CasZ compositions and methods of use

Doudna, Jennifer A.; Burstein, David; Chen, Janice S.; Harrington, Lucas B.; Paez-Espino, David; Banfield, Jillian F.

Title: CasZ compositions and methods of use

Abstract

Provided are compositions and methods that include one or more of: (1) a “CasZ” protein (also referred to as a CasZ polypeptide), a nucleic acid encoding the CasZ protein, and/or a modified host cell comprising the CasZ protein (and/or a nucleic acid encoding the same); (2) a CasZ guide RNA that binds to and provides sequence specificity to the CasZ protein, a nucleic acid encoding the CasZ guide RNA, and/or a modified host cell comprising the CasZ guide RNA (and/or a nucleic acid encoding the same); and (3) a CasZ transactivating noncoding RNA (trancRNA) (referred to herein as a “CasZ trancRNA”), a nucleic acid encoding the CasZ trancRNA, and/or a modified host cell comprising the CasZ trancRNA (and/or a nucleic acid encoding the same).

Inventors:: Doudna, Jennifer A.; Burstein, David; Chen, Janice S.; Harrington, Lucas B.; Paez-Espino, David; Banfield, Jillian F.

Issue Date:: 2022-09-13

Research Org.:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1925094

Patent Number(s):: 11441137

Application Number:: 16/884,766

Assignee:: The Regents of the University of California (Oakland, CA)

Patent Classifications (CPCs):: C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C07K2319/09 - containing a nuclear localisation signal

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/102 - {Mutagenizing nucleic acids}
C12N15/11 - DNA or RNA fragments
C12N15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
C12N15/63 - Introduction of foreign genetic material using vectors
C12N2310/20 - involving clustered regularly interspaced short palindromic repeats [CRISPRs]
C12N2800/80 - Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
C12N9/22 - Ribonucleases {RNAses, DNAses

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C12Q1/6809 - Methods for determination or identification of nucleic acids involving differential detection
C12Q1/6816 - characterised by the detection means
C12Q2521/301 - Endonuclease

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DOE Contract Number:: 1244557; AC02-05CH11231

Resource Type:: Patent

Resource Relation:: Patent File Date: 05/27/2020

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Doudna, Jennifer A., Burstein, David, Chen, Janice S., Harrington, Lucas B., Paez-Espino, David, and Banfield, Jillian F. CasZ compositions and methods of use.  United States: N. p., 2022. 
        Web.

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                    Doudna, Jennifer A., Burstein, David, Chen, Janice S., Harrington, Lucas B., Paez-Espino, David, & Banfield, Jillian F. CasZ compositions and methods of use.  United States.

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                    Doudna, Jennifer A., Burstein, David, Chen, Janice S., Harrington, Lucas B., Paez-Espino, David, and Banfield, Jillian F. Tue .  
        "CasZ compositions and methods of use".  United States.  https://www.osti.gov/servlets/purl/1925094.

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@article{osti_1925094,

  title        = {CasZ compositions and methods of use},

  author       = {Doudna, Jennifer A. and Burstein, David and Chen, Janice S. and Harrington, Lucas B. and Paez-Espino, David and Banfield, Jillian F.},

  abstractNote = {Provided are compositions and methods that include one or more of: (1) a “CasZ” protein (also referred to as a CasZ polypeptide), a nucleic acid encoding the CasZ protein, and/or a modified host cell comprising the CasZ protein (and/or a nucleic acid encoding the same); (2) a CasZ guide RNA that binds to and provides sequence specificity to the CasZ protein, a nucleic acid encoding the CasZ guide RNA, and/or a modified host cell comprising the CasZ guide RNA (and/or a nucleic acid encoding the same); and (3) a CasZ transactivating noncoding RNA (trancRNA) (referred to herein as a “CasZ trancRNA”), a nucleic acid encoding the CasZ trancRNA, and/or a modified host cell comprising the CasZ trancRNA (and/or a nucleic acid encoding the same).},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2022},

  month        = {9}

}

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