Capture, disruption, and extraction apparatus and method
Abstract
A cell capture, disruption, and extraction method includes a introducing a plurality of abrasives in a disruption chamber, which can include diamond powder, variably and multi dimensionally disbursed therein, and a pestle positioned in the disruption chamber. The method includes agitating the abrasives by moving the disruption chamber and/or pestle, agitation of the abrasives tearing cell structure in the solution to access its contents. A binding column or size exclusion column can be positioned downstream of the disruption chamber. Cell solution can first be introduced in the disruption chamber, the abrasives capturing the cells and allowing therethrough and purging the waste content, then breaking the cell content. The lysate can then bind to an extraction matrix downstream of the disruption chamber or it can be mixed in with the abrasives.
- Inventors:
- Issue Date:
- Research Org.:
- US Dept. of Energy (USDOE), Washington, DC (United States)
- Sponsoring Org.:
- USDOE; National Aeronautics and Space Administration (NASA)
- OSTI Identifier:
- 1986838
- Patent Number(s):
- 11504709
- Application Number:
- 16/557,137
- Assignee:
- Pevyan, Kianoosh, Seattle, WA (United States)
- DOE Contract Number:
- NNA09DA96C
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 08/30/2019
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Peyvan, Kianoosh. Capture, disruption, and extraction apparatus and method. United States: N. p., 2022.
Web.
Peyvan, Kianoosh. Capture, disruption, and extraction apparatus and method. United States.
Peyvan, Kianoosh. Tue .
"Capture, disruption, and extraction apparatus and method". United States. https://www.osti.gov/servlets/purl/1986838.
@article{osti_1986838,
title = {Capture, disruption, and extraction apparatus and method},
author = {Peyvan, Kianoosh},
abstractNote = {A cell capture, disruption, and extraction method includes a introducing a plurality of abrasives in a disruption chamber, which can include diamond powder, variably and multi dimensionally disbursed therein, and a pestle positioned in the disruption chamber. The method includes agitating the abrasives by moving the disruption chamber and/or pestle, agitation of the abrasives tearing cell structure in the solution to access its contents. A binding column or size exclusion column can be positioned downstream of the disruption chamber. Cell solution can first be introduced in the disruption chamber, the abrasives capturing the cells and allowing therethrough and purging the waste content, then breaking the cell content. The lysate can then bind to an extraction matrix downstream of the disruption chamber or it can be mixed in with the abrasives.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2022},
month = {11}
}
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