Methods and systems of characterizing and counting microbiological colonies
Abstract
Described herein are methods, systems, and non-transitory computer-readable media to non-destructively acquire three-dimensional profiles of cellular microbiological samples growing on the surface of a solid growth medium. Acquisitions can be performed by an optical microscope that includes a vertical scanning interferometer. The three-dimensional profiles can enable measurement of sample parameters of microcolonies, which can be made of microbial colony forming units. The methods and systems enable early and rapid detection and quantification of microbes.
- Inventors:
- Issue Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1986919
- Patent Number(s):
- 11525153
- Application Number:
- 16/039,681
- Assignee:
- Battelle Memorial Institute (Richland, WA)
- DOE Contract Number:
- AC05-76RL01830
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 07/19/2018
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Larimer, Curtis J., and Addleman, Raymond S. Methods and systems of characterizing and counting microbiological colonies. United States: N. p., 2022.
Web.
Larimer, Curtis J., & Addleman, Raymond S. Methods and systems of characterizing and counting microbiological colonies. United States.
Larimer, Curtis J., and Addleman, Raymond S. Tue .
"Methods and systems of characterizing and counting microbiological colonies". United States. https://www.osti.gov/servlets/purl/1986919.
@article{osti_1986919,
title = {Methods and systems of characterizing and counting microbiological colonies},
author = {Larimer, Curtis J. and Addleman, Raymond S.},
abstractNote = {Described herein are methods, systems, and non-transitory computer-readable media to non-destructively acquire three-dimensional profiles of cellular microbiological samples growing on the surface of a solid growth medium. Acquisitions can be performed by an optical microscope that includes a vertical scanning interferometer. The three-dimensional profiles can enable measurement of sample parameters of microcolonies, which can be made of microbial colony forming units. The methods and systems enable early and rapid detection and quantification of microbes.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2022},
month = {12}
}
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Works referenced in this record:
Rapid, Massively Parallel Single-Cell Drug Response Measurements via Live Cell Interferometry
patent-application, June 2014
- Reed, Jason C.; Teitell, Michael A.
- US Patent Application 14/235547; 20140178865
A Synthetic Biocidal Surface Comprising an Array of Nanospikes
patent-application, July 2016
- Juodkazis, Saulius; Ivanova, Elena
- US Patent Application 14/916,826; 2016/0212989 Al
Three-dimensional characterization of bacterial microcolonies on solid agar-based culture media
journal, February 2015
- Drazek, Laurent; Tournoud, Maud; Derepas, Frédéric
- Journal of Microbiological Methods, Vol. 109
Method for Measuring Single-Cell Biomass to Predict Clinical Outcomes for Stem Cell Transplant Patients
patent-application, September 2021
- Reed, Jason; Toor, Amir; Leslie, Kevin
- US Patent Application 16/330554; 20210278393
Method and Apparatus for Measuring Biofilm Thickness and Topology
patent-application, September 2016
- Larimer, Curtis J.; Suter, Jonathan D.; Addleman, Raymond S.
- US Patent Application 15/071921; 20160272933
Quantification of Biomass and Cell Motion in Human Pluripotent Stem Cell Colonies
journal, August 2013
- Zangle, Thomas A.; Chun, Jennifer; Zhang, Jin
- Biophysical Journal, Vol. 105, Issue 3
Rapid nondestructive measurement of bacterial cultures with 3D interferometric imaging
journal, May 2019
- Larimer, Curtis; Brann, Michelle R.; Powell, Joshua D.
- Scientific Reports, Vol. 9, Issue 1
Coating Compositions, Methods and Coated Devices
patent-application, November 2012
- Shukla, Anita; Hammond, Paula T.
- US Patent Application 13/459069; 20120277852
Optical Cytometry
patent-application, November 2010
- Teitell, Michael A.; Gimzewski, James K.; Reed, Jason C.
- US Patent Application 12/436702; 20100284016
Live-cell mass profiling: an emerging approach in quantitative biophysics
journal, November 2014
- Zangle, Thomas A.; Teitell, Michael A.
- Nature Methods, Vol. 11, Issue 12
In search of the microbe/mineral interface: quantitative analysis of bacteria on metal surfaces using vertical scanning interferometry
journal, June 2008
- Waters, M. S.; Sturm, C. A.; El-Naggar, M. Y.
- Geobiology, Vol. 6, Issue 3
Rapid Detection of Replicating Cells
patent-application, May 2003
- Straus, Don
- US Patent Application 10/236107; 20030082516
Identifying Desirable T Lymphocytes by Change in Mass Responses
patent-application, April 2016
- Teitell, Michael A.; Zangle, Thomas A.; White, Owen N.
- US Patent Application 14/890578; 20160103118