Title: Increasing the content of long chain fatty acids in seed oil

Abstract

Provided are plants that express, or overexpress, a pPLAIIIδ protein. Constitutive or seed-specific expression of pPLAIIIδ protein in Arabidopsis increases seed oil content, the amount of C20 and C22 fatty acids, and the amount of C56, C58, and C60 triacylglycerols, effectively resulting in significantly higher oil yield per plant. Use of pPLAIIIδ is therefore an effective biotechnological tool to significantly increase plant yield, including oil, and the amount of high value long chain fatty acids in agricultural and horticultural crops, especially oilseed crops.

Inventors:

Wang, Xuemin; Li, Maoyin

Issue Date:

Tue Nov 09 00:00:00 EST 2021

Research Org.:

Donald Danforth Plant Science Center, St. Louis, MO (United States); Univ. of Missouri, Columbia, MO (United States)

Sponsoring Org.:

USDOE; National Science Foundation (NSF); National Natural Science Foundation of China (NSFC)

OSTI Identifier:

1860045

Patent Number(s):

11168331

Application Number:

16/362,346

Assignee:

Donald Danforth Plant Science Center (St. Louis, MO); The Curators of the University of Missouri (Columbia, MO)

Patent Classifications (CPCs):

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07C - ACYCLIC OR CARBOCYCLIC COMPOUNDS

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07C - ACYCLIC OR CARBOCYCLIC COMPOUNDS

C07C69/604 - Polycarboxylic acid esters, the acid moiety containing more than two carboxyl groups

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/8247 - {involving modified lipid metabolism, e.g. seed oil composition}
C12N9/20 - Triglyceride splitting, e.g. by means of lipase

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P7/6463 - {obtained from glyceride producing microorganisms, e.g. single cell oil}

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES

C12Y301/01 - Carboxylic ester hydrolases

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DOE Contract Number:  

30900787; DBI-0521587; EPS-0236913; MCB-0920663; MCB-0922879; SC0001295

Resource Type:

Patent

Resource Relation:

Patent File Date: 03/22/2019

Country of Publication:

United States

Language:

English