Detection of cholinesterase inhibition with microfluidic devices and systems thereof

Koh, Chung-Yan; Meagher, Robert; Eckles, Tyler Phillips

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Title: Detection of cholinesterase inhibition with microfluidic devices and systems thereof

Abstract

The present invention relates to methods of conducting cholinesterase inhibition assays. In one instance, the assays can be configured to determine the presence of inactivated and activated cholinesterases. Also described herein are microfluidic devices and systems for conducting such assays.

Inventors:: Koh, Chung-Yan; Meagher, Robert; Eckles, Tyler Phillips

Issue Date:: 2023-06-20

Research Org.:: Sandia National Lab. (SNL-CA), Livermore, CA (United States)

Sponsoring Org.:: USDOE National Nuclear Security Administration (NNSA)

OSTI Identifier:: 1998508

Patent Number(s):: 11680946

Application Number:: 17/143,363

Assignee:: National Technology & Engineering Solutions of Sandia, LLC (Albuquerque, NM)

DOE Contract Number:: NA0003525

Resource Type:: Patent

Resource Relation:: Patent File Date: 01/07/2021

Country of Publication:: United States

Language:: English

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                    Koh, Chung-Yan, Meagher, Robert, and Eckles, Tyler Phillips. Detection of cholinesterase inhibition with microfluidic devices and systems thereof.  United States: N. p., 2023. 
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                    Koh, Chung-Yan, Meagher, Robert, & Eckles, Tyler Phillips. Detection of cholinesterase inhibition with microfluidic devices and systems thereof.  United States.

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                    Koh, Chung-Yan, Meagher, Robert, and Eckles, Tyler Phillips. Tue .  
        "Detection of cholinesterase inhibition with microfluidic devices and systems thereof".  United States.  https://www.osti.gov/servlets/purl/1998508.

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@article{osti_1998508,

  title        = {Detection of cholinesterase inhibition with microfluidic devices and systems thereof},

  author       = {Koh, Chung-Yan and Meagher, Robert and Eckles, Tyler Phillips},

  abstractNote = {The present invention relates to methods of conducting cholinesterase inhibition assays. In one instance, the assays can be configured to determine the presence of inactivated and activated cholinesterases. Also described herein are microfluidic devices and systems for conducting such assays.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2023},

  month        = {6}

}

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Title: Detection of cholinesterase inhibition with microfluidic devices and systems thereof

Abstract

Citation Formats

System and method for detecting components of a mixture including a valving scheme for competition assays patent, July 2017

Thiol–ene click reaction-induced fluorescence enhancement by altering the radiative rate for assaying butyrylcholinesterase activity journal, January 2019

Advantages of the WRAIR whole blood cholinesterase assay: Comparative analysis to the micro-Ellman, Test-mate ChE™, and Michel (ΔpH) assays journal, September 2008

Comparison of the Binding of Reversible Inhibitors to Human Butyrylcholinesterase and Acetylcholinesterase: A Crystallographic, Kinetic and Calorimetric Study journal, November 2017

Microfluidic devices, systems, and methods for quantifying particles using centrifugal force patent, November 2015

Magnetic Electrochemical Sensing Platform for Biomonitoring of Exposure to Organophosphorus Pesticides and Nerve Agents Based on Simultaneous Measurement of Total Enzyme Amount and Enzyme Activity journal, April 2011

Detection of active and latent infections with microfluidic devices and systems thereof patent, September 2020

Devices, systems, and methods for detecting nucleic acids using sedimentation patent, August 2019

Field Verification of Test-Mate ChE journal, April 2003

Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation patent, February 2015

Construction of an Acetylcholinesterase Sensor Based on Synthesized Paramagnetic Nanoparticles, a Simple Tool for Neurotoxic Compounds Assay journal, March 2017

In situ induced metal-enhanced fluorescence: A new strategy for biosensing the total acetylcholinesterase activity in sub-microliter human whole blood journal, June 2015

Devices, systems, and methods for detecting nucleic acids using sedimentation patent-application, March 2018

Luminescent silica nanoparticles for sensing acetylcholinesterase-catalyzed hydrolysis of acetylcholine journal, March 2016

Synthesis and biological evaluation of 8-hydroxy-2,7-naphthyridin-2-ium salts as novel inhibitors of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) journal, January 2017

Foldable paper-based analytical device for the detection of an acetylcholinesterase inhibitor using an angle-based readout journal, November 2018

Detection of the Degree of Exposure to Chemical Warfare Nerve Agents and Organophosphate Pesticides with Lateral Flow Assays patent-application, December 2016

Evaluation of the Test-mate ChE (Cholinesterase) Field Kit in Acute Organophosphorus Poisoning journal, December 2011

Application of gold–silver nanocluster based fluorescent sensors for determination of acetylcholinesterase activity and its inhibitor journal, June 2018

Quantitative detection of pathogens in centrifugal microfluidic disks patent, February 2018

Comparison of 5 monoclonal antibodies for immunopurification of human butyrylcholinesterase on Dynabeads: KD values, binding pairs, and amino acid sequences journal, October 2015

Toxin activity assays, devices, methods and systems therefor patent, April 2016

On-site analysis of acetylcholinesterase and butyrylcholinesterase activity with the ChE check mobile test kit—Determination of reference values and their relevance for diagnosis of exposure to organophosphorus compounds journal, May 2016

A sensitive biosensor for the fluorescence detection of the acetylcholinesterase reaction system based on carbon dots journal, January 2015

Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation patent, April 2016

A novel fluorogenic probe for the investigation of free thiols: Application to kinetic measurements of acetylcholinesterase activity journal, February 2016

Cholinesterase measurements with an automated kit journal, August 2002

Immunopurification of Acetylcholinesterase from Red Blood Cells for Detection of Nerve Agent Exposure journal, September 2017

Evaluation of salivary acetylcholinesterase and pseudocholinesterase in patients with Alzheimer's disease: A case–control study journal, December 2018

Devices, systems, and methods for detecting nucleic acids using sedimentation patent, October 2017

Devices, Systems, and Methods for Conducting Assays with Improved Sensitivity Using Sedimentation patent-application, June 2014

Protein Surface Structural Recognition in Inactive Areas: A New Immobilization Strategy for Acetylcholinesterase journal, April 2018

Systems, devices, and methods for agglutination assays using sedimentation patent, January 2016

CD-like centrifugal microfluidic device for organophosphorus pesticides (OPP) sensing conference, August 2017

A ratiometric fluorescence assay for acetylcholinesterase activity and inhibitor screening based on supramolecular assembly induced monomer–excimer emission transition of a perylene probe journal, January 2018

Method and apparatus for purifying nucleic acids and performing polymerase chain reaction assays using an immiscible fluid patent, October 2017

An on–off–on gold nanocluster-based fluorescent probe for sensitive detection of organophosphorus pesticides journal, January 2017

Non-contact temperature control system for microfluidic devices patent, September 2019

Quantitative detection of pathogens in centrifugal microfluidic disks patent-application, February 2018

Rapid quantification of two chemical nerve agent metabolites in serum journal, April 2019

System and method for detecting components of a mixture including a valving scheme for competition assays patent, September 2017

History and New Developments of Assays for Cholinesterase Activity and Inhibition journal, July 2010

Systems, devices, and methods for agglutination assays using sedimentation patent, July 2018

Devices, systems, and methods for conducting sandwich assays using sedimentation patent, February 2015

Integrated LAMP and immunoassay platform for diarrheal disease detection journal, November 2018

Simple and sensitive fluorescence assay for acetylcholinesterase activity detection and inhibitor screening based on glutathione-capped gold nanoclusters journal, December 2017

An inhibitory monoclonal antibody to human acetylcholinesterases journal, March 1987

Endpoint detection of amplified nucleic acids patent, July 2020

Feasibility of Portable Fingerstick Cholinesterase Testing in Adolescents in South Texas journal, January 2019

Microfluidic Assays of Acetylcholinesterase Inhibitors journal, October 1999

System and method for detecting components of a mixture including tooth elements for alignment patent, November 2016

On-substrate Enzymatic Reaction to Determine Acetylcholinesterase Activity in Whole Blood by Paper Spray Mass Spectrometry journal, October 2018

Integrated Lateral Flow Test Strip with Electrochemical Sensor for Quantification of Phosphorylated Cholinesterase: Biomarker of Exposure to Organophosphorus Agents journal, January 2012

A novel fluorescence based assay for the detection of organophosphorus pesticide exposed cholinesterase activity using 1-naphthyl acetate journal, May 2019

Method and apparatus for purifying nucleic acids and performing polymerase chain reaction assays using an immiscible fluid patent, March 2020

Detection of metabolites for controlled substances patent, April 2019

Microfluidic Devices, Systems, And Methods For Quantifying Particles Using Centrifugal Force patent-application, December 2015

System and method for detecting components of a mixture including a valving scheme for competition assays
patent, July 2017

Thiol–ene click reaction-induced fluorescence enhancement by altering the radiative rate for assaying butyrylcholinesterase activity
journal, January 2019

Advantages of the WRAIR whole blood cholinesterase assay: Comparative analysis to the micro-Ellman, Test-mate ChE™, and Michel (ΔpH) assays
journal, September 2008

Comparison of the Binding of Reversible Inhibitors to Human Butyrylcholinesterase and Acetylcholinesterase: A Crystallographic, Kinetic and Calorimetric Study
journal, November 2017

Microfluidic devices, systems, and methods for quantifying particles using centrifugal force
patent, November 2015

Magnetic Electrochemical Sensing Platform for Biomonitoring of Exposure to Organophosphorus Pesticides and Nerve Agents Based on Simultaneous Measurement of Total Enzyme Amount and Enzyme Activity
journal, April 2011

Detection of active and latent infections with microfluidic devices and systems thereof
patent, September 2020

Devices, systems, and methods for detecting nucleic acids using sedimentation
patent, August 2019

Field Verification of Test-Mate ChE
journal, April 2003

Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation
patent, February 2015

Construction of an Acetylcholinesterase Sensor Based on Synthesized Paramagnetic Nanoparticles, a Simple Tool for Neurotoxic Compounds Assay
journal, March 2017

In situ induced metal-enhanced fluorescence: A new strategy for biosensing the total acetylcholinesterase activity in sub-microliter human whole blood
journal, June 2015

Devices, systems, and methods for detecting nucleic acids using sedimentation
patent-application, March 2018

Luminescent silica nanoparticles for sensing acetylcholinesterase-catalyzed hydrolysis of acetylcholine
journal, March 2016

Synthesis and biological evaluation of 8-hydroxy-2,7-naphthyridin-2-ium salts as novel inhibitors of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE)
journal, January 2017

Foldable paper-based analytical device for the detection of an acetylcholinesterase inhibitor using an angle-based readout
journal, November 2018

Detection of the Degree of Exposure to Chemical Warfare Nerve Agents and Organophosphate Pesticides with Lateral Flow Assays
patent-application, December 2016

Evaluation of the Test-mate ChE (Cholinesterase) Field Kit in Acute Organophosphorus Poisoning
journal, December 2011

Application of gold–silver nanocluster based fluorescent sensors for determination of acetylcholinesterase activity and its inhibitor
journal, June 2018

Quantitative detection of pathogens in centrifugal microfluidic disks
patent, February 2018

Comparison of 5 monoclonal antibodies for immunopurification of human butyrylcholinesterase on Dynabeads: KD values, binding pairs, and amino acid sequences
journal, October 2015

Toxin activity assays, devices, methods and systems therefor
patent, April 2016

On-site analysis of acetylcholinesterase and butyrylcholinesterase activity with the ChE check mobile test kit—Determination of reference values and their relevance for diagnosis of exposure to organophosphorus compounds
journal, May 2016

A sensitive biosensor for the fluorescence detection of the acetylcholinesterase reaction system based on carbon dots
journal, January 2015

Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation
patent, April 2016

A novel fluorogenic probe for the investigation of free thiols: Application to kinetic measurements of acetylcholinesterase activity
journal, February 2016

Cholinesterase measurements with an automated kit
journal, August 2002

Immunopurification of Acetylcholinesterase from Red Blood Cells for Detection of Nerve Agent Exposure
journal, September 2017

Evaluation of salivary acetylcholinesterase and pseudocholinesterase in patients with Alzheimer's disease: A case–control study
journal, December 2018

Devices, systems, and methods for detecting nucleic acids using sedimentation
patent, October 2017

Devices, Systems, and Methods for Conducting Assays with Improved Sensitivity Using Sedimentation
patent-application, June 2014

Protein Surface Structural Recognition in Inactive Areas: A New Immobilization Strategy for Acetylcholinesterase
journal, April 2018

Systems, devices, and methods for agglutination assays using sedimentation
patent, January 2016

CD-like centrifugal microfluidic device for organophosphorus pesticides (OPP) sensing
conference, August 2017

A ratiometric fluorescence assay for acetylcholinesterase activity and inhibitor screening based on supramolecular assembly induced monomer–excimer emission transition of a perylene probe
journal, January 2018

Method and apparatus for purifying nucleic acids and performing polymerase chain reaction assays using an immiscible fluid
patent, October 2017

An on–off–on gold nanocluster-based fluorescent probe for sensitive detection of organophosphorus pesticides
journal, January 2017

Non-contact temperature control system for microfluidic devices
patent, September 2019

Quantitative detection of pathogens in centrifugal microfluidic disks
patent-application, February 2018

Rapid quantification of two chemical nerve agent metabolites in serum
journal, April 2019

System and method for detecting components of a mixture including a valving scheme for competition assays
patent, September 2017

History and New Developments of Assays for Cholinesterase Activity and Inhibition
journal, July 2010

Systems, devices, and methods for agglutination assays using sedimentation
patent, July 2018

Devices, systems, and methods for conducting sandwich assays using sedimentation
patent, February 2015

Integrated LAMP and immunoassay platform for diarrheal disease detection
journal, November 2018

Simple and sensitive fluorescence assay for acetylcholinesterase activity detection and inhibitor screening based on glutathione-capped gold nanoclusters
journal, December 2017

An inhibitory monoclonal antibody to human acetylcholinesterases
journal, March 1987

Endpoint detection of amplified nucleic acids
patent, July 2020

Feasibility of Portable Fingerstick Cholinesterase Testing in Adolescents in South Texas
journal, January 2019

Microfluidic Assays of Acetylcholinesterase Inhibitors
journal, October 1999

System and method for detecting components of a mixture including tooth elements for alignment
patent, November 2016

On-substrate Enzymatic Reaction to Determine Acetylcholinesterase Activity in Whole Blood by Paper Spray Mass Spectrometry
journal, October 2018

Integrated Lateral Flow Test Strip with Electrochemical Sensor for Quantification of Phosphorylated Cholinesterase: Biomarker of Exposure to Organophosphorus Agents
journal, January 2012

A novel fluorescence based assay for the detection of organophosphorus pesticide exposed cholinesterase activity using 1-naphthyl acetate
journal, May 2019

Method and apparatus for purifying nucleic acids and performing polymerase chain reaction assays using an immiscible fluid
patent, March 2020

Detection of metabolites for controlled substances
patent, April 2019

Microfluidic Devices, Systems, And Methods For Quantifying Particles Using Centrifugal Force
patent-application, December 2015