Title: Host cells and methods for producing isopentenol from mevalonate

Abstract

The present invention provides for a genetically modified host cell capable of producing isopentenol and/or 3-methyl-3-butenol, comprising (a) an increased expression of phosphomevalonate decarboxylase (PMD) (b) an increased expression of a phosphatase capable of converting isopentenol into 3-methyl-3-butenol, (c) optionally the genetically modified host cell does not express, or has a decreased expression of one or more of NudB, phosphomevalonate kinase (PMK), and/or PMD, and (d) optionally one or more further enzymes capable of converting isopentenol and/or 3-methyl-3-butenol into a third compound, such as isoprene.

Inventors:

Lee, Taek Soon; Kang, Aram

Issue Date:

2023-05-30

Research Org.:

Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1998435

Patent Number(s):

11660961

Application Number:

17/067,610

Assignee:

The Regents of the University of California (Oakland, CA)

Patent Classifications (CPCs):

B - PERFORMING OPERATIONS B60 - VEHICLES IN GENERAL B60K - ARRANGEMENT OR MOUNTING OF PROPULSION UNITS OR OF TRANSMISSIONS IN VEHICLES

B - PERFORMING OPERATIONS B65 - CONVEYING B65G - TRANSPORT OR STORAGE DEVICES, e.g. CONVEYORS FOR LOADING OR TIPPING, SHOP CONVEYOR SYSTEMS OR PNEUMATIC TUBE CONVEYORS

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B - PERFORMING OPERATIONS B60 - VEHICLES IN GENERAL B60K - ARRANGEMENT OR MOUNTING OF PROPULSION UNITS OR OF TRANSMISSIONS IN VEHICLES

B60K2370/173 - Reversing assist
B60K35/00 - Arrangement of adaptations of instruments

B - PERFORMING OPERATIONS B65 - CONVEYING B65G - TRANSPORT OR STORAGE DEVICES, e.g. CONVEYORS FOR LOADING OR TIPPING, SHOP CONVEYOR SYSTEMS OR PNEUMATIC TUBE CONVEYORS

B65G69/006 - {Centring or aligning a vehicle at a loading station using means not being part of the vehicle}

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07F - ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM

C07F9/091 - {with hydroxyalkyl compounds with further substituents on alkyl}
C07F9/098 - {Esters of polyphosphoric acids or anhydrides}

C - CHEMISTRY C08 - ORGANIC MACROMOLECULAR COMPOUNDS C08F - MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS

C08F136/08 - Isoprene

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N9/0006 - {acting on CH-OH groups as donors
C12N9/1025 - {Acyltransferases
C12N9/1205 - {Phosphotransferases with an alcohol group as acceptor
C12N9/16 - acting on ester bonds
C12N9/88 - Lyases

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P5/007 - {containing one or more isoprene units, i.e. terpenes
C12P7/04 - acyclic

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES

C12Y101/01088 - Hydroxymethylglutaryl-CoA reductase
C12Y203/0301 - Hydroxymethylglutaryl-CoA synthase
C12Y207/01036 - Mevalonate kinase
C12Y401/01033 - Diphosphomevalonate decarboxylase

G - PHYSICS G01 - MEASURING G01S - RADIO DIRECTION-FINDING

G01S2013/9314 - {Parking operations}
G01S2013/9317 - {Driving backwards}
G01S2013/9323 - {Alternative operation using light waves}
G01S2013/9324 - {Alternative operation using ultrasonic waves}

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DOE Contract Number:  

AC02-05CH11231

Resource Type:

Patent

Resource Relation:

Patent File Date: 10/09/2020

Country of Publication:

United States

Language:

English