Volumetric micro-injector for capillary electrophoresis

Ha, Noel Seunghyun; Van Dam, R. Michael; Ly, Jimmy

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Title: Volumetric micro-injector for capillary electrophoresis

Abstract

A volumetric microfluidic injector for capillary electrophoresis (CE) for highly repeatable sample injection has been designed and built to eliminate known injection bias in hydrodynamic injection. A defined volume from 1-10 nL or 0.1-100 nL of sample is confined in a defined region of a micro-valve PDMS microfluidic injector chip and electrophoretic potential is applied to drive sample into a separation device such as an embedded fused silica capillary for separation and detection. Using a 75 μm ID capillary, the RSD of an absorbance peak area as low as 1.32% (n=11) is obtained. As a comparison, the time-dependent injection was tested using the same chip which resulted in an inferior repeatability.

Inventors:: Ha, Noel Seunghyun; Van Dam, R. Michael; Ly, Jimmy

Issue Date:: 2021-12-28

Research Org.:: Univ. of California, Oakland, CA (United States)

Sponsoring Org.:: USDOE; National Institutes of Health (NIH)

OSTI Identifier:: 1860210

Patent Number(s):: 11209390

Application Number:: 16/339,348

Assignee:: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA (Oakland, CA)

Patent Classifications (CPCs):: B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

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B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
B01L2300/0867 - Multiple inlets and one sample wells, e.g. mixing, dilution
B01L2400/0421 - electrophoretic flow
B01L2400/0487 - fluid pressure, pneumatics
B01L3/50273 - {characterised by the means or forces applied to move the fluids
B01L3/502738 - {characterised by integrated valves
B01L3/502753 - {characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N27/44743 - {Introducing samples}
G01N27/44791 - {Microapparatus
G01N27/453 - Cells therefor

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DOE Contract Number:: AG049918; SC0001249

Resource Type:: Patent

Resource Relation:: Patent File Date: 10/06/2017

Country of Publication:: United States

Language:: English

Citation Formats


                    Ha, Noel Seunghyun, Van Dam, R. Michael, and Ly, Jimmy. Volumetric micro-injector for capillary electrophoresis.  United States: N. p., 2021. 
        Web.

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                    Ha, Noel Seunghyun, Van Dam, R. Michael, & Ly, Jimmy. Volumetric micro-injector for capillary electrophoresis.  United States.

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                    Ha, Noel Seunghyun, Van Dam, R. Michael, and Ly, Jimmy. Tue .  
        "Volumetric micro-injector for capillary electrophoresis".  United States.  https://www.osti.gov/servlets/purl/1860210.

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@article{osti_1860210,

  title        = {Volumetric micro-injector for capillary electrophoresis},

  author       = {Ha, Noel Seunghyun and Van Dam, R. Michael and Ly, Jimmy},

  abstractNote = {A volumetric microfluidic injector for capillary electrophoresis (CE) for highly repeatable sample injection has been designed and built to eliminate known injection bias in hydrodynamic injection. A defined volume from 1-10 nL or 0.1-100 nL of sample is confined in a defined region of a micro-valve PDMS microfluidic injector chip and electrophoretic potential is applied to drive sample into a separation device such as an embedded fused silica capillary for separation and detection. Using a 75 μm ID capillary, the RSD of an absorbance peak area as low as 1.32% (n=11) is obtained. As a comparison, the time-dependent injection was tested using the same chip which resulted in an inferior repeatability.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2021},

  month        = {12}

}

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Similar Records

Title: Volumetric micro-injector for capillary electrophoresis

Abstract

Citation Formats

Integration of on-chip peristaltic pumps and injection valves with microchip electrophoresis and electrochemical detection journal, July 2010

Integrated multiplexed capillary electrophoresis system patent, May 2002

How to increase precision in capillary electrophoresis journal, January 2001

Pneumatic Microvalve-Based Hydrodynamic Sample Injection for High-Throughput, Quantitative Zone Electrophoresis in Capillaries journal, June 2014

Electrophoresis system patent-application, June 2016

Hydrodynamic injection with pneumatic valving for microchip electrophoresis with total analyte utilization journal, April 2011

Why not using capillary electrophoresis in drug analysis? journal, June 2006

Analytical instrument qualification in capillary electrophoresis: CE and CEC journal, June 2012

Development of fully automated quantitative capillary electrophoresis with high accuracy and repeatability: Development of fully automated quantitative capillary electrophoresis journal, September 2015

Sample preparation, processing and analysis systems patent-application, May 2015

Microfluidic Devices and Methods of Manufacture and Use patent-application, July 2015

Sample introduction techniques for microchip electrophoresis: A review journal, May 2012

High Efficiency and High Precision Microfluidic Devices and Methods patent-application, September 2008

Instrumentation design for hydrodynamic sample injection in microchip electrophoresis: A review: Microfluidics and Miniaturization journal, September 2012

Fluidic Devices with Diaphragm Valves patent-application, December 2010

Electrokinetic sample preconcentration and hydrodynamic sample injection for microchip electrophoresis using a pneumatic microvalve: Microfluidics and Miniaturization journal, November 2015

Integrated bio-analysis and sample preparation system patent-application, May 2005

Cassette for isolation, amplification and identification of DNA or protein and method of use patent-application, August 2005

Measuring system and method for autonomous measurement of an ion concentration with microchip capillary electrophoresis patent-application, August 2013

Microfluidic and Nanofluidic Devices, Systems, and Applications patent-application, February 2011

Design and Characterization of Poly(dimethylsiloxane)-Based Valves for Interfacing Continuous-Flow Sampling to Microchip Electrophoresis journal, February 2006

Integrated microfluidic device and methods patent, September 2015

Optimized Sample Injection Structures in Microfluidic Separations patent-application, January 2007

Integration of on-chip peristaltic pumps and injection valves with microchip electrophoresis and electrochemical detection
journal, July 2010

Integrated multiplexed capillary electrophoresis system
patent, May 2002

How to increase precision in capillary electrophoresis
journal, January 2001

Pneumatic Microvalve-Based Hydrodynamic Sample Injection for High-Throughput, Quantitative Zone Electrophoresis in Capillaries
journal, June 2014

Electrophoresis system
patent-application, June 2016

Hydrodynamic injection with pneumatic valving for microchip electrophoresis with total analyte utilization
journal, April 2011

Why not using capillary electrophoresis in drug analysis?
journal, June 2006

Analytical instrument qualification in capillary electrophoresis: CE and CEC
journal, June 2012

Development of fully automated quantitative capillary electrophoresis with high accuracy and repeatability: Development of fully automated quantitative capillary electrophoresis
journal, September 2015

Sample preparation, processing and analysis systems
patent-application, May 2015

Microfluidic Devices and Methods of Manufacture and Use
patent-application, July 2015

Sample introduction techniques for microchip electrophoresis: A review
journal, May 2012

High Efficiency and High Precision Microfluidic Devices and Methods
patent-application, September 2008

Instrumentation design for hydrodynamic sample injection in microchip electrophoresis: A review: Microfluidics and Miniaturization
journal, September 2012

Fluidic Devices with Diaphragm Valves
patent-application, December 2010

Electrokinetic sample preconcentration and hydrodynamic sample injection for microchip electrophoresis using a pneumatic microvalve: Microfluidics and Miniaturization
journal, November 2015

Integrated bio-analysis and sample preparation system
patent-application, May 2005

Cassette for isolation, amplification and identification of DNA or protein and method of use
patent-application, August 2005

Measuring system and method for autonomous measurement of an ion concentration with microchip capillary electrophoresis
patent-application, August 2013

Microfluidic and Nanofluidic Devices, Systems, and Applications
patent-application, February 2011

Design and Characterization of Poly(dimethylsiloxane)-Based Valves for Interfacing Continuous-Flow Sampling to Microchip Electrophoresis
journal, February 2006

Integrated microfluidic device and methods
patent, September 2015

Optimized Sample Injection Structures in Microfluidic Separations
patent-application, January 2007