Engineered hosts with exogenous ligninase and uses thereof
Abstract
The present invention relates to methods and engineered microbial hosts useful for treating lignin or a derivative thereof. In some embodiments, the host has one or more exogenous nucleic acid sequences that encode a ligninase (e.g., a laccase and/or a peroxidase).
- Inventors:
- Issue Date:
- Research Org.:
- Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1925142
- Patent Number(s):
- 11453895
- Application Number:
- 16/838,340
- Assignee:
- National Technology & Engineering Solutions of Sandia, LLC (Albuquerque, NM)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07G - COMPOUNDS OF UNKNOWN CONSTITUTION
C - CHEMISTRY C08 - ORGANIC MACROMOLECULAR COMPOUNDS C08H - DERIVATIVES OF NATURAL MACROMOLECULAR COMPOUNDS
- DOE Contract Number:
- NA0003525
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 04/02/2020
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Singh, Seema, and Varman, Arul Mozhy. Engineered hosts with exogenous ligninase and uses thereof. United States: N. p., 2022.
Web.
Singh, Seema, & Varman, Arul Mozhy. Engineered hosts with exogenous ligninase and uses thereof. United States.
Singh, Seema, and Varman, Arul Mozhy. Tue .
"Engineered hosts with exogenous ligninase and uses thereof". United States. https://www.osti.gov/servlets/purl/1925142.
@article{osti_1925142,
title = {Engineered hosts with exogenous ligninase and uses thereof},
author = {Singh, Seema and Varman, Arul Mozhy},
abstractNote = {The present invention relates to methods and engineered microbial hosts useful for treating lignin or a derivative thereof. In some embodiments, the host has one or more exogenous nucleic acid sequences that encode a ligninase (e.g., a laccase and/or a peroxidase).},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2022},
month = {9}
}
Works referenced in this record:
Lignin Valorization: Two Hybrid Biochemical Routes for the Conversion of Polymeric Lignin into Value-added Chemicals
journal, August 2017
- Wu, Weihua; Dutta, Tanmoy; Varman, Arul M.
- Scientific Reports, Vol. 7, Issue 1
Lignin peroxidase functionalities and prospective applications
journal, September 2016
- Falade, Ayodeji O.; Nwodo, Uchechukwu U.; Iweriebor, Benson C.
- MicrobiologyOpen, Vol. 6, Issue 1
Antioxidant activity applying an improved ABTS radical cation decolorization assay
journal, May 1999
- Re, Roberta; Pellegrini, Nicoletta; Proteggente, Anna
- Free Radical Biology and Medicine, Vol. 26, Issue 9-10
Development of a Saccharomyces cerevisiae Strain with Enhanced Resistance to Phenolic Fermentation Inhibitors in Lignocellulose Hydrolysates by Heterologous Expression of Laccase
journal, March 2001
- Larsson, S.; Cassland, P.; Jonsson, L. J.
- Applied and Environmental Microbiology, Vol. 67, Issue 3, p. 1163-1170
Recombinant Laccase: I. Enzyme cloning and characterization
journal, January 2013
- Nicolini, Claudio; Bruzzese, Debora; Cambria, Maria Teresa
- Journal of Cellular Biochemistry, Vol. 114, Issue 3
Stable and Functionally Diverse Versatile Peroxidases Designed Directly from Sequences
journal, February 2022
- Barber-Zucker, Shiran; Mindel, Vladimir; Garcia-Ruiz, Eva
- Journal of the American Chemical Society, Vol. 144, Issue 8
Engineering the ligninolytic enzyme consortium
journal, March 2015
- Alcalde, Miguel
- Trends in Biotechnology, Vol. 33, Issue 3
Comparison of Fungal Laccases and Redox Mediators in Oxidation of a Nonphenolic Lignin Model Compound
journal, June 1999
- Li, Kaichang; Xu, Feng; Eriksson, Karl-Erik L.
- Applied and Environmental Microbiology, Vol. 65, Issue 6
Factors Influencing the Antioxidant Activity Determined by the ABTS•+Radical Cation Assay
journal, January 1997
- Miller, Nicholas J.; Rice-Evans, Catherine A.
- Free Radical Research, Vol. 26, Issue 3
Impact and prospective of fungal pre-treatment of lignocellulosic biomass for enzymatic hydrolysis
journal, January 2012
- Tian, Xiao-fei; Fang, Zhen; Guo, Feng
- Biofuels, Bioproducts and Biorefining, Vol. 6, Issue 3
Structural Determinants of Oxidative Stabilization in an Evolved Versatile Peroxidase
journal, October 2014
- Gonzalez-Perez, David; Garcia-Ruiz, Eva; Ruiz-Dueñas, Francisco Javier
- ACS Catalysis, Vol. 4, Issue 11
Can laccases catalyze bond cleavage in lignin?
journal, January 2015
- Munk, Line; Sitarz, Anna K.; Kalyani, Dayanand C.
- Biotechnology Advances, Vol. 33, Issue 1
Laccase engineering: From rational design to directed evolution
journal, January 2015
- Mate, Diana M.; Alcalde, Miguel
- Biotechnology Advances, Vol. 33, Issue 1
Systematic Screening of All Signal Peptides from Bacillus subtilis: A Powerful Strategy in Optimizing Heterologous Protein Secretion in Gram-positive Bacteria
journal, September 2006
- Brockmeier, Ulf; Caspers, Michael; Freudl, Roland
- Journal of Molecular Biology, Vol. 362, Issue 3
Laccases and Their Applications: A Patent Review
journal, January 2008
- Kunamneni, Adinarayana; Plou, Francisco; Ballesteros, Antonio
- Recent Patents on Biotechnology, Vol. 2, Issue 1
Apparatus and method for measuring single cell and sub-cellular photosynthetic efficiency
patent, July 2013
- Davis, Ryan Wesley; Singh, Seema; Wu, Huawen
- US Patent Document 8,481,974
Lignin-degrading enzymes
journal, February 2015
- Pollegioni, Loredano; Tonin, Fabio; Rosini, Elena
- FEBS Journal, Vol. 282, Issue 7
Characterization of a gene encoding Trametes versicolor laccase A and improved heterologous expression in Saccharomyces cerevisiae by decreased cultivation temperature
journal, September 1999
- Cassland, P.; Jönsson, L. J.
- Applied Microbiology and Biotechnology, Vol. 52, Issue 3
Effect of pH on the stability of Pleurotus eryngii versatile peroxidase during heterologous production in Emericella nidulans
journal, August 2004
- L�-Chau, T. A.; Ruiz-Due�as, F. J.; Camarero, S.
- Bioprocess and Biosystems Engineering, Vol. 26, Issue 5
Microbial Laccases and their Applications: A Review
journal, February 2011
- Desai, S. S.; Nityanand, C.
- Asian Journal of Biotechnology, Vol. 3, Issue 2
Preparation of hydrophobic coatings
patent, February 2009
- Branson, Eric D.; Shah, Pratik; Singh, Seema
- US Patent Document 7,485,343
Lignin degradation: microorganisms, enzymes involved, genomes analysis and evolution
journal, October 2017
- Janusz, Grzegorz; Pawlik, Anna; Sulej, Justyna
- FEMS Microbiology Reviews, Vol. 41, Issue 6
Complete Sequence of the First Chimera Genome Constructed by Cloning the Whole Genome of Synechocystis Strain PCC6803 into the Bacillus subtilis 168 Genome
journal, December 2012
- Watanabe, Satoru; Shiwa, Yuh; Itaya, Mitsuhiro
- Journal of Bacteriology, Vol. 194, Issue 24
Laccase Variants Having Increased Expression and/or Activity
patent-application, May 2014
- Wang, Huaming; Ward, Michael
- US Patent Application 14/110123; 20140123404
Synthesis of novel ionic liquids from lignin-derived compounds
patent, September 2017
- Socha, Aaron; Singh, Seema; Simmons, Blake A.
- US Patent Document 9,765,044
Laboratory Evolution of High-Redox Potential Laccases
journal, September 2010
- Maté, Diana; García-Burgos, Carlos; García-Ruiz, Eva
- Chemistry & Biology, Vol. 17, Issue 9
Structural Characterization of Lignin and Its Degradation Products with Spectroscopic Methods
journal, January 2017
- Lu, Yao; Lu, Yong-Chao; Hu, Hong-Qin
- Journal of Spectroscopy, Vol. 2017
Development of Pgrac100-based expression vectors allowing high protein production levels in Bacillus subtilis and relatively low basal expression in Escherichia coli
journal, May 2015
- Phan, Trang Thi Phuong; Tran, Linh Thuoc; Schumann, Wolfgang
- Microbial Cell Factories, Vol. 14, Issue 1
Enzymatic lignocellulose hydrolysis: Improved cellulase productivity by insoluble solids recycling
journal, January 2013
- Weiss, Noah; Börjesson, Johan; Pedersen, Lars Saaby
- Biotechnology for Biofuels, Vol. 6, Issue 1
Development of a strong intracellular expression system for Bacillus subtilis by optimizing promoter elements
journal, January 2012
- Phan, Trang Thi Phuong; Nguyen, Hoang Duc; Schumann, Wolfgang
- Journal of Biotechnology, Vol. 157, Issue 1
Engineering Platforms for Directed Evolution of Laccase from Pycnoporus cinnabarinus
journal, December 2011
- Camarero, S.; Pardo, I.; Cañas, A. I.
- Applied and Environmental Microbiology, Vol. 78, Issue 5
The making of versatile peroxidase by directed evolution
journal, August 2017
- Gonzalez-Perez, David; Alcalde, Miguel
- Biocatalysis and Biotransformation, Vol. 36, Issue 1
Development of chimeric laccases by directed evolution
journal, July 2012
- Pardo, Isabel; Vicente, Ana Isabel; Mate, Diana M.
- Biotechnology and Bioengineering, Vol. 109, Issue 12
Toward engineering E. coli with an autoregulatory system for lignin valorization
journal, March 2018
- Wu, Weihua; Liu, Fang; Singh, Seema
- Proceedings of the National Academy of Sciences, Vol. 115, Issue 12
Improvement of hydrogen peroxide stability of Pleurotus eryngii versatile ligninolytic peroxidase by rational protein engineering
journal, January 2014
- Bao, Xue; Huang, Xuenian; Lu, Xuefeng
- Enzyme and Microbial Technology, Vol. 54
Assembly of evolved ligninolytic genes inSaccharomyces cerevisiae
journal, May 2014
- Gonzalez-Perez, David; Alcalde, Miguel
- Bioengineered, Vol. 5, Issue 4
Lignin biodegradation and industrial implications
journal, January 2014
- B. Fisher, Adam; S. Fong, Stephen
- AIMS Bioengineering, Vol. 1, Issue 2
Laccases: Biological Functions, Molecular Structure and Industrial Applications
book, January 2007
- Alcalde, Miguel
- Industrial Enzymes
Directed evolution of a temperature-, peroxide- and alkaline pH-tolerant versatile peroxidase
journal, December 2011
- Garcia-Ruiz, Eva; Gonzalez-Perez, David; Ruiz-Dueñas, Francisco J.
- Biochemical Journal, Vol. 441, Issue 1
Synthesis of novel ionic liquids from lignin-derived compounds
patent, December 2018
- Socha, Aaron; Singh, Seema; Simmons, Blake A.
- US Patent Document 10,155,735
Laccase: a multi‐purpose biocatalyst at the forefront of biotechnology
journal, October 2016
- Mate, Diana M.; Alcalde, Miguel
- Microbial Biotechnology, Vol. 10, Issue 6
Renewable aromatics from lignocellulosic lignin
patent, February 2019
- Singh, Seema; Varanasi, Patanjali; Simmons, Blake A.
- US Patent Document 10,208,076
Blood Tolerant Laccase by Directed Evolution
journal, February 2013
- Mate, Diana M.; Gonzalez-Perez, David; Falk, Magnus
- Chemistry & Biology, Vol. 20, Issue 2
Decoding how a soil bacterium extracts building blocks and metabolic energy from ligninolysis provides road map for lignin valorization
journal, September 2016
- Varman, Arul M.; He, Lian; Follenfant, Rhiannon
- Proceedings of the National Academy of Sciences, Vol. 113, Issue 40
Multiplexed screening of enzyme activities using nanostructure-initiator mass spectrometry
patent, March 2019
- Northen, Trent R.; Reindl, Wolfgang E.; Deng, Kai
- US Patent Document 10,240,180
Plant cell wall lignification and monolignol metabolism
journal, January 2013
- Wang, Yin; Chantreau, Maxime; Sibout, Richard
- Frontiers in Plant Science, Vol. 4
Protein tolerance to random amino acid change
journal, June 2004
- Guo, H. H.; Choe, J.; Loeb, L. A.
- Proceedings of the National Academy of Sciences, Vol. 101, Issue 25, p. 9205-9210
Comparative antioxidant activities of carotenoids measured by ferric reducing antioxidant power (FRAP), ABTS bleaching assay (αTEAC), DPPH assay and peroxyl radical scavenging assay
journal, November 2011
- Müller, Lars; Fröhlich, Kati; Böhm, Volker
- Food Chemistry, Vol. 129, Issue 1
Renewable aromatics from lignocellulosic lignin
patent, March 2019
- Singh, Seema; Varanasi, Patanjali; Simmons, Blake A.
- US Patent Document 10,233,292
Saccharomyces cerevisiae in directed evolution: An efficient tool to improve enzymes
journal, May 2012
- Gonzalez-Perez, David; Garcia-Ruiz, Eva; Alcalde, Miguel
- Bioengineered, Vol. 3, Issue 3
Laccases as a Potential Tool for the Efficient Conversion of Lignocellulosic Biomass: A Review
journal, May 2017
- Fillat, Úrsula; Ibarra, David; Eugenio, María
- Fermentation, Vol. 3, Issue 2
Alkaline versatile peroxidase by directed evolution
journal, January 2016
- Gonzalez-Perez, David; Mateljak, Ivan; Garcia-Ruiz, Eva
- Catalysis Science & Technology, Vol. 6, Issue 17
HMF production from glucose in ionic liquid media
patent, October 2018
- Sathitsuksanoh, Noppadon; Travisano, Philip; George, Anthe
- US Patent Document 10,112,916
OPTIMIZER: a web server for optimizing the codon usage of DNA sequences
journal, May 2007
- Puigbo, P.; Guzman, E.; Romeu, A.
- Nucleic Acids Research, Vol. 35, Issue Web Server
Engineering and Applications of fungal laccases for organic synthesis
journal, January 2008
- Kunamneni, Adinarayana; Camarero, Susana; García-Burgos, Carlos
- Microbial Cell Factories, Vol. 7, Issue 1