DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Systems and methods for interferometric multifocus microscopy

Abstract

A system to generate image representations includes a first objective that receives a first light beam emitted from a sample and a second objective that receives a second light beam emitted from the sample, where the first light beam and the second light beam have conjugate phase. The system also includes a first diffractive element to receive the first light beam and separate it into a first plurality of diffractive light beams that are spatially distinct, and a second diffractive element to receive the second light beam and separate it into a second plurality of diffractive light beams that are spatially distinct. The system further includes a detector that receives the first and second plurality of diffractive light beams. The first plurality of diffractive light beams and the second plurality of diffractive light beams are simultaneously directed and focused onto different portions of an image plane of the detector.

Inventors:
; ; ; ;
Issue Date:
Research Org.:
Argonne National Laboratory (ANL), Argonne, IL (United States); Northwestern Univ., Evanston, IL (United States); Univ. of Chicago, IL (United States)
Sponsoring Org.:
USDOE; National Science Foundation (NSF); US Office of Naval Research (ONR)
OSTI Identifier:
1892960
Patent Number(s):
11340057
Application Number:
15/734,172
Assignee:
Northwestern University (Evanston, IL); UChicago Argonne, LLC (Chicago, IL); The University of Chicago (Chicago, IL)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01B - MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
AC02-06CH11357; IIS-1453192; N00014-15-1-2735
Resource Type:
Patent
Resource Relation:
Patent File Date: 06/21/2019
Country of Publication:
United States
Language:
English

Citation Formats

He, Kuan, Cossairt, Oliver, Katsaggelos, Aggelos K., Scherer, Norbert, and Hereld, Mark. Systems and methods for interferometric multifocus microscopy. United States: N. p., 2022. Web.
He, Kuan, Cossairt, Oliver, Katsaggelos, Aggelos K., Scherer, Norbert, & Hereld, Mark. Systems and methods for interferometric multifocus microscopy. United States.
He, Kuan, Cossairt, Oliver, Katsaggelos, Aggelos K., Scherer, Norbert, and Hereld, Mark. Tue . "Systems and methods for interferometric multifocus microscopy". United States. https://www.osti.gov/servlets/purl/1892960.
@article{osti_1892960,
title = {Systems and methods for interferometric multifocus microscopy},
author = {He, Kuan and Cossairt, Oliver and Katsaggelos, Aggelos K. and Scherer, Norbert and Hereld, Mark},
abstractNote = {A system to generate image representations includes a first objective that receives a first light beam emitted from a sample and a second objective that receives a second light beam emitted from the sample, where the first light beam and the second light beam have conjugate phase. The system also includes a first diffractive element to receive the first light beam and separate it into a first plurality of diffractive light beams that are spatially distinct, and a second diffractive element to receive the second light beam and separate it into a second plurality of diffractive light beams that are spatially distinct. The system further includes a detector that receives the first and second plurality of diffractive light beams. The first plurality of diffractive light beams and the second plurality of diffractive light beams are simultaneously directed and focused onto different portions of an image plane of the detector.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2022},
month = {5}
}

Works referenced in this record:

Elucidation of intracellular recycling pathways leading to exocytosis of the Fc receptor, FcRn, by using multifocal plane microscopy
journal, March 2007


Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure
journal, February 2009


Fighting against diffraction: apodization and near field diffraction structures
journal, September 2011


Methods and Systems for Determining a Presence of Macro and Micro Defects on a Specimen
patent-application, May 2004


Systems and methods for 3-dimensional interferometric microscopy
patent-application, July 2008


3D Image Reconstruction from Multi-Focus Microscope: Axial Super-Resolution and Multiple-Frame Processing
conference, April 2018


Multiplane imaging and three dimensional nanoscale particle tracking in biological microscopy
journal, January 2010


Sevenfold improvement of axial resolution in 3D wide-field microscopy using two objective lenses
conference, March 1995

  • Gustafsson, Mats G. L.; Agard, David A.; Sedat, John W.
  • IS&T/SPIE's Symposium on Electronic Imaging: Science & Technology, SPIE Proceedings
  • https://doi.org/10.1117/12.205334

Comparison of I5M and 4Pi-microscopy
journal, May 2006


Three-dimensional microscope and method for obtaining three-dimensional image
patent-application, October 2008


Designing the focal plane spacing for multifocal plane microscopy
journal, January 2014


Multifocus structured illumination microscopy for fast volumetric super-resolution imaging
journal, August 2017


DeconvolutionLab2: An open-source software for deconvolution microscopy
journal, February 2017


Simultaneous Imaging of Different Focal Planes in Fluorescence Microscopy for the Study of Cellular Dynamics in Three Dimensions
journal, December 2004


Evaluation of signals of fluorescence scanning microscopy using a confocal laser scanning microscope
patent-application, April 2018


Coherent use of opposing lenses for axial resolution increase in fluorescence microscopy I Comparative study of concepts
journal, January 2001


Improved single particle localization accuracy with dual objective multifocal plane microscopy
journal, January 2009


Apparatus for detecting defects on a mask
patent, November 1998


Visualization and Tracking of Single Protein Molecules in the Cell Nucleus
journal, June 2001


Diffraction-unlimited three-dimensional optical nanoscopy with opposing lenses
journal, July 2009


MultiFocus Polarization Microscope (MF-PolScope) for 3D polarization imaging of up to 25 focal planes simultaneously
journal, January 2015


Systems, Methods, and Apparatuses of Low-coherence Enhanced Backscattering Spectroscopy
patent-application, February 2008


Enhancement of axial resolution in fluorescence microscopy by standing-wave excitation
journal, November 1993


Isotropic 3D Nanoscopy based on single emitter switching
journal, January 2008


I5S: Wide-Field Light Microscopy with 100-nm-Scale Resolution in Three Dimensions
journal, June 2008


Understanding diffractive optic design in the scalar domain
journal, January 1995


Richardson–Lucy algorithm with total variation regularization for 3D confocal microscope deconvolution
journal, January 2006


Simultaneous multiplane imaging with a distorted diffraction grating
journal, January 1999


Imaging and Tracking of Single GFP Molecules in Solution
journal, April 2000


Fast multicolor 3D imaging using aberration-corrected multifocus microscopy
journal, December 2012


Two-color nanoscopy of three-dimensional volumes by 4Pi detection of stochastically switched fluorophores
journal, March 2011