Title: Probe for selectively characterizing enzymes involved in xenobiotic metabolism and method of making and using the same

Abstract

Activity-based probes that can be used to selectively identify and characterize enzymes that are involved in different phases of xenobiotic metabolism in a host and its microbiota population(s) are described. The activity-based probes described specifically label only their target active enzymes involved in xenobiotic metabolism and therefore provide a measurement of true protein functional activity rather than transcript or protein abundance. The activity-based probes also provide multimodal profiling of these active enzymes. Methods for preparing the activity based probes and exemplary methods for their use also are disclosed.

Inventors:

Wright, Aaron T.; Ramos-Hunter, Susan; Whidbey, Christopher

Issue Date:

2022-05-31

Research Org.:

Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1892986

Patent Number(s):

11345950

Application Number:

16/151,170

Assignee:

Battelle Memorial Institute (Richland, WA)

Patent Classifications (CPCs):

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07C - ACYCLIC OR CARBOCYCLIC COMPOUNDS

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07D - HETEROCYCLIC COMPOUNDS

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C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07C - ACYCLIC OR CARBOCYCLIC COMPOUNDS

C07C203/04 - having nitrate groups bound to acyclic carbon atoms
C07C233/20 - having the carbon atom of the carboxamide group bound to a carbon atom of an acyclic unsaturated carbon skeleton
C07C233/33 - with the substituted hydrocarbon radical bound to the nitrogen atom of the carboxamide group by a carbon atom of a six-membered aromatic ring
C07C247/00 - Compounds containing azido groups
C07C247/12 - being further substituted by carboxyl groups
C07C271/28 - to a carbon atom of a non-condensed six-membered aromatic ring
C07C305/22 - having oxygen atoms of sulfate groups bound to carbon atoms of six-membered aromatic rings
C07C317/40 - Y being a hydrogen or a carbon atom

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07D - HETEROCYCLIC COMPOUNDS

C07D203/26 - condensed with carbocyclic rings or ring systems
C07D311/16 - substituted in position 7
C07D405/04 - directly linked by a ring-member-to-ring-member bond
C07D405/12 - linked by a chain containing hetero atoms as chain links

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS

C07H15/203 - Monocyclic carbocyclic rings other than cyclohexane rings
C07H19/067 - with ribosyl as the saccharide radical

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K1/13 - Labelling of peptides

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q1/34 - involving hydrolase
C12Q1/48 - involving transferase
C12Q1/6837 - using probe arrays or probe chips
C12Q1/6841 - In situ hybridisation
C12Q2565/514 - characterised by the use of the arrayed oligonucleotides as identifier tags, e.g. universal addressable array, anti-tag or tag complement array
C12Q2600/106 - Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N2333/91177 - Glutathione transferases
G01N33/582 - {with fluorescent label}
G01N33/583 - {with non-fluorescent dye label}

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DOE Contract Number:  

AC05-76RL01830

Resource Type:

Patent

Resource Relation:

Patent File Date: 10/03/2018

Country of Publication:

United States

Language:

English