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Title: Methods for producing microcompartments

Abstract

To produce a bacterial microcompartment shell, or a designed shell based on naturally occurring bacterial microcompartment shells in a new host organism, a synthetic operon is constructed that contains the desired shell protein genes and translation efficiency is controlled by host specific ribosomal binding sites. Proteins or other molecules can be encapsulated in the microcompartment shells by various methods described herein. The constructs can also be used to express self-assembling sheets comprised of shell proteins.

Inventors:
; ; ; ;
Issue Date:
Research Org.:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Univ. of California, Oakland, CA (United States)
Sponsoring Org.:
USDOE Advanced Research Projects Agency - Energy (ARPA-E); National Science Foundation (NSF)
OSTI Identifier:
1860163
Patent Number(s):
11198880
Application Number:
15/367,089
Assignee:
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA (Oakland, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
AC02-05CH11231; DE-0000200; MCB0851094; MCB1160614
Resource Type:
Patent
Resource Relation:
Patent File Date: 12/01/2016
Country of Publication:
United States
Language:
English

Citation Formats

Kerfeld, Cheryl A., Lassila, Jonathan K., Kinney, James N., Sutter, Markus, and Wilson, Steven C. Methods for producing microcompartments. United States: N. p., 2021. Web.
Kerfeld, Cheryl A., Lassila, Jonathan K., Kinney, James N., Sutter, Markus, & Wilson, Steven C. Methods for producing microcompartments. United States.
Kerfeld, Cheryl A., Lassila, Jonathan K., Kinney, James N., Sutter, Markus, and Wilson, Steven C. Tue . "Methods for producing microcompartments". United States. https://www.osti.gov/servlets/purl/1860163.
@article{osti_1860163,
title = {Methods for producing microcompartments},
author = {Kerfeld, Cheryl A. and Lassila, Jonathan K. and Kinney, James N. and Sutter, Markus and Wilson, Steven C.},
abstractNote = {To produce a bacterial microcompartment shell, or a designed shell based on naturally occurring bacterial microcompartment shells in a new host organism, a synthetic operon is constructed that contains the desired shell protein genes and translation efficiency is controlled by host specific ribosomal binding sites. Proteins or other molecules can be encapsulated in the microcompartment shells by various methods described herein. The constructs can also be used to express self-assembling sheets comprised of shell proteins.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2021},
month = {12}
}

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