Title: Nanoscale biochemical sample preparation and analysis

Abstract

Provided herein are methods and systems for biochemical analysis, including compositions and methods for processing and analysis of small cell populations and biological samples (e.g., a robotically controlled chip-based nanodroplet platform). In particular aspects, the methods described herein can reduce total processing volumes from conventional volumes to nanoliter volumes within a single reactor vessel (e.g., within a single droplet reactor) while minimizing losses, such as due to sample evaporation.

Inventors:

Kelly, Ryan T.; Zhu, Ying; Smith, Richard D.

Issue Date:

2021-09-21

Research Org.:

Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Org.:

USDOE; National Institutes of Health (NIH)

OSTI Identifier:

1840463

Patent Number(s):

11123732

Application Number:

15/897,022

Assignee:

Battelle Memorial Institute (Richland, WA)

Patent Classifications (CPCs):

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61B - DIAGNOSIS

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY

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A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61B - DIAGNOSIS

A61B10/02 - Instruments for taking cell samples or for biopsy {

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY

B01J19/0046 - {Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides
B01J2219/00317 - Microwell devices, i.e. having large numbers of wells
B01J2219/00367 - capillary
B01J2219/00619 - using hydrophilic or hydrophobic regions
B01J2219/00621 - by physical means, e.g. trenches, raised areas
B01J2219/00659 - Two-dimensional arrays
B01J2219/00702 - Processes involving means for analysing and characterising the products
B01J2219/00725 - Peptides
B01J2219/00743 - Cells

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

B01L2200/142 - Preventing evaporation
B01L2300/0819 - Microarrays
B01L2300/0822 - Slides
B01L2300/0887 - Laminated structure
B01L3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware
B01L3/502753 - {characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
B01L3/50853 - {with covers or lids}
B01L3/5088 - {confining liquids at a location by surface tension, e.g. virtual wells on plates, wires

C - CHEMISTRY C12 - BIOCHEMISTRY C12M - APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY

C12M23/58 - {Reaction vessels connected in series or in parallel
C12M41/00 - {Means for regulation, monitoring, measurement or control, e.g. flow regulation

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q1/6881 - for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes

C - CHEMISTRY C40 - COMBINATORIAL TECHNOLOGY C40B - COMBINATORIAL CHEMISTRY

C40B60/04 - Integrated apparatus specially adapted for both screening libraries and identifying library members

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N1/2813 - {Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
G01N1/30 - Staining
G01N1/312 - {for samples mounted on planar substrates}
G01N1/34 - Purifying
G01N2001/284 - {using local activation of adhesive, i.e. Laser Capture Microdissection}
G01N2030/8831 - {involving peptides or proteins}
G01N30/88 - Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
G01N33/4833 - {of solid biological material, e.g. tissue samples, cell cultures
G01N33/68 - involving proteins, peptides or amino acids {
G01N33/6848 - {Methods of protein analysis involving mass spectrometry}

G - PHYSICS G02 - OPTICS G02B - OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS

G02B21/32 - Micromanipulators structurally combined with microscopes

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DOE Contract Number:  

AC05-76RL01830; P41GM103493; R21EB020976

Resource Type:

Patent

Resource Relation:

Patent File Date: 02/14/2018

Country of Publication:

United States

Language:

English