; Carey, V.
- Bioinformatics, Vol. 30, Issue 14
Gene Targets for Improved Enzyme Production in Fungi
patent-application, April 2019
- Baker, Scott E.; Magnuson, Jon K.; Reilly, Morgann C.
- US Patent Application 16/163509; 20190112611
The Sequence Alignment/Map format and SAMtools
journal, June 2009
- Li, H.; Handsaker, B.; Wysoker, A.
- Bioinformatics, Vol. 25, Issue 16
Expression Profile of Amylolytic Genes in Aspergillus nidulans
journal, October 2006
- Nakamura, Takashi; Maeda, Yuki; Tanoue, Nami
- Bioscience, Biotechnology, and Biochemistry, Vol. 70, Issue 10
Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts
journal, November 2016
- Yu, Chaowei; Simmons, Blake A.; Singer, Steven W.
- Applied Microbiology and Biotechnology, Vol. 100, Issue 24
Regulation of Plant Biomass Utilization in Aspergillus
book, January 2014
- Kowalczyk, Joanna E.; Benoit, Isabelle; de Vries, Ronald P.
- Advances in Applied Microbiology
Carbon repression in aspergilli
journal, June 1997
- Ruijter, George J. G.; Visser, Jaap
- FEMS Microbiology Letters, Vol. 151, Issue 2
Transcription Factor CCG-8 as a New Regulator in the Adaptation to Antifungal Azole Stress
journal, March 2014
- Sun, Xianyun; Wang, Kangji; Yu, Xinxu
- Antimicrobial Agents and Chemotherapy, Vol. 58, Issue 3
In-depth analysis of the Aspergillus niger glucoamylase (glaA) promoter performance using high-throughput screening and controlled bioreactor cultivation techniques
journal, June 2008
- Ganzlin, M.; Rinas, U.
- Journal of Biotechnology, Vol. 135, Issue 3
A broader role for AmyR in Aspergillus niger: regulation of the utilisation of d-glucose or d-galactose containing oligo- and polysaccharides
journal, August 2011
- vanKuyk, Patricia A.; Benen, Jaques A. E.; Wösten, Han A. B.
- Applied Microbiology and Biotechnology, Vol. 93, Issue 1
The low affinity glucose transporter HxtB is also involved in glucose signalling and metabolism in Aspergillus nidulans
journal, March 2017
- dos Reis, Thaila Fernanda; Nitsche, Benjamin M.; de Lima, Pollyne Borborema Almeida
- Scientific Reports, Vol. 7, Issue 1
Expression of naturally ionic liquid-tolerant thermophilic cellulases in Aspergillus niger
journal, December 2017
- Amaike Campen, Saori; Lynn, Jed; Sibert, Stephanie J.
- PLOS ONE, Vol. 12, Issue 12
On the safety of Aspergillus niger - a review
journal, January 2002
- Schuster, E.; Dunn-Coleman, N.; Frisvad, J.
- Applied Microbiology and Biotechnology, Vol. 59, Issue 4-5, p. 426-435
Mini-review: In vitro Metabolic Engineering for Biomanufacturing of High-value Products
journal, January 2017
- Guo, Weihua; Sheng, Jiayuan; Feng, Xueyang
- Computational and Structural Biotechnology Journal, Vol. 15
Forward genetics screen coupled with whole-genome resequencing identifies novel gene targets for improving heterologous enzyme production in Aspergillus niger
journal, January 2018
- Reilly, Morgann C.; Kim, Joonhoon; Lynn, Jed
- Applied Microbiology and Biotechnology, Vol. 102, Issue 4
BreakDancer: an algorithm for high-resolution mapping of genomic structural variation
journal, August 2009
- Chen, Ken; Wallis, John W.; McLellan, Michael D.
- Nature Methods, Vol. 6, Issue 9
Deletion of glucose oxidase changes the pattern of organic acid production in Aspergillus carbonarius
journal, August 2014
- Yang, Lei; Lübeck, Mette; Lübeck, Peter S.
- AMB Express, Vol. 4, Issue 1