Title: Yeast cells having disrupted pathway from dihydroxyacetone phosphate to glycerol

Abstract

Yeast cells are genetically modified to disrupt a native metabolic pathway from dihydroxyacetone to glycerol. In certain aspects, the yeast cell is of the genera Kluyveromyces, Candida or Issatchenkia. In other aspects, the yeast cell is capable of producing at least one organic acid, such as lactate. The yeast cells produce significantly less glycerol than the wild-type strains, and usually produce greater yields of desired fermentation products. Yeast cells of the invention often grow well when cultivated, despite their curtailed glycerol production.

Inventors:

Dundon, Catherine Asleson; Suominen, Pirkko; Aristidou, Aristos; Rush, Brian J.; Koivuranta, Kari; Hause, Benjamin Matthew; McMullin, Thomas William; Roberg-Perez, Kevin

Issue Date:

2023-07-04

Research Org.:

Cargill, Inc., Wayzata, MN (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

2221936

Patent Number(s):

11691817

Application Number:

17/154,190

Assignee:

Cargill, Incorporated (Wayzata, MN)

Patent Classifications (CPCs):

B - PERFORMING OPERATIONS B65 - CONVEYING B65G - TRANSPORT OR STORAGE DEVICES, e.g. CONVEYORS FOR LOADING OR TIPPING, SHOP CONVEYOR SYSTEMS OR PNEUMATIC TUBE CONVEYORS

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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B - PERFORMING OPERATIONS B65 - CONVEYING B65G - TRANSPORT OR STORAGE DEVICES, e.g. CONVEYORS FOR LOADING OR TIPPING, SHOP CONVEYOR SYSTEMS OR PNEUMATIC TUBE CONVEYORS

B65G15/12 - with two or more endless belts
B65G17/12 - comprising a series of individual load-carriers fixed, or normally fixed, relative to traction element

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/52 - Genes encoding for enzymes or proenzymes

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P7/06 - Ethanol, i.e. non-beverage
C12P7/40 - containing a carboxyl group {including Peroxycarboxylic acids
C12P7/56 - Lactic acid

E - FIXED CONSTRUCTIONS E01 - CONSTRUCTION OF ROADS, RAILWAYS, OR BRIDGES E01B - PERMANENT WAY

E01B29/32 - Installing or removing track components, not covered by the preceding groups, e.g. sole-plates, rail anchors

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION

Y02E50/10 - Biofuels

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

Y10T137/85986 - Pumped fluid control

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DOE Contract Number:  

FC36-03GO13145

Resource Type:

Patent

Resource Relation:

Patent File Date: 01/21/2021

Country of Publication:

United States

Language:

English