DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Nanoscale biochemical sample preparation and analysis

Abstract

Provided herein are methods and systems for biochemical analysis, including compositions and methods for processing and analysis of small cell populations and biological samples (e.g., a robotically controlled chip-based nanodroplet platform). In particular aspects, the methods described herein can reduce total processing volumes from conventional volumes to nanoliter volumes within a single reactor vessel (e.g., within a single droplet reactor) while minimizing losses, such as due to sample evaporation.

Inventors:
; ;
Issue Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE; National Institutes of Health (NIH)
OSTI Identifier:
1840463
Patent Number(s):
11123732
Application Number:
15/897,022
Assignee:
Battelle Memorial Institute (Richland, WA)
Patent Classifications (CPCs):
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61B - DIAGNOSIS
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY
DOE Contract Number:  
AC05-76RL01830; P41GM103493; R21EB020976
Resource Type:
Patent
Resource Relation:
Patent File Date: 02/14/2018
Country of Publication:
United States
Language:
English

Citation Formats

Kelly, Ryan T., Zhu, Ying, and Smith, Richard D. Nanoscale biochemical sample preparation and analysis. United States: N. p., 2021. Web.
Kelly, Ryan T., Zhu, Ying, & Smith, Richard D. Nanoscale biochemical sample preparation and analysis. United States.
Kelly, Ryan T., Zhu, Ying, and Smith, Richard D. Tue . "Nanoscale biochemical sample preparation and analysis". United States. https://www.osti.gov/servlets/purl/1840463.
@article{osti_1840463,
title = {Nanoscale biochemical sample preparation and analysis},
author = {Kelly, Ryan T. and Zhu, Ying and Smith, Richard D.},
abstractNote = {Provided herein are methods and systems for biochemical analysis, including compositions and methods for processing and analysis of small cell populations and biological samples (e.g., a robotically controlled chip-based nanodroplet platform). In particular aspects, the methods described herein can reduce total processing volumes from conventional volumes to nanoliter volumes within a single reactor vessel (e.g., within a single droplet reactor) while minimizing losses, such as due to sample evaporation.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2021},
month = {9}
}

Works referenced in this record:

Apparatus and method for analyzing a metabolite profile
patent-application, January 2007


The pancreas in human type 1 diabetes
journal, May 2010


Molecular imaging of proteins in tissues by mass spectrometry
journal, September 2008


Single-cell sequencing-based technologies will revolutionize whole-organism science
journal, July 2013


The MaxQuant computational platform for mass spectrometry-based shotgun proteomics
journal, October 2016


Quantitative Studies on Cell Proteins in Suspension Cultures
journal, January 1970


NIH Image to ImageJ: 25 years of image analysis
journal, June 2012


Ultrasensitive and Fast Bottom-up Analysis of Femtogram Amounts of Complex Proteome Digests
journal, October 2013


Nanodroplet processing platform for deep and quantitative proteome profiling of 10–100 mammalian cells
journal, February 2018


Getting intimate with trypsin, the leading protease in proteomics: TRYPSIN IN PROTEOMICS
journal, June 2013


A “Proteomic Ruler” for Protein Copy Number and Concentration Estimation without Spike-in Standards
journal, September 2014


Methods for processing biological tissue
patent-application, July 2006


High-throughput spatial mapping of single-cell RNA-seq data to tissue of origin
journal, April 2015


Quantification of islet size and architecture
journal, March 2012


Quantitative proteomic analysis of single pancreatic islets
journal, October 2009


SNaPP: Simplified Nanoproteomics Platform for Reproducible Global Proteomic Analysis of Nanogram Protein Quantities
journal, March 2016


Spatially-Resolved Proteomics: Rapid Quantitative Analysis of Laser Capture Microdissected Alveolar Tissue Samples
journal, December 2016


Development and Evaluation of a Micro- and Nanoscale Proteomic Sample Preparation Method
journal, December 2005


Enzyme-Friendly, Mass Spectrometry-Compatible Surfactant for In-Solution Enzymatic Digestion of Proteins
journal, November 2003


Proteomic profiling of human islets collected from frozen pancreata using laser capture microdissection
journal, January 2017


Nanoliter-Scale Protein Crystallization and Screening with a Microfluidic Droplet Robot
journal, May 2014


Massively Parallel Single-Cell RNA-Seq for Marker-Free Decomposition of Tissues into Cell Types
journal, February 2014


An Integrated Platform for Isolation, Processing, and Mass Spectrometry-based Proteomic Profiling of Rare Cells in Whole Blood
journal, March 2015


High-throughput microfluidic single-cell RT-qPCR
journal, August 2011


The importance of using the optimal plasticware and glassware in studies involving peptides
journal, July 2011


High Throughput Miniaturized Assay System and Methods
patent-application, August 2014


Development of Mass Spectrometry-Based Shotgun Method for Proteome Analysis of 500 to 5000 Cancer Cells
journal, March 2010


Heterogeneity and Lobularity of Pancreatic Pathology in Type 1 Diabetes during the Prediabetic Phase
journal, July 2015


High Recovery FASP Applied to the Proteomic Analysis of Microdissected Formalin Fixed Paraffin Embedded Cancer Tissues Retrieves Known Colon Cancer Markers
journal, July 2011


Obtaining Analytes for a Tissue Specimen
patent-application, May 2013


High-Precision Dispensing of Nanoliter Biofluids on Glass Pedestal Arrays for Ultrasensitive Biomolecule Detection
journal, April 2016


Single-Cell Mass Spectrometry for Discovery Proteomics: Quantifying Translational Cell Heterogeneity in the 16-Cell Frog ( Xenopus ) Embryo
journal, January 2016


Ultrasensitive Proteomics Using High-Efficiency On-Line Micro-SPE-NanoLC-NanoESI MS and MS/MS
journal, January 2004


BoxPlotR: a web tool for generation of box plots
journal, January 2014


The Perseus computational platform for comprehensive analysis of (prote)omics data
journal, June 2016


Simple and Integrated Spintip-Based Technology Applied for Deep Proteome Profiling
journal, April 2016


Isobaric Labeling-Based Relative Quantification in Shotgun Proteomics
journal, November 2014


Ultrasensitive and Quantitative Analyses from Combined Separations−Mass Spectrometry for the Characterization of Proteomes
journal, April 2004


New Mass-Spectrometry-Compatible Degradable Surfactant for Tissue Proteomics
journal, January 2015


Quantitative analysis of cell composition and purity of human pancreatic islet preparations
journal, August 2010


Islet cell hyperexpression of HLA class I antigens: a defining feature in type 1 diabetes
journal, August 2016