Method of regulating gene expression in a cell
Abstract
The disclosure provides methods of making a tetracycline inducible expression system in a cell. The methods include providing the cell with a first nucleic acid sequence comprising a first promoter operably linked to a tetracycline repressor gene coding sequence, providing the cell with a second nucleic acid sequence comprising a second promoter operably linked to a coding sequence of a gene of interest wherein the second promoter is modified to include one or more tetracycline repressor protein binding sites, and determining the expression of the gene of interest in the presence or absence of tetracycline. The disclosure further provides nucleic acid sequences, vectors and cells including the tetracycline inducible modified promoter.
- Inventors:
- Issue Date:
- Research Org.:
- Harvard Univ., Cambridge, MA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1924887
- Patent Number(s):
- 11384358
- Application Number:
- 16/498,465
- Assignee:
- President and Fellows of Harvard College (Cambridge, MA)
- Patent Classifications (CPCs):
-
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- FG02-02ER63445
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 03/29/2018
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Debnath, Anik, Juarez, Javier Fernandez, Lee, Henry Hung-yi, and Church, George M. Method of regulating gene expression in a cell. United States: N. p., 2022.
Web.
Debnath, Anik, Juarez, Javier Fernandez, Lee, Henry Hung-yi, & Church, George M. Method of regulating gene expression in a cell. United States.
Debnath, Anik, Juarez, Javier Fernandez, Lee, Henry Hung-yi, and Church, George M. Tue .
"Method of regulating gene expression in a cell". United States. https://www.osti.gov/servlets/purl/1924887.
@article{osti_1924887,
title = {Method of regulating gene expression in a cell},
author = {Debnath, Anik and Juarez, Javier Fernandez and Lee, Henry Hung-yi and Church, George M.},
abstractNote = {The disclosure provides methods of making a tetracycline inducible expression system in a cell. The methods include providing the cell with a first nucleic acid sequence comprising a first promoter operably linked to a tetracycline repressor gene coding sequence, providing the cell with a second nucleic acid sequence comprising a second promoter operably linked to a coding sequence of a gene of interest wherein the second promoter is modified to include one or more tetracycline repressor protein binding sites, and determining the expression of the gene of interest in the presence or absence of tetracycline. The disclosure further provides nucleic acid sequences, vectors and cells including the tetracycline inducible modified promoter.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2022},
month = {7}
}
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