Engineering RuBisCo for food safety
Abstract
A method of improving a ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCo) to have a higher protein score is disclosed. The method includes the steps of: making a modified RbcL of the RuBisCo, by, on an RbcL unit of the RuBisCo, either substituting Met for Leu, Phe, Val, or Ile or combinations thereof; substituting Lys for Arg, Thr, or His or combinations thereof; or both of these substitutions. The modified RbcL consequently modifies the RuBisCo and is added to a biomass host where it is stable for homologous recombination. Plastid and nucleus integration was observed. Example RbcL sequences are disclosed with the desirable substitutions. The improved RuBisCo can be used as an improved proteinaceous food source for humans and animals.
- Inventors:
- Issue Date:
- Research Org.:
- Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
- Sponsoring Org.:
- USDOE National Nuclear Security Administration (NNSA)
- OSTI Identifier:
- 1986903
- Patent Number(s):
- 11518989
- Application Number:
- 17/038,513
- Assignee:
- National Technology & Engineering Solutions of Sandia, LLC (Albuquerque, NM); ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY (Scottsdale, AZ)
- DOE Contract Number:
- NA0003525
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 09/30/2020
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Davis, Ryan Wesley, Schoeniger, Joseph S., Varman, Arul M., Faisal, Muhammad, and Sarnaik, Aditya Pandharinath. Engineering RuBisCo for food safety. United States: N. p., 2022.
Web.
Davis, Ryan Wesley, Schoeniger, Joseph S., Varman, Arul M., Faisal, Muhammad, & Sarnaik, Aditya Pandharinath. Engineering RuBisCo for food safety. United States.
Davis, Ryan Wesley, Schoeniger, Joseph S., Varman, Arul M., Faisal, Muhammad, and Sarnaik, Aditya Pandharinath. Tue .
"Engineering RuBisCo for food safety". United States. https://www.osti.gov/servlets/purl/1986903.
@article{osti_1986903,
title = {Engineering RuBisCo for food safety},
author = {Davis, Ryan Wesley and Schoeniger, Joseph S. and Varman, Arul M. and Faisal, Muhammad and Sarnaik, Aditya Pandharinath},
abstractNote = {A method of improving a ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCo) to have a higher protein score is disclosed. The method includes the steps of: making a modified RbcL of the RuBisCo, by, on an RbcL unit of the RuBisCo, either substituting Met for Leu, Phe, Val, or Ile or combinations thereof; substituting Lys for Arg, Thr, or His or combinations thereof; or both of these substitutions. The modified RbcL consequently modifies the RuBisCo and is added to a biomass host where it is stable for homologous recombination. Plastid and nucleus integration was observed. Example RbcL sequences are disclosed with the desirable substitutions. The improved RuBisCo can be used as an improved proteinaceous food source for humans and animals.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2022},
month = {12}
}
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