Title: Method of regulating gene expression in a cell

Abstract

The disclosure provides methods of making a tetracycline inducible expression system in a cell. The methods include providing the cell with a first nucleic acid sequence comprising a first promoter operably linked to a tetracycline repressor gene coding sequence, providing the cell with a second nucleic acid sequence comprising a second promoter operably linked to a coding sequence of a gene of interest wherein the second promoter is modified to include one or more tetracycline repressor protein binding sites, and determining the expression of the gene of interest in the presence or absence of tetracycline. The disclosure further provides nucleic acid sequences, vectors and cells including the tetracycline inducible modified promoter.

Inventors:

Debnath, Anik; Juarez, Javier Fernandez; Lee, Henry Hung-yi; Church, George M.

Issue Date:

Tue Jul 12 00:00:00 EDT 2022

Research Org.:

Harvard Univ., Cambridge, MA (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1924887

Patent Number(s):

11384358

Application Number:

16/498,465

Assignee:

President and Fellows of Harvard College (Cambridge, MA)

Patent Classifications (CPCs):

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

Show more

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

A61P29/00 - Non-central analgesic, antipyretic or anti-inflammatory agents, e.g antirheumatic agents

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N1/20 - Bacteria {
C12N15/635 - {Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline}
C12N15/746 - {for lactic acid bacteria

Show less

DOE Contract Number:  

FG02-02ER63445

Resource Type:

Patent

Resource Relation:

Patent File Date: 03/29/2018

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES