Polymerase-tethered nucleotides for nucleic acid synthesis
Abstract
Provided herein, among other things, is a conjugate comprising a polymerase and a nucleoside triphosphate, where the polymerase and the nucleoside triphosphate are covalently linked via a linker that comprises a cleavable linkage. A set of such conjugates, where the conjugates correspond to G, A, T (or U) and C is also provided. Methods for synthesizing a nucleic acid of a defined sequence are also provided. The conjugates can also be used for sequencing applications.
- Inventors:
- Issue Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1998282
- Patent Number(s):
- 11624079
- Application Number:
- 17/691,029
- Assignee:
- The Regents of the University of California (Oakland, CA)
- DOE Contract Number:
- AC02-05CH11231
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 03/09/2022
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Arlow, Daniel, and Palluk, Sebastian. Polymerase-tethered nucleotides for nucleic acid synthesis. United States: N. p., 2023.
Web.
Arlow, Daniel, & Palluk, Sebastian. Polymerase-tethered nucleotides for nucleic acid synthesis. United States.
Arlow, Daniel, and Palluk, Sebastian. Tue .
"Polymerase-tethered nucleotides for nucleic acid synthesis". United States. https://www.osti.gov/servlets/purl/1998282.
@article{osti_1998282,
title = {Polymerase-tethered nucleotides for nucleic acid synthesis},
author = {Arlow, Daniel and Palluk, Sebastian},
abstractNote = {Provided herein, among other things, is a conjugate comprising a polymerase and a nucleoside triphosphate, where the polymerase and the nucleoside triphosphate are covalently linked via a linker that comprises a cleavable linkage. A set of such conjugates, where the conjugates correspond to G, A, T (or U) and C is also provided. Methods for synthesizing a nucleic acid of a defined sequence are also provided. The conjugates can also be used for sequencing applications.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2023},
month = {4}
}
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Works referenced in this record:
Polymerase-tethered nucleoside triphosphates for use in nucleic acid synthesis
patent, February 2022
- Arlow, Daniel; Palluk, Sebastian
- US Patent Document 11,254,961