Gene editing through microfluidic delivery
Abstract
Gene editing can be performed by introducing gene-editing components into a cell by mechanical cell disruption. Related apparatus, systems, techniques, and articles are also described. The methods and systems of the invention solve the problem of intracellular delivery of gene editing components and gene editing complexes to target cells. The results described herein indicate that delivery of gene editing components, e.g., protein, ribonucleic acid (RNA), and deoxyribonucleic acid (DNA), by mechanical disruption of cell membranes leads to successful gene editing. Because intracellular delivery of gene editing materials is a current challenge, the methods provide a robust mechanism to engineer target cells without the use of potentially harmful viral vectors or electric fields.
- Inventors:
- Issue Date:
- Research Org.:
- Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States); Harvard Univ., Cambridge, MA (United States)
- Sponsoring Org.:
- USDOE; National Institutes of Health (NIH)
- OSTI Identifier:
- 1840473
- Patent Number(s):
- 11125739
- Application Number:
- 15/542,892
- Assignee:
- Massachusetts Institute of Technology (Cambridge, MA); President and Fellows of Harvard College (Cambridge, MA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12M - APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- FG02-02ER63445; R01 GM101420
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 01/12/2016
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Sharei, Armon R., Lajoie, Marc J., Jensen, Klavs F., and Langer, Robert S. Gene editing through microfluidic delivery. United States: N. p., 2021.
Web.
Sharei, Armon R., Lajoie, Marc J., Jensen, Klavs F., & Langer, Robert S. Gene editing through microfluidic delivery. United States.
Sharei, Armon R., Lajoie, Marc J., Jensen, Klavs F., and Langer, Robert S. Tue .
"Gene editing through microfluidic delivery". United States. https://www.osti.gov/servlets/purl/1840473.
@article{osti_1840473,
title = {Gene editing through microfluidic delivery},
author = {Sharei, Armon R. and Lajoie, Marc J. and Jensen, Klavs F. and Langer, Robert S.},
abstractNote = {Gene editing can be performed by introducing gene-editing components into a cell by mechanical cell disruption. Related apparatus, systems, techniques, and articles are also described. The methods and systems of the invention solve the problem of intracellular delivery of gene editing components and gene editing complexes to target cells. The results described herein indicate that delivery of gene editing components, e.g., protein, ribonucleic acid (RNA), and deoxyribonucleic acid (DNA), by mechanical disruption of cell membranes leads to successful gene editing. Because intracellular delivery of gene editing materials is a current challenge, the methods provide a robust mechanism to engineer target cells without the use of potentially harmful viral vectors or electric fields.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2021},
month = {9}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Cellular delivery and activation of polypeptide-nucleic acid complexes
patent-application, September 2004
- Dalby, Brian; Bennett, Robert P.
- US Patent Application 10/755082; 20040176282
Antigen specific immunosuppression by dendritic cell therapy
patent-application, December 2008
- Lee, Brendan; Seiler, Michael; Cerullo, Vincenzo
- US Patent Application 12/156179; 20080311140
Method for achieving site specific integration of exogenous DNA delivered by non-biological means to plant cells
patent, June 2002
- Hansen, Genevieve; Chilton, MaryDell; Suttie, Janet L.
- US Patent Document 6,410,329
Disruption and field enabled delivery of compounds and compositions into cells
patent, January 2020
- Ding, Xiaoyun; Sharei, Armon R.; Langer, Robert S.
- US Patent Document 10,526,573
Modified and fusion enhanced erythrocytes, cells and uses thereof
patent-application, April 2011
- Glaser, Larry F.
- US Patent Application 12/314341; 20110091973
CRISPR-Cas9 delivery to hard-to-transfect cells via membrane deformation
journal, August 2015
- Han, Xin; Liu, Zongbin; Jo, Myeong chan
- Science Advances, Vol. 1, Issue 7
Spatially selective reagent delivery into cancer cells using a two-layer microfluidic culture system
journal, September 2012
- Liu, Yan; Butler, W. Boyd; Pappas, Dimitri
- Analytica Chimica Acta, Vol. 743
Multi-step microfluidic device for studying cancer metastasis
journal, January 2007
- Chaw, K. C.; Manimaran, M.; Tay, E. H.
- Lab on a Chip, Vol. 7, Issue 8
Methods and devices for cell culturing and organ assist systems
patent, May 2003
- Toner, Mehmet; Yarmush, Martin L.; Balis, Ulysses J.
- US Patent Document 6,562,616
Shear-induced intracellular loading of cells with molecules by controlled microfluidics
journal, January 2008
- Hallow, Daniel M.; Seeger, Richard A.; Kamaev, Pavel P.
- Biotechnology and Bioengineering, Vol. 99, Issue 4
Methods and Apparatus for the Manipulation of Particle Suspensions and Testing Thereof
patent-application, October 2007
- Ionescu-Zanetti, Cristian; Khine, Michelle; Schwartz, Michael
- US Patent Application 11/690831; 20070243523
Selective delivery of material to cells
patent, December 2020
- Sharei, Armon R.; Adalsteinsson, Viktor A.; Cho, Nahyun
- US Patent Document 10,870,112
Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform
journal, November 2013
- Sharei, Armon; Cho, Nahyun; Mao, Shirley
- Journal of Visualized Experiments, Issue 81
Microfluidic Chip for Capturing Cells and Preparing Method Thereof
patent-application, July 2015
- Liu, Kan; Wang, Shengxiang; Zhang, Nangang
- US Patent Application 14/418122; 20150196913
Intracellular Delivery
patent-application, March 2019
- Sharei, Armon R.; Adamo, Andrea; Langer, Robert S.
- US Patent Application 16/141107; 20190093073
Biochip for High-Throughput Screening of Circulating Tumor Cells
patent-application, December 2010
- Chiu, Daniel T.; Kuo, Jason S.; Shelby, J. Patrick
- US Patent Application 12/665575; 20100323388
1743-P: Engineering Erythrocytes with the SQZ Cell Therapy Platform to Enhance Immunotolerance
journal, June 2019
- Ramakrishnan, Amritha; Lee, Thomas; Moore, Lindsay J.
- Diabetes, Vol. 68, Issue Supplement_1
Molecular Imaging in Tracking Tumor-Specific Cytotoxic T Lymphocytes (CTLs)
journal, January 2014
- Liu, Zhiyi; Li, Zheng
- Theranostics, Vol. 4, Issue 10
The Use of Therapeutic Peptides to Target and to Kill Cancer Cells
journal, June 2012
- J. Boohaker, R.; W. Lee, M.; Vishnubhotla, P.
- Current Medicinal Chemistry, Vol. 19, Issue 22
Biological targeting compositions and methods of using the same
patent, July 2012
- Hyde, Roderick A.; Ishikawa, Muriel Y.; Jung, Edward K. Y.
- US Patent Document 8,211,656
Delivery of Materials to Anucleate Cells
patent-application, July 2018
- Sharei, Armon R.; Jensen, Klavs F.; Abshire, James Robbins
- US Patent Application 15/865901; 20180201889
Compostition and therapeutic anti-tumour vaccine
patent, June 2016
- Godfrin, Yann; Banz, Alice
- US Patent Document 9,364,504
Direct visualization at the single-cell level of electrically mediated gene delivery
journal, January 2002
- Golzio, Muriel; Teissié, Justin; Rols, Marie-Pierre
- Proceedings of the National Academy of Sciences, Vol. 99, Issue 3
Direct visualization at the single-cell level of siRNA electrotransfer into cancer cells
journal, June 2011
- Paganin-Gioanni, A.; Bellard, E.; Escoffre, J. M.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 26
Composition to Induce Specific Immune Tolerance
patent-application, August 2012
- Godfrin, Yann; Banz, Alice
- US Patent Application 13/503955; 20120207745
Measurement of Material Properties and Related Methods and Compositions
patent-application, March 2012
- Suresh, Subra; Han, Jongyoon; Bow, Hansen
- US Patent Application 13/053118; 20120064505
RNA-Guided Human Genome Engineering via Cas9
journal, January 2013
- Mali, P.; Yang, L.; Esvelt, K. M.
- Science, Vol. 339, Issue 6121, p. 823-826
Methods and Systems for Continuous Flow Cell Lysis in a Microfluidic Device
patent-application, July 2015
- White, Ian M.; Burke, Jeffrey; Pandit, Kunal
- US Patent Application 14/586576; 20150184127
Self-Assembling Complexes of Quantum Dots and scFv Antibodies for Cancer Cell Targeting and Imaging
journal, October 2012
- Zdobnova, Tatiana A.; Stremovskiy, Oleg A.; Lebedenko, Ekaterina N.
- PLoS ONE, Vol. 7, Issue 10
Studies on anticancer activities of antimicrobial peptides
journal, February 2008
- Hoskin, David W.; Ramamoorthy, Ayyalusamy
- Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1778, Issue 2
Sex Differences in Plasmacytoid Dendritic Cell Levels of IRF5 Drive Higher IFN- Production in Women
journal, October 2015
- Griesbeck, M.; Ziegler, S.; Laffont, S.
- The Journal of Immunology, Vol. 195, Issue 11
Methods and compositions for enhanced immunological therapy and targeting of gram-positive bacteria
patent, April 2015
- Nowinski, Robert C.; Fischetti, Vincent; Raz, Assaf
- US Patent Document 9,005,579
Apparatus and process for determining the deformability of the red corpuscles in the blood
patent, May 1989
- Hanss, deceased, Maxime F.; Guillet, R.; Vassauk, D.
- US Patent Document 4,835,457
Systemic treatment with CAR-engineered T cells against PSCA delays subcutaneous tumor growth and prolongs survival of mice
journal, January 2014
- Hillerdal, Victoria; Ramachandran, Mohanraj; Leja, Justyna
- BMC Cancer, Vol. 14, Issue 1
Methods and apparatus for the isolation and enrichment of circulating tumor cells
patent, March 2014
- Chiu, Daniel T.; Kuo, Jason S.
- US Patent Document 8,669,044
Red blood cells as innovative antigen carrier to induce specific immune tolerance
journal, February 2013
- Cremel, Magali; Guérin, Nathalie; Horand, Françoise
- International Journal of Pharmaceutics, Vol. 443, Issue 1-2
Delivery of Biomolecules to Immune Cells
patent-application, May 2018
- Sharei, Armon R.; Mao, Shirley; Hartoularos, George
- US Patent Application 15/523142; 20180142198
Self Replicating RNA Molecules and Uses Thereof
patent-application, December 2011
- Geall, Andrew; Hekele, Armin; Mandl, Christian
- US Patent Application 12/831252; 20110300205
Engineered binding to erythrocytes induces immunological tolerance to
E. coli
asparaginase
journal, July 2015
- Lorentz, Kristen M.; Kontos, Stephan; Diaceri, Giacomo
- Science Advances, Vol. 1, Issue 6
Intracellular delivery
patent, June 2020
- Sharei, Armon R.; Adamo, Andrea; Langer, Robert S.
- US Patent Document 10,696,944
Intracellular Delivery
patent-application, September 2014
- Sharei, Armon R.; Adamos, Andrea; Langer, Robert S.
- US Patent Application 14/352354; 20140287509
High-Throughput Cargo Delivery Into Live Cells Using Photothermal Platforms
patent-application, January 2016
- Wu, Yi-Chen; Wu, Ting-Hsiang S.; Chiou, Pei-Yu E.
- US Patent Application 14/771478; 20160017340
Protein Identification and Analysis Tools on the ExPASy Server
book, January 2005
- Gasteiger, Elisabeth; Hoogland, Christine; Gattiker, Alexandre
- The Proteomics Protocols Handbook
Tolerance Induction in Experimental Autoimmune Encephalomyelitis Using Non-myeloablative Hematopoietic Gene Therapy With Autoantigen
journal, May 2009
- Eixarch, Herena; Espejo, Carmen; Gómez, Alba
- Molecular Therapy, Vol. 17, Issue 5
A brief overview of electroporation pulse strength–duration space: A region where additional intracellular effects are expected
journal, October 2012
- Weaver, James C.; Smith, Kyle C.; Esser, Axel T.
- Bioelectrochemistry, Vol. 87
Methods of Switching the Phenotype of T Cells by Transgenic Lineage Factor Foxp3
patent-application, August 2010
- Betz, Alexander G.; Andersen, Kristian G.
- US Patent Application 12/678724; 20100203068
Cellular adaptation to mechanical stress: role of integrins, Rho, cytoskeletal tension and mechanosensitive ion channels
journal, February 2006
- Matthews, Benjamin D.; Overby, Darryl R.; Mannix, Robert
- Journal of Cell Science, Vol. 119, Issue 3
Cell culture models using rat primary alveolar type I cells
journal, October 2011
- Downs, Charles A.; Montgomery, David W.; Merkle, Carrie J.
- Pulmonary Pharmacology & Therapeutics, Vol. 24, Issue 5
Genetically Modified Animals and Methods for Making the Same
patent-application, August 2012
- Fahrenkrug, Scott C.; Carlson, Daniel F.
- US Patent Application 13/404662; 20120222143
Intercellular Delivery and Transfection Methods and Devices
patent-application, September 2014
- Meacham, John Mark; Durvasula, Kiran; Fedorov, Andrei G.
- US Patent Application 14/205948; 20140273229
Loading of cells with antigens by electroporation
patent-application, June 2006
- Liu, Linda N.; Weiss, Jonathan M.
- US Patent Application 11/219307; 20060134067
Intracellular Delivery of Biomolecules Mediated by a Surface With Pores
patent-application, December 2019
- Gilbert, Jonathan B.; Frew, Kirubel; Bernstein, Howard
- US Patent Application 15/757312; 20190382796
CRISPR-Cas systems and methods for altering expression of gene products
patent, April 2014
- Zhang, Feng
- US Patent Document 8,697,359
Compound delivery using high-pressure impulse transients
patent, August 1997
- Flotte, Thomas J.; Doukas, Apostolos G.; McAuliffe, Daniel J.
- US Patent Document 5,658,892
Highly selective biomechanical separation of cancer cells from leukocytes using microfluidic ratchets and hydrodynamic concentrator
journal, May 2013
- Lin, Bill K.; McFaul, Sarah M.; Jin, Chao
- Biomicrofluidics, Vol. 7, Issue 3
Adjuvant incorporation into antigen carrying cells: compositions and methods
patent, April 2001
- Ravindranath, Mepur H.; Morton, Donald L.
- US Patent Document 6,218,166
Intraerythrocytic administration of a synthetic Plasmodium antigen elicits antibody response in mice, without carrier molecules or adjuvants
journal, December 1990
- Esposito, F.; Santroni, A.; Habluetzel, A.
- International Journal for Parasitology, Vol. 20, Issue 8
Synthetic antigen presenting matrix
patent, October 2002
- Cai, Zeling; Sprent, Jonathan; Brunmark, Anders
- US Patent Document 6,461,867
Patch clamping on plane glass—fabrication of hourglass aperture and high-yield ion channel recording
journal, January 2009
- Chen, Chang-Yu; Tu, Ting-Yuan; Chen, Chang-Hung
- Lab on a Chip, Vol. 9, Issue 16
What is (Still not) Known of the Mechanism by Which Electroporation Mediates Gene Transfer and Expression in Cells and Tissues
journal, November 2008
- Escoffre, Jean-Michel; Portet, Thomas; Wasungu, Luc
- Molecular Biotechnology, Vol. 41, Issue 3
Sample probes and methods for sampling intracellular material
patent, February 2016
- Bernick, Kristin Briana; Sampas, Nicholas M.
- US Patent Document 9,255,245
Oral vaccine comprising antigen surface-associated with red blood cells
patent, July 1997
- Pang, Gerald; Clancy, Robert
- US Patent Document 5,643,577
Methods and Compositions for Immunomodulation
patent-application, January 2017
- Mata-Fink, Jordi; Round, JOhn; Afeyan, Noubar B.
- US Patent Application 15/301046; 20170020926
Method of and device for measuring elastic and di-electric properties of the diaphragm of living cells
patent, October 1977
- Coster, Hans Gerard Leonard; Pilwat, Gunter; Zimmermann, Ulrich
- US Patent Document 4,055,799
Blood filter and apparatus for hemorheological measurement
patent, June 1991
- Sato, Kazuo; Kikuchi, Yuji; Ohki, Hiroshi
- US Patent Document 5,023,054
Shear Effects on Stability of DNA Complexes in the Presence of Serum
journal, September 2017
- Wen, Hao; Yu, Qiuhong; Yin, Yudan
- Biomacromolecules, Vol. 18, Issue 10
Plasma membrane recovery kinetics of a microfluidic intracellular delivery platform
journal, January 2014
- Sharei, Armon; Poceviciute, Roberta; Jackson, Emily L.
- Integr. Biol., Vol. 6, Issue 4
Method for combined parallel agent delivery and electroporation for cell structures and thereof
patent-application, February 2005
- Ormar, Owe; Karlsson, Mattias; Nolkrantz, Kerstin
- US Patent Application 10/496214; 20050026283
Microfluidic, Label-Free Enrichment of Prostate Cancer Cells in Blood Based on Acoustophoresis
journal, August 2012
- Augustsson, Per; Magnusson, Cecilia; Nordin, Maria
- Analytical Chemistry, Vol. 84, Issue 18
Analysis of in vitro Transfection by Sonoporation Using Cationic and Neutral Microbubbles
journal, November 2010
- Tlaxca, Jose L.; Anderson, Christopher R.; Klibanov, Alexander L.
- Ultrasound in Medicine & Biology, Vol. 36, Issue 11
Electrosonic cell manipulation device and method of use thereof
patent, April 2010
- Fedorov, Andrei G.; Degertekin, Fahrettin Levent
- US Patent Document 7,704,743
Microfluidic squeezing for intracellular antigen loading in polyclonal B-cells as cellular vaccines
journal, May 2015
- Szeto, Gregory Lee; Van Egeren, Debra; Worku, Hermoon
- Scientific Reports, Vol. 5, Issue 1
Nonendocytic Delivery of Functional Engineered Nanoparticles into the Cytoplasm of Live Cells Using a Novel, High-Throughput Microfluidic Device
journal, February 2012
- Lee, Jungmin; Sharei, Armon; Sim, Woo Young
- Nano Letters, Vol. 12, Issue 12
Stabilization of biopolymer microgels formed by electrostatic complexation: Influence of enzyme (laccase) cross-linking on pH, thermal, and mechanical stability
journal, December 2015
- Azarikia, Fatemeh; Wu, Bi-cheng; Abbasi, Soleiman
- Food Research International, Vol. 78
Composition and therapeutic anti-tumour vaccine
patent, April 2018
- Godfrin, Yann; Banz, Alice
- US Patent Document 9,950,049
Microfluidic systems incorporating varied channel dimensions
patent, December 1998
- Kopf-Sill, Anne R.; Parce, John Wallace
- US Patent Document 5,842,787
Selective delivery of material to cells
patent, November 2018
- Sharei, Armon R.; Adalsteinsson, Viktor A.; Cho, Nahyun
- US Patent Document 10,124,336
High-throughput nuclear delivery and rapid expression of DNA via mechanical and electrical cell-membrane disruption
journal, March 2017
- Ding, Xiaoyun; Stewart, Martin P.; Sharei, Armon
- Nature Biomedical Engineering, Vol. 1, Issue 3
Compositions and Methods Related to Multimodal Therapeutic Cell Systems for Cancer Indications
patent-application, March 2018
- Kahvejian, Avak; Mata-Fink, Jordi; Deans, Robert J.
- US Patent Application 15/716141; 20180085402
Controlled fluid transport microfabricated polymeric substrates
patent, December 2000
- Parce, John Wallace; Knapp, Michael R.; Chow, Calvin Y. H.
- US Patent Document 6,156,181
Innovative approach in Pompe disease therapy: Induction of immune tolerance by antigen-encapsulated red blood cells
journal, August 2015
- Cremel, Magali; Guerin, Nathalie; Campello, Gaelle
- International Journal of Pharmaceutics, Vol. 491, Issue 1-2
Glucose transporter expression on the plasma membrane of resting and activated white blood cells
journal, April 2007
- Maratou, E.; Dimitriadis, G.; Kollias, A.
- European Journal of Clinical Investigation, Vol. 37, Issue 4
Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells
journal, April 2015
- Sharei, Armon; Trifonova, Radiana; Jhunjhunwala, Siddharth
- PLOS ONE, Vol. 10, Issue 4
System for High Throughput Measurement of Mechanical Properties of Cells
patent-application, November 2009
- Adamo, Andrea; Dokov, Rangle P.; Jensen, Klava F.
- US Patent Application 12/436814; 20090280518
Erythrocytes as Carrier for Drugs, Enzymes and Peptides
journal, April 2012
- Ravilla, Srinu
- journal of applied pharmaceutical science, Vol. 2, Issue 4
Nanomechanical analysis of cells from cancer patients
journal, December 2007
- Cross, Sarah E.; Jin, Yu-Sheng; Rao, Jianyu
- Nature Nanotechnology, Vol. 2, Issue 12
Transfected human dendritic cells to induce antitumor immunity
journal, September 2000
- Rughetti, A.; Biffoni, M.; Sabbatucci, M.
- Gene Therapy, Vol. 7, Issue 17
Mammalian artificial chromosomes and methods of using same
patent, October 2000
- Scheffler, Immo E.
- US Patent Document 6,133,503
Selective Delivery of Material to Cells
patent-application, July 2016
- Sharei, Armon R.; Adalsteinsson, Viktor A.; Cho, Nahyun
- US Patent Application 14/912001; 20160193605
Sample Probes and Methods for Sampling Intracellular Material
patent-application, January 2014
- Bernick, Kristin Briana; Sampas, Nicholas M.
- US Patent Application 13/541478; 20140011226
Rapid screening of biologically active nucleases and isolation of nuclease-modified cells
patent-application, January 2011
- Holmes, Michael C.; Li, Tianjian
- US Patent Application 12/803552; 20110014616
Rational design of a split-Cas9 enzyme complex
journal, February 2015
- Wright, Addison V.; Sternberg, Samuel H.; Taylor, David W.
- Proceedings of the National Academy of Sciences, Vol. 112, Issue 10
Opsonin-enhanced cells, and methods of modulating an immune response to an antigen
patent, September 1999
- Segal, Andrew H.
- US Patent Document 5,951,976
Pulsed Laser Triggered High Speed Microfluidic Switch and Applications in Fluorescent Activated Cell Sorting
patent-application, February 2011
- Chiou, Pei-YU; Wu, Ting-Asiang
- US Patent Application 12/852320; 20110030808
Devices for Separating Cells and Methods of Using Them
patent-application, May 2012
- Li, Mo Huang; Ying, Jackie Y.; Wei, Jiashen
- US Patent Application 13/257972; 20120107925
Protein-Coupled Red Blood Cell Compositions and Methods of Their Use
patent-application, November 2017
- Jajosky, Ryan; Pacholczyk, Rafal; Singh, Gurmukh
- US Patent Application 15/596586; 20170326213
Methods tip assemblies and kits for introducing material into cells
patent, April 2015
- Diefenbach, Thomas J.
- US Patent Document 9,017,991
Intracellular Delivery of Biomolecules to Induce Tolerance
patent-application, April 2019
- Gilbert, Jonathan B.; Bu, Wang; Loughhead, Scott
- US Patent Application 16/098404; 20190111082
Movement and Selection of Micro-Objects in a Microfluidic Apparatus
patent-application, July 2016
- Breinlinger, Keith J.; Hobbs, Eric D.; Malleo, Daniele
- US Patent Application 14/965721; 20160199837
Oral tolerance using allogeneic platelets in ITP
patent-application, July 2003
- Kasha, Jr., John R.
- US Patent Application 10/045189; 20030133922
Methods and apparatus for the isolation and enrichment of circulating tumor cells
patent, August 2011
- Chiu, Daniel T.; Kuo, Jason S.
- US Patent Document 7,993,821
Microfluidic systems and methods
patent, October 2015
- Wheeler, Matthew B.; Kim, Dongshin; Beebe, David J.
- US Patent Document 9,157,550
A vector-free microfluidic platform for intracellular delivery
journal, January 2013
- Sharei, A.; Zoldan, J.; Adamo, A.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 6
Scientific Basis for the Use of Hypotonic Solutions with Ultrasonic Liposuction
journal, March 2006
- Song, Angela Y.; Bennett, Jennifer M.; Marra, Kacey G.
- Aesthetic Plastic Surgery, Vol. 30, Issue 2
Selective Delivery of Material to Cells
patent-application, January 2019
- Sharei, Armon R.; Adalsteinsson, Viktor A.; Cho, Nahyun
- US Patent Application 16/145865; 20190030536
Memory of tolerance and induction of regulatory T cells by erythrocyte-targeted antigens
journal, October 2015
- Grimm, Alizée J.; Kontos, Stephan; Diaceri, Giacomo
- Scientific Reports, Vol. 5, Issue 1
165 Activating antigen carriers generated with microfluidics cell squeezing drive effective anti-tumor responses
conference, November 2020
- Blagovic, Katarina; Ramakrishnan, Amritha; Sharei, Armon
- Regular and young investigator award abstracts
Techniques for Transfecting Protoplasts
patent-application, January 2013
- Bundock, Paul; Fierens-Onstenk, Bernarda Gerharda Johanna; Lhuissier, Franck
- US Patent Application 13/517375; 20130023051
Single cell optical transfection
journal, January 2010
- Stevenson, David J.; Gunn-Moore, Frank J.; Campbell, Paul
- Journal of The Royal Society Interface, Vol. 7, Issue 47
Microfluidic device for single cell targeted operations
patent-application, October 2007
- Adamo, Andrea; Jensen, Klavs Flemming; Adamo, Luigi
- US Patent Application 11/788646; 20070249038
Microfluidic-Enabled Intracellular Delivery of Membrane Impermeable Inhibitors to Study Target Engagement in Human Primary Cells
journal, November 2017
- Li, Jing; Wang, Bu; Juba, Brian M.
- ACS Chemical Biology, Vol. 12, Issue 12
Size-Selective Microcavity Array for Rapid and Efficient Detection of Circulating Tumor Cells
journal, June 2010
- Hosokawa, Masahito; Hayata, Taishi; Fukuda, Yorikane
- Analytical Chemistry, Vol. 82, Issue 15
Hydrodynamic stretching of single cells for large population mechanical phenotyping
journal, April 2012
- Gossett, D. R.; Tse, H. T. K.; Lee, S. A.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 20
Disruption and Field Enabled Delivery of Compounds and Compositions into Cells
patent-application, January 2018
- Ding, Xiaoyun; Sharei, Armon R.; Langer, Robert S.
- US Patent Application 15/526517; 20180016539
Method and Apparatus for Microfluidic Injection
patent-application, August 2009
- Adamo, Andrea; Adamo, Luigi; Jensen, Klavs F.
- US Patent Application 12/370146; 20090209039
Delivery technologies for genome editing
journal, March 2017
- Yin, Hao; Kauffman, Kevin J.; Anderson, Daniel G.
- Nature Reviews Drug Discovery, Vol. 16, Issue 6
Microfluidics-Based Assessment of Cell Deformability
journal, July 2012
- Adamo, Andrea; Sharei, Armon; Adamo, Luigi
- Analytical Chemistry, Vol. 84, Issue 15
Electrosonic ejector microarray for drug and gene delivery
journal, November 2007
- Zarnitsyn, Vladimir G.; Meacham, J. Mark; Varady, Mark J.
- Biomedical Microdevices, Vol. 10, Issue 2
Microengineered Platforms for Cell Mechanobiology
journal, August 2009
- Kim, Deok-Ho; Wong, Pak Kin; Park, Jungyul
- Annual Review of Biomedical Engineering, Vol. 11, Issue 1
Tolerogenic Dendritic Cells
journal, April 2003
- Steinman, Ralph M.; Hawiger, Daniel; Nussenzweig, Michel C.
- Annual Review of Immunology, Vol. 21, Issue 1
Compact Biocompatible Quantum Dots via RAFT-Mediated Synthesis of Imidazole-Based Random Copolymer Ligand
journal, January 2010
- Liu, Wenhao; Greytak, Andrew B.; Lee, Jungmin
- Journal of the American Chemical Society, Vol. 132, Issue 2
Human erythrocytes as drug carriers: Loading efficiency and side effects of hypotonic dialysis, chlorpromazine treatment and fusion with liposomes
journal, September 2013
- Favretto, M. E.; Cluitmans, J. C. A.; Bosman, G. J. C. G. M.
- Journal of Controlled Release, Vol. 170, Issue 3
Intracellular Delivery of Complexes
patent-application, January 2019
- Ditommaso, Tia; Bernstein, Howard; Sharei, Armon R.
- US Patent Application 16/068631; 20190017072
Intracellular Delivery
patent-application, September 2020
- Sharei, Armon R.; Adamo, Andrea; Langer, Robert S.
- US Patent Application 16/818021; 20200277566
Gene Editing Through Microfluidic Delivery
patent-application, January 2018
- Sharei, Armon R.; Lajoie, Marc; Jensen, Klavs F.
- US Patent Application 15/542892; 20180003696
Blood Fluidity Measurement Apparatus and Blood Fluidity Measurement Method
patent-application, September 2010
- Ichitani, Shuji; Toyama, Osamu
- US Patent Application 12/744604; 20100249621
Mechanical Stiffness Grades Metastatic Potential in Patient Tumor Cells and in Cancer Cell Lines
journal, June 2011
- Swaminathan, V.; Mythreye, K.; O'Brien, E. T.
- Cancer Research, Vol. 71, Issue 15
Tolerogenic dendritic cells: cytokine modulation comes of age
journal, September 2006
- Rutella, Sergio; Danese, Silvio; Leone, Giuseppe
- Blood, Vol. 108, Issue 5
Expansion of immature, nucleated red blood cells by transient low-dose methotrexate immune tolerance induction in mice
journal, December 2020
- Tran, J. Q.; Grover, D.; Zhang, M.
- Clinical and Experimental Immunology, Vol. 203, Issue 3
Methods and Compositions for Enhanced Immunological Therapy and Targeting of Gram-Positive Bacteria
patent-application, February 2013
- Nowinski, Robert C.; Fischetti, Vincent A.; Raz, Assaf
- US Patent Application 13/520601; 20130045211
Method for electro-permeabilization of individual cellular and organellar structures and use thereof
patent, September 2006
- Orwar, Owe; Lundqvist, Anders; Eriksson, Peter
- US Patent Document 7,109,034
Microfluidic systems incorporating varied channel dimensions
patent, February 2001
- Kopf-Sill, Anne R.; Parce, John Wallace
- US Patent Document 6,186,660
Tumor Growth Control Using Red Blood Cells as the Antigen Delivery System and Poly(I
journal, June 2012
- Banz, Alice; Cremel, Magali; Mouvant, Amélie
- Journal of Immunotherapy, Vol. 35, Issue 5
Complexation between milk proteins and polysaccharides via electrostatic interaction: principles and applications – a review
journal, March 2008
- Ye, Aiqian
- International Journal of Food Science & Technology, Vol. 43, Issue 3
Intracellular Delivery of Biomolecules to Cells Comprising a Cell Wall
patent-application, August 2018
- Sharei, Armon R.; Bernstein, Howard; Gilbert, Jonathan B.
- US Patent Application 15/757316; 20180245089
What is the total number of protein molecules per cell volume? A call to rethink some published values: Insights & Perspectives
journal, September 2013
- Milo, Ron
- BioEssays, Vol. 35, Issue 12
T-cell-inducing vaccines - what’s the future: T-cell vaccines
journal, December 2011
- Gilbert, Sarah C.
- Immunology, Vol. 135, Issue 1
Biochip for High-Throughput Screening of Circulating Tumor Cells
patent-application, December 2008
- Chiu, Daniel T.; Kuo, Jason S.; Shelby, J. Patrick
- US Patent Application 11/766053; 20080318324
Measurement of Wall Shearing Stress in the Boundary Layer by Means of an Evaporating Liquid Film
journal, September 1956
- Murphy, James S.; Smith, A. M. O.
- Journal of Applied Physics, Vol. 27, Issue 9
A microfluidic model for single-cell capillary obstruction by Plasmodium falciparum-infected erythrocytes
journal, November 2003
- Shelby, J. P.; White, J.; Ganesan, K.
- Proceedings of the National Academy of Sciences, Vol. 100, Issue 25
Generating binary states using a microfluidic channel
patent, September 2014
- Glick, Casey G.
- US Patent Document 8,844,570
Cell Culturing Method Using Biomechanical Stimulation Loading And System Therefor
patent-application, January 2008
- Nakata, Ken
- US Patent Application 11/629619; 20080026465
The Cpf1 CRISPR-Cas protein expands genome-editing tools
journal, November 2015
- Fagerlund, Robert D.; Staals, Raymond H. J.; Fineran, Peter C.
- Genome Biology, Vol. 16, Issue 1
Monovalent, reduced-size quantum dots for imaging receptors on living cells
journal, April 2008
- Howarth, Mark; Liu, Wenhao; Puthenveetil, Sujiet
- Nature Methods, Vol. 5, Issue 5
In vitro and ex vivo strategies for intracellular delivery
journal, October 2016
- Stewart, Martin P.; Sharei, Armon; Ding, Xiaoyun
- Nature, Vol. 538, Issue 7624
Cas9 gRNA engineering for genome editing, activation and repression
journal, September 2015
- Kiani, Samira; Chavez, Alejandro; Tuttle, Marcelle
- Nature Methods, Vol. 12, Issue 11
Algal Transformation Systems, Compositions and Methods
patent-application, March 2013
- MacMillan, Trevor Wayne
- US Patent Application 13/594259; 20130065314
Electrosonic Cell Manipulation Device and Method of Use Thereof
patent-application, October 2006
- Fedorov, Andrei G.; Degertekin, Fahrettin L.
- US Patent Application 11/277662; 20060223185
System for locating cells and for cellular analysis
patent-application, June 2006
- Miles, Robin R.; Crawford, Jackie; Christian, Allen T.
- US Patent Application 11/283454; 20060134772
Extracellular potential measuring device and method for fabricating the same
patent-application, October 2004
- Nakatani, Masaya; Oka, Hiroaki; Emoto, Fumiaki
- US Patent Application 10/485644; 20040197898
Erythrocyte-mediated delivery of phenylalanine ammonia lyase for the treatment of phenylketonuria in BTBR-Pahenu2 mice
journal, November 2014
- Rossi, Luigia; Pierigè, Francesca; Carducci, Claudia
- Journal of Controlled Release, Vol. 194