Conversion of biomass to useful intermediates

Beckham, Gregg Tyler; Johnson, Christopher W.; Vardon, Derek Richard

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A - human necessities
A01 - agriculture
A21 - baking
A22 - butchering
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Title: Conversion of biomass to useful intermediates

Abstract

An aspect of the present disclosure is a microbial cell that includes a genetic modification resulting in the expression of a deficient form of an endogenous dioxygenase, and a gene encoding an exogenous dioxygenase and a promoter sequence, where the endogenous dioxygenase includes PcaH and PcaG, the exogenous dioxygenase includes LigA and LigB, the microbial cell is capable of growth utilizing at least one of a cellulose decomposition molecule or a lignin decomposition molecule, and the microbial cell is capable of producing 2-hydroxy-2H-pyran-4,6-dicarboxylic acid.

Inventors:: Beckham, Gregg Tyler; Johnson, Christopher W.; Vardon, Derek Richard

Issue Date:: 2022-05-10

Research Org.:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1892914

Patent Number(s):: 11326190

Application Number:: 16/263,867

Assignee:: Alliance for Sustainable Energy, LLC (Golden, CO)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/52 - Genes encoding for enzymes or proenzymes
C12N9/0006 - {acting on CH-OH groups as donors
C12N9/0008 - {acting on the aldehyde or oxo group of donors
C12N9/0069 - {acting on single donors with incorporation of molecular oxygen, i.e. oxygenases
C12N9/13 - {transferring sulfur containing groups
C12N9/18 - Carboxylic ester hydrolases {
C12N9/88 - Lyases
C12N9/90 - Isomerases

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P17/06 - containing a six-membered hetero ring, e.g. fluorescein
C12P7/44 - Polycarboxylic acids

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C12Y101/01312 - 2-Hydroxy-4-carboxymuconate semialdehyde hemiacetal dehydrogenase
C12Y102/01085 - 2-Hydroxymuconate-6-semialdehyde dehydrogenase
C12Y113/11001 - Catechol 1,2-dioxygenase
C12Y113/11003 - Protocatechuate 3,4-dioxygenase
C12Y113/11008 - Protocatechuate 4,5-dioxygenase
C12Y208/03006 - 3-Oxoadipate CoA-transferase
C12Y301/01024 - 3-Oxoadipate enol-lactonase
C12Y301/01057 - 2-Pyrone-4,6-dicarboxylate lactonase
C12Y307/01009 - 2-Hydroxymuconate-semialdehyde hydrolase
C12Y401/01044 - 4-Carboxymuconolactone decarboxylase
C12Y401/01045 - Aminocarboxymuconate-semialdehyde decarboxylase
C12Y401/01077 - 2-Oxo-3-hexenedioate decarboxylase
C12Y402/01083 - 4-Oxalmesaconate hydratase
C12Y503/02 - interconverting keto- and enol-groups
C12Y503/02006 - 2-Hydroxymuconate tautomerase
C12Y503/03004 - Muconolactone DELTA-isomerase
C12Y505/01001 - Muconate cycloisomerase
C12Y505/01002 - 3-Carboxy-cis,cis-muconate cycloisomerase

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DOE Contract Number:: AC36-08GO28308

Resource Type:: Patent

Resource Relation:: Patent File Date: 01/31/2019

Country of Publication:: United States

Language:: English

Citation Formats


                    Beckham, Gregg Tyler, Johnson, Christopher W., and Vardon, Derek Richard. Conversion of biomass to useful intermediates.  United States: N. p., 2022. 
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                    Beckham, Gregg Tyler, Johnson, Christopher W., & Vardon, Derek Richard. Conversion of biomass to useful intermediates.  United States.

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                    Beckham, Gregg Tyler, Johnson, Christopher W., and Vardon, Derek Richard. Tue .  
        "Conversion of biomass to useful intermediates".  United States.  https://www.osti.gov/servlets/purl/1892914.

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@article{osti_1892914,

  title        = {Conversion of biomass to useful intermediates},

  author       = {Beckham, Gregg Tyler and Johnson, Christopher W. and Vardon, Derek Richard},

  abstractNote = {An aspect of the present disclosure is a microbial cell that includes a genetic modification resulting in the expression of a deficient form of an endogenous dioxygenase, and a gene encoding an exogenous dioxygenase and a promoter sequence, where the endogenous dioxygenase includes PcaH and PcaG, the exogenous dioxygenase includes LigA and LigB, the microbial cell is capable of growth utilizing at least one of a cellulose decomposition molecule or a lignin decomposition molecule, and the microbial cell is capable of producing 2-hydroxy-2H-pyran-4,6-dicarboxylic acid.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2022},

  month        = {5}

}

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