Methods for making polypeptides including d-amino acids
Abstract
A method of making a polypeptide including one or more D-amino acids is provided. The method includes combining a ribosome with protein translation factors including (1) a template encoding the polypeptide, wherein the template encoding the polypeptide includes one or more codons which have been recoded to accept a tRNA attached to a D-amino acid, (2) a plurality of L-amino acids and a plurality of corresponding tRNAs, (3) a plurality of D-amino acids and their corresponding aminoacyl tRNA synthetase or a plurality of tRNAs ligated with a D-amino acid, and (4) elongation factor P in a concentration of 2 to 20 micromolar, wherein translation of the template encoding the polypeptide occurs to produce the polypeptide including one or more D-amino acids.
- Inventors:
- Issue Date:
- Research Org.:
- Harvard Univ., Cambridge, MA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1998528
- Patent Number(s):
- 11685942
- Application Number:
- 16/081,989
- Assignee:
- President & Fellows of Harvard College (Cambridge, MA)
- DOE Contract Number:
- FG02-02ER63445
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 03/03/2017
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Church, George M., and Huang, Po-Yi. Methods for making polypeptides including d-amino acids. United States: N. p., 2023.
Web.
Church, George M., & Huang, Po-Yi. Methods for making polypeptides including d-amino acids. United States.
Church, George M., and Huang, Po-Yi. Tue .
"Methods for making polypeptides including d-amino acids". United States. https://www.osti.gov/servlets/purl/1998528.
@article{osti_1998528,
title = {Methods for making polypeptides including d-amino acids},
author = {Church, George M. and Huang, Po-Yi},
abstractNote = {A method of making a polypeptide including one or more D-amino acids is provided. The method includes combining a ribosome with protein translation factors including (1) a template encoding the polypeptide, wherein the template encoding the polypeptide includes one or more codons which have been recoded to accept a tRNA attached to a D-amino acid, (2) a plurality of L-amino acids and a plurality of corresponding tRNAs, (3) a plurality of D-amino acids and their corresponding aminoacyl tRNA synthetase or a plurality of tRNAs ligated with a D-amino acid, and (4) elongation factor P in a concentration of 2 to 20 micromolar, wherein translation of the template encoding the polypeptide occurs to produce the polypeptide including one or more D-amino acids.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2023},
month = {6}
}
Works referenced in this record:
Enhanced d -Amino Acid Incorporation into Protein by Modified Ribosomes
journal, June 2003
- Dedkova, Larisa M.; Fahmi, Nour Eddine; Golovine, Serguei Y.
- Journal of the American Chemical Society, Vol. 125, Issue 22
Outwitting EF-Tu and the ribosome: translation with d-amino acids
journal, May 2015
- Achenbach, John; Jahnz, Michael; Bethge, Lucas
- Nucleic Acids Research, Vol. 43, Issue 12
EF-P Is Essential for Rapid Synthesis of Proteins Containing Consecutive Proline Residues
journal, December 2012
- Doerfel, Lili K.; Wohlgemuth, Ingo; Kothe, Christina
- Science, Vol. 339, Issue 6115