Media for culturing epithelial cells
Abstract
A method of growing primary human epithelial cells, in particular human epithelial cells using a basal formula containing individual (a) amino acids, (b) vitamins, (c) trace elements, and (d) other organics such as linoleic acid. The basal medium may be a mixture of amino acids, vitamins, and salts that constitute the basic media that is used to culture epithelial cells over a number of population doublings, e.g., over at least one week, while maintaining a normal phenotype and exerting low stress on the cultured cells, and maintaining lineage heterogeneity.
- Inventors:
- Issue Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE; National Institutes of Health (NIH)
- OSTI Identifier:
- 1892790
- Patent Number(s):
- 11293008
- Application Number:
- 15/762,517
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- AC02-05CH11231; AG040081
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 09/23/2016
- Country of Publication:
- United States
- Language:
- English
Citation Formats
LaBarge, Mark A., Stampfer, Martha R., and Garbe, James C. Media for culturing epithelial cells. United States: N. p., 2022.
Web.
LaBarge, Mark A., Stampfer, Martha R., & Garbe, James C. Media for culturing epithelial cells. United States.
LaBarge, Mark A., Stampfer, Martha R., and Garbe, James C. Tue .
"Media for culturing epithelial cells". United States. https://www.osti.gov/servlets/purl/1892790.
@article{osti_1892790,
title = {Media for culturing epithelial cells},
author = {LaBarge, Mark A. and Stampfer, Martha R. and Garbe, James C.},
abstractNote = {A method of growing primary human epithelial cells, in particular human epithelial cells using a basal formula containing individual (a) amino acids, (b) vitamins, (c) trace elements, and (d) other organics such as linoleic acid. The basal medium may be a mixture of amino acids, vitamins, and salts that constitute the basic media that is used to culture epithelial cells over a number of population doublings, e.g., over at least one week, while maintaining a normal phenotype and exerting low stress on the cultured cells, and maintaining lineage heterogeneity.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2022},
month = {4}
}
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