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Title: Engineered hosts with exogenous ligninase and uses thereof

Abstract

The present invention relates to methods and engineered microbial hosts useful for treating lignin or a derivative thereof. In some embodiments, the host has one or more exogenous nucleic acid sequences that encode a ligninase (e.g., a laccase and/or a peroxidase).

Inventors:
;
Issue Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1925142
Patent Number(s):
11453895
Application Number:
16/838,340
Assignee:
National Technology & Engineering Solutions of Sandia, LLC (Albuquerque, NM)
Patent Classifications (CPCs):
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07G - COMPOUNDS OF UNKNOWN CONSTITUTION
C - CHEMISTRY C08 - ORGANIC MACROMOLECULAR COMPOUNDS C08H - DERIVATIVES OF NATURAL MACROMOLECULAR COMPOUNDS
DOE Contract Number:  
NA0003525
Resource Type:
Patent
Resource Relation:
Patent File Date: 04/02/2020
Country of Publication:
United States
Language:
English

Citation Formats

Singh, Seema, and Varman, Arul Mozhy. Engineered hosts with exogenous ligninase and uses thereof. United States: N. p., 2022. Web.
Singh, Seema, & Varman, Arul Mozhy. Engineered hosts with exogenous ligninase and uses thereof. United States.
Singh, Seema, and Varman, Arul Mozhy. Tue . "Engineered hosts with exogenous ligninase and uses thereof". United States. https://www.osti.gov/servlets/purl/1925142.
@article{osti_1925142,
title = {Engineered hosts with exogenous ligninase and uses thereof},
author = {Singh, Seema and Varman, Arul Mozhy},
abstractNote = {The present invention relates to methods and engineered microbial hosts useful for treating lignin or a derivative thereof. In some embodiments, the host has one or more exogenous nucleic acid sequences that encode a ligninase (e.g., a laccase and/or a peroxidase).},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2022},
month = {9}
}

Works referenced in this record:

Lignin peroxidase functionalities and prospective applications
journal, September 2016


Antioxidant activity applying an improved ABTS radical cation decolorization assay
journal, May 1999


Recombinant Laccase: I. Enzyme cloning and characterization
journal, January 2013


Stable and Functionally Diverse Versatile Peroxidases Designed Directly from Sequences
journal, February 2022


Engineering the ligninolytic enzyme consortium
journal, March 2015


Comparison of Fungal Laccases and Redox Mediators in Oxidation of a Nonphenolic Lignin Model Compound
journal, June 1999


Factors Influencing the Antioxidant Activity Determined by the ABTS•+Radical Cation Assay
journal, January 1997


Impact and prospective of fungal pre-treatment of lignocellulosic biomass for enzymatic hydrolysis
journal, January 2012


Structural Determinants of Oxidative Stabilization in an Evolved Versatile Peroxidase
journal, October 2014


Can laccases catalyze bond cleavage in lignin?
journal, January 2015


Laccase engineering: From rational design to directed evolution
journal, January 2015


Laccases and Their Applications: A Patent Review
journal, January 2008


Lignin-degrading enzymes
journal, February 2015


Effect of pH on the stability of Pleurotus eryngii versatile peroxidase during heterologous production in Emericella nidulans
journal, August 2004


Microbial Laccases and their Applications: A Review
journal, February 2011


Preparation of hydrophobic coatings
patent, February 2009


Lignin degradation: microorganisms, enzymes involved, genomes analysis and evolution
journal, October 2017


Laccase Variants Having Increased Expression and/or Activity
patent-application, May 2014


Synthesis of novel ionic liquids from lignin-derived compounds
patent, September 2017


Laboratory Evolution of High-Redox Potential Laccases
journal, September 2010


Structural Characterization of Lignin and Its Degradation Products with Spectroscopic Methods
journal, January 2017


Enzymatic lignocellulose hydrolysis: Improved cellulase productivity by insoluble solids recycling
journal, January 2013


Development of a strong intracellular expression system for Bacillus subtilis by optimizing promoter elements
journal, January 2012


Engineering Platforms for Directed Evolution of Laccase from Pycnoporus cinnabarinus
journal, December 2011


The making of versatile peroxidase by directed evolution
journal, August 2017


Development of chimeric laccases by directed evolution
journal, July 2012


Toward engineering E. coli with an autoregulatory system for lignin valorization
journal, March 2018


Assembly of evolved ligninolytic genes inSaccharomyces cerevisiae
journal, May 2014


Lignin biodegradation and industrial implications
journal, January 2014


High Redox Potential Peroxidases
book, January 2007


Directed evolution of a temperature-, peroxide- and alkaline pH-tolerant versatile peroxidase
journal, December 2011


Synthesis of novel ionic liquids from lignin-derived compounds
patent, December 2018


Laccase: a multi‐purpose biocatalyst at the forefront of biotechnology
journal, October 2016


Renewable aromatics from lignocellulosic lignin
patent, February 2019


Blood Tolerant Laccase by Directed Evolution
journal, February 2013


Decoding how a soil bacterium extracts building blocks and metabolic energy from ligninolysis provides road map for lignin valorization
journal, September 2016


Plant cell wall lignification and monolignol metabolism
journal, January 2013


Protein tolerance to random amino acid change
journal, June 2004


Renewable aromatics from lignocellulosic lignin
patent, March 2019


Saccharomyces cerevisiae in directed evolution: An efficient tool to improve enzymes
journal, May 2012


Laccases as a Potential Tool for the Efficient Conversion of Lignocellulosic Biomass: A Review
journal, May 2017


Alkaline versatile peroxidase by directed evolution
journal, January 2016


HMF production from glucose in ionic liquid media
patent, October 2018


OPTIMIZER: a web server for optimizing the codon usage of DNA sequences
journal, May 2007


Engineering and Applications of fungal laccases for organic synthesis
journal, January 2008