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Title: Atmospheric-pressure ionization and fragmentation of molecules for structural elucidation

Abstract

A solution-cathode glow discharge mass spectrometry (SCGD-MS) apparatus comprises a SCGD source and a mass spectrometer. The SCGD source may comprise conductive rods, a power source, and a capillary. A method for ionizing an analyte comprises flowing an electrically conductive liquid onto a conductive rod, applying an electric potential to a second conductive rod such that a plasma discharge forms between the first conductive rod and the electrically conductive liquid to produce ions, and separating the ions in a mass spectrometer. The analyte may be a polypeptide that may be contacted with trypsin. The analyte may be a solid, liquid, gas, chemical complex, or ion in solution. The method may comprise sequencing the polypeptide.

Inventors:
; ; ;
Issue Date:
Research Org.:
Indiana Univ., Indianapolis, IN (United States); Kent State Univ., OH (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1840498
Patent Number(s):
11133170
Application Number:
16/997,061
Assignee:
Indiana University Research and Technology Corporation (Indianapolis, IN); Kent State University (Kent, OH)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
H - ELECTRICITY H01 - BASIC ELECTRIC ELEMENTS H01J - ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
DOE Contract Number:  
FG02-98ER14890
Resource Type:
Patent
Resource Relation:
Patent File Date: 08/19/2020
Country of Publication:
United States
Language:
English

Citation Formats

Shelley, Jacob T., Williams, Kelsey L., Hieftje, Gary M., and Schwartz, Andrew J. Atmospheric-pressure ionization and fragmentation of molecules for structural elucidation. United States: N. p., 2021. Web.
Shelley, Jacob T., Williams, Kelsey L., Hieftje, Gary M., & Schwartz, Andrew J. Atmospheric-pressure ionization and fragmentation of molecules for structural elucidation. United States.
Shelley, Jacob T., Williams, Kelsey L., Hieftje, Gary M., and Schwartz, Andrew J. Tue . "Atmospheric-pressure ionization and fragmentation of molecules for structural elucidation". United States. https://www.osti.gov/servlets/purl/1840498.
@article{osti_1840498,
title = {Atmospheric-pressure ionization and fragmentation of molecules for structural elucidation},
author = {Shelley, Jacob T. and Williams, Kelsey L. and Hieftje, Gary M. and Schwartz, Andrew J.},
abstractNote = {A solution-cathode glow discharge mass spectrometry (SCGD-MS) apparatus comprises a SCGD source and a mass spectrometer. The SCGD source may comprise conductive rods, a power source, and a capillary. A method for ionizing an analyte comprises flowing an electrically conductive liquid onto a conductive rod, applying an electric potential to a second conductive rod such that a plasma discharge forms between the first conductive rod and the electrically conductive liquid to produce ions, and separating the ions in a mass spectrometer. The analyte may be a polypeptide that may be contacted with trypsin. The analyte may be a solid, liquid, gas, chemical complex, or ion in solution. The method may comprise sequencing the polypeptide.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2021},
month = {9}
}

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patent-application, November 2015


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