Engineered microbes for conversion of organic compounds to medium chain length alcohols and methods of use
Abstract
This disclosure provides a genetically-modified bacterium from the genus Pseudomonas that comprises an exogenous nucleic acid encoding an enoyl-CoA reductase and an exogenous nucleic acid encoding an acyl-CoA reductase that produces medium chain length alcohols. The disclosure further provides methods for producing medium chain alcohols using such genetically-modified bacterium. This disclosure provides a renewable, bio-based production platform for valuable mcl-alcohols that have a wide range of industrial applications. Current production of mcl-alcohols typically occurs through the hydrogenation of plant oils and waxes. This process leads to issues of deforestation and is largely unsustainable. Utilizing waste lignin streams as the carbon source provides a more sustainable feedstock that can be generated from plant waste like corn stover. Along with this, the use of lignin avoids competition with food resources as traditional starch and sugar feedstocks.
- Inventors:
- Issue Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1892911
- Patent Number(s):
- 11326151
- Application Number:
- 16/934,570
- Assignee:
- UT-Battelle, LLC (Oak Ridge, TN)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- AC05-00OR22725
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 07/21/2020
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Guss, Adam M., Elmore, Joshua R., and Huenemann, Jay D. Engineered microbes for conversion of organic compounds to medium chain length alcohols and methods of use. United States: N. p., 2022.
Web.
Guss, Adam M., Elmore, Joshua R., & Huenemann, Jay D. Engineered microbes for conversion of organic compounds to medium chain length alcohols and methods of use. United States.
Guss, Adam M., Elmore, Joshua R., and Huenemann, Jay D. Tue .
"Engineered microbes for conversion of organic compounds to medium chain length alcohols and methods of use". United States. https://www.osti.gov/servlets/purl/1892911.
@article{osti_1892911,
title = {Engineered microbes for conversion of organic compounds to medium chain length alcohols and methods of use},
author = {Guss, Adam M. and Elmore, Joshua R. and Huenemann, Jay D.},
abstractNote = {This disclosure provides a genetically-modified bacterium from the genus Pseudomonas that comprises an exogenous nucleic acid encoding an enoyl-CoA reductase and an exogenous nucleic acid encoding an acyl-CoA reductase that produces medium chain length alcohols. The disclosure further provides methods for producing medium chain alcohols using such genetically-modified bacterium. This disclosure provides a renewable, bio-based production platform for valuable mcl-alcohols that have a wide range of industrial applications. Current production of mcl-alcohols typically occurs through the hydrogenation of plant oils and waxes. This process leads to issues of deforestation and is largely unsustainable. Utilizing waste lignin streams as the carbon source provides a more sustainable feedstock that can be generated from plant waste like corn stover. Along with this, the use of lignin avoids competition with food resources as traditional starch and sugar feedstocks.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2022},
month = {5}
}
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