Title: Multiplex RNA-guided genome engineering

Abstract

Methods of multiplex genome engineering in cells using Cas9 is provided which includes a cycle of steps of introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to the target DNA and which guide the enzyme to the target DNA, wherein the one or more RNAs and the enzyme are members of a co-localization complex for the target DNA, and introducing into the cell a second foreign nucleic acid encoding one or more donor nucleic acid sequences, and wherein the cycle is repeated a desired number of times to multiplex DNA engineering in cells.

Inventors:

Church, George M.; Dicarlo, James

Issue Date:

2022-10-04

Research Org.:

Harvard Univ., Cambridge, MA (United States)

Sponsoring Org.:

USDOE; National Science Foundation (NSF)

OSTI Identifier:

1986637

Patent Number(s):

11459585

Application Number:

14/903,719

Assignee:

President and Fellows of Harvard College (Cambridge, MA)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/111 - {General methods applicable to biologically active non-coding nucleic acids}
C12N15/902 - {using homologous recombination}
C12N2310/20 - involving clustered regularly interspaced short palindromic repeats [CRISPRs]

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DOE Contract Number:  

0540879; FG02-02ER63445

Resource Type:

Patent

Resource Relation:

Patent File Date: 07/08/2014

Country of Publication:

United States

Language:

English