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Title: Host cells and methods for producing toluene biochemically

Abstract

The present invention provides for a genetically modified host cell comprising a first polypeptide comprising a sequence having at least 70% amino acid sequence identity with a phenylacetate decarboxylase, and having an enzymatic activity to decarboxylate a phenylacetic acid into a toluene and a carbon dioxide, and a second polypeptide comprising a sequence having at least 70% amino acid sequence identity with a phenylacetate decarboxylase activating enzyme, and having an enzymatic activity to cleave a S-adenosylmethionine (SAM) to form a methionine and a 5′-deoxyadenosyl radical.

Inventors:
; ;
Issue Date:
Research Org.:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1840306
Patent Number(s):
11078499
Application Number:
16/286,411
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Patent
Resource Relation:
Patent File Date: 02/26/2019
Country of Publication:
United States
Language:
English

Citation Formats

Beller, Harry R., Rodrigues, Andria V., and Zargar, Kamrun. Host cells and methods for producing toluene biochemically. United States: N. p., 2021. Web.
Beller, Harry R., Rodrigues, Andria V., & Zargar, Kamrun. Host cells and methods for producing toluene biochemically. United States.
Beller, Harry R., Rodrigues, Andria V., and Zargar, Kamrun. Tue . "Host cells and methods for producing toluene biochemically". United States. https://www.osti.gov/servlets/purl/1840306.
@article{osti_1840306,
title = {Host cells and methods for producing toluene biochemically},
author = {Beller, Harry R. and Rodrigues, Andria V. and Zargar, Kamrun},
abstractNote = {The present invention provides for a genetically modified host cell comprising a first polypeptide comprising a sequence having at least 70% amino acid sequence identity with a phenylacetate decarboxylase, and having an enzymatic activity to decarboxylate a phenylacetic acid into a toluene and a carbon dioxide, and a second polypeptide comprising a sequence having at least 70% amino acid sequence identity with a phenylacetate decarboxylase activating enzyme, and having an enzymatic activity to cleave a S-adenosylmethionine (SAM) to form a methionine and a 5′-deoxyadenosyl radical.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2021},
month = {8}
}

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