Rapid native single cell mass spectrometry
Abstract
A method for analyzing single cells by mass spectrometry includes the steps of providing a plurality of cells in a liquid medium and placing the cells and liquid medium in a single cell isolation and ejection system. Liquid medium containing a single cell is released from the single cell isolation and ejection system. The liquid medium and single cell are captured in a capture probe containing a flowing capture probe solvent. The cell is lysed by a lysis inducer in the capture probe to disperse single cell components into the medium. The lysed single cell components are transported to a mass spectrometer, where the lysed single cell components entering the mass spectrometer are spatially and temporally separated from any dispersed components of another single cell from the sample entering the mass spectrometer. Mass spectrometry is conducted on the lysed single-cell components. A system for analyzing single cells by mass spectrometry is also disclosed.
- Inventors:
- Issue Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1987237
- Patent Number(s):
- 11609235
- Application Number:
- 16/685,740
- Assignee:
- UT-Battelle LLC (Oak Ridge, TN)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12M - APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- AC05-00OR22725
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 11/15/2019
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Cahill, John F. Rapid native single cell mass spectrometry. United States: N. p., 2023.
Web.
Cahill, John F. Rapid native single cell mass spectrometry. United States.
Cahill, John F. Tue .
"Rapid native single cell mass spectrometry". United States. https://www.osti.gov/servlets/purl/1987237.
@article{osti_1987237,
title = {Rapid native single cell mass spectrometry},
author = {Cahill, John F.},
abstractNote = {A method for analyzing single cells by mass spectrometry includes the steps of providing a plurality of cells in a liquid medium and placing the cells and liquid medium in a single cell isolation and ejection system. Liquid medium containing a single cell is released from the single cell isolation and ejection system. The liquid medium and single cell are captured in a capture probe containing a flowing capture probe solvent. The cell is lysed by a lysis inducer in the capture probe to disperse single cell components into the medium. The lysed single cell components are transported to a mass spectrometer, where the lysed single cell components entering the mass spectrometer are spatially and temporally separated from any dispersed components of another single cell from the sample entering the mass spectrometer. Mass spectrometry is conducted on the lysed single-cell components. A system for analyzing single cells by mass spectrometry is also disclosed.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2023},
month = {3}
}
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Works referenced in this record:
Cell injector for flow cytometer having mass spectrometer detector and method for using same
patent-application, February 2011
- Tanner, Scott D.; Bandura, Dmitry R.; Baranov, Vladimir I.
- US Patent Application 12/521272; 20110024615
Capturing of cell fluid and analysis of its components under observation of cells and instruments for the cell fluid capturing and the analysis
patent-application, October 2014
- Masujima, Tsutomu; Tsuyama, Naohiro; Mizuno, Hajime
- US Patent Application 14/194555; 20140315237
Single-Cell Printer: Automated, On Demand, and Label Free
journal, December 2013
- Gross, Andre; Schöndube, Jonas; Niekrawitz, Sonja
- Journal of Laboratory Automation, Vol. 18, Issue 6
Laser dissection sampling modes for direct mass spectral analysis: Laser dissection sampling modes for mass spectral analysis
journal, February 2016
- Cahill, John F.; Kertesz, Vilmos; Van Berkel, Gary J.
- Rapid Communications in Mass Spectrometry, Vol. 30, Issue 5
Microfluidic devices for liquid chromatography-mass spectrometry and microscopic imaging
patent-application, October 2015
- Wang, Daojing; Mao, Pan; Gomez-Sjoberg, Rafael
- US Patent Application 14/729999; 20150293063
Online, Absolute Quantitation of Propranolol from Spatially Distinct 20- and 40-μm Dissections of Brain, Liver, and Kidney Thin Tissue Sections by Laser Microdissection–Liquid Vortex Capture–Mass Spectrometry
journal, May 2016
- Cahill, John F.; Kertesz, Vilmos; Weiskittel, Taylor M.
- Analytical Chemistry, Vol. 88, Issue 11
Capture probe
patent, August 2018
- Kertesz, Vilmos; Van Berkel, Gary J.
- US Patent Document 10,060,838
Capture Probe
patent-application, November 2019
- Kertesz, Vilmos; Van Berkel, Gary J.
- US Patent Application 16/517340; 20190339173
Elemental Flow Cytometer
patent-application, March 2014
- Bandura, Dmitry R.; Baranov, Vladimir I.; Tanner, Scott D.
- US Patent Application 14/016198; 20140070092
Label-free isolation and deposition of single bacterial cells from heterogeneous samples for clonal culturing
journal, September 2016
- Riba, J.; Gleichmann, T.; Zimmermann, S.
- Scientific Reports, Vol. 6, Issue 1
Molecular Genetic Characterization of Individual Cancer Cells Isolated via Single-Cell Printing
journal, September 2016
- Riba, Julian; Renz, Nathalie; Niemöller, Christoph
- PLOS ONE, Vol. 11, Issue 9
Cellular probe device, system and analysis method
patent-application, December 2015
- Yang, Zhibo; Burgett, Anthony W.; Liu, Shaorong
- US Patent Application 14/742485; 20150364306
Automated Optically Guided System for Chemical Analysis of Single Plant and Algae Cells Using Laser Microdissection/Liquid Vortex Capture/Mass Spectrometry
journal, August 2018
- Cahill, John F.; Kertesz, Vilmos
- Frontiers in Plant Science, Vol. 9