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Title: Engineering organisms resistant to viruses and horizontally transferred genetic elements

Abstract

Organisms resistant to horizontal gene transfer (HGT), and compositions and methods of use thereof are provided. The organisms are typically genomically recoded organisms (GRO), typically cells, having a genome in which at least one endogenous codon has been eliminated by reassignment of the codon to a synonymous or non-synonymous codon. The GRO typically include a recombinant expression construct for expression of at least one element of a ribosomal rescue pathway, typically lacking the eliminated codon. Typically, the disclosed cells are resistant to completed transfer and/or expression of a horizontally transferred genetic element (HTGE) from another organism compared a corresponding cell having a genome wherein the eliminated codon has not been eliminated. In some embodiments, the organism from which the HTGE is being transferred is a bacterium or a virus.

Inventors:
; ; ;
Issue Date:
Research Org.:
Yale Univ., New Haven, CT (United States)
Sponsoring Org.:
USDOE; Defense Advanced Research Projects Agency (DARPA); National Institutes of Health (NIH)
OSTI Identifier:
1859963
Patent Number(s):
11149280
Application Number:
17/107,555
Assignee:
Yale University (New Haven, CT)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FG02-02ER63445; GM007223; GM007499; GM117230; GM125951; HR0011-15-C-0091; N66001-12-C-4211
Resource Type:
Patent
Resource Relation:
Patent File Date: 11/30/2020
Country of Publication:
United States
Language:
English

Citation Formats

Isaacs, Farren J., Ma, Natalie, Rinehart, Jesse, and Hemez, Colin. Engineering organisms resistant to viruses and horizontally transferred genetic elements. United States: N. p., 2021. Web.
Isaacs, Farren J., Ma, Natalie, Rinehart, Jesse, & Hemez, Colin. Engineering organisms resistant to viruses and horizontally transferred genetic elements. United States.
Isaacs, Farren J., Ma, Natalie, Rinehart, Jesse, and Hemez, Colin. Tue . "Engineering organisms resistant to viruses and horizontally transferred genetic elements". United States. https://www.osti.gov/servlets/purl/1859963.
@article{osti_1859963,
title = {Engineering organisms resistant to viruses and horizontally transferred genetic elements},
author = {Isaacs, Farren J. and Ma, Natalie and Rinehart, Jesse and Hemez, Colin},
abstractNote = {Organisms resistant to horizontal gene transfer (HGT), and compositions and methods of use thereof are provided. The organisms are typically genomically recoded organisms (GRO), typically cells, having a genome in which at least one endogenous codon has been eliminated by reassignment of the codon to a synonymous or non-synonymous codon. The GRO typically include a recombinant expression construct for expression of at least one element of a ribosomal rescue pathway, typically lacking the eliminated codon. Typically, the disclosed cells are resistant to completed transfer and/or expression of a horizontally transferred genetic element (HTGE) from another organism compared a corresponding cell having a genome wherein the eliminated codon has not been eliminated. In some embodiments, the organism from which the HTGE is being transferred is a bacterium or a virus.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2021},
month = {10}
}

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