Polypeptide and yeast cell compositions and methods of using the same

Hittinger, Chris Todd; Baker, EmilyClare Patricia

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A - human necessities
A01 - agriculture
A21 - baking
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D10 - indexing scheme associated with sublasses of section d, relating to textiles
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Title: Polypeptide and yeast cell compositions and methods of using the same

Abstract

Polypeptides comprising maltose/maltotriose transporters are provided. Additionally, polynucleotides, DNA constructs, and vectors encoding a maltose/maltotriose transporter, or yeast cells harboring such polynucleotides are provided. The yeast cell may be a Saccharomyces eubayanus cell modified to increase the expression or transport activity of a maltose/maltotriose transporter at the plasma membrane of the cell. Further, methods are provided for making a fermentation product by culturing any one of the yeast cells described herein with a fermentable substrate. Finally, methods are provided to select for and isolate maltotriose-utilizing strains of Saccharomyces eubayanus.

Inventors:: Hittinger, Chris Todd; Baker, EmilyClare Patricia

Issue Date:: 2021-06-08

Research Org.:: Univ. of Wisconsin, Madison, WI (United States)

Sponsoring Org.:: USDOE; US Dept. of Agriculture (USDA); National Science Foundation (NSF)

OSTI Identifier:: 1824048

Patent Number(s):: 11028402

Application Number:: 16/378,106

Assignee:: Wisconsin Alumni Research Foundation (Madison, WI)

Patent Classifications (CPCs):: C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C - CHEMISTRY C12 - BIOCHEMISTRY C12C - BEER

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C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C07K14/395 - from Saccharomyces

C - CHEMISTRY C12 - BIOCHEMISTRY C12C - BEER
C12C11/00 - Fermentation processes for beer

C - CHEMISTRY C12 - BIOCHEMISTRY C12G - WINE
C12G1/0203 - {by microbiological or enzymatic treatment}
C12G3/02 - by fermentation

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N1/16 - Yeasts
C12N15/81 - for yeasts

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P7/00 - Preparation of oxygen-containing organic compounds

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DOE Contract Number:: 17-CRHF-0-6055; DEB1253634; FC02-07ER64494

Resource Type:: Patent

Resource Relation:: Patent File Date: 04/08/2019

Country of Publication:: United States

Language:: English

Citation Formats


                    Hittinger, Chris Todd, and Baker, EmilyClare Patricia. Polypeptide and yeast cell compositions and methods of using the same.  United States: N. p., 2021. 
        Web.

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                    Hittinger, Chris Todd, & Baker, EmilyClare Patricia. Polypeptide and yeast cell compositions and methods of using the same.  United States.

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                    Hittinger, Chris Todd, and Baker, EmilyClare Patricia. Tue .  
        "Polypeptide and yeast cell compositions and methods of using the same".  United States.  https://www.osti.gov/servlets/purl/1824048.

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@article{osti_1824048,

  title        = {Polypeptide and yeast cell compositions and methods of using the same},

  author       = {Hittinger, Chris Todd and Baker, EmilyClare Patricia},

  abstractNote = {Polypeptides comprising maltose/maltotriose transporters are provided. Additionally, polynucleotides, DNA constructs, and vectors encoding a maltose/maltotriose transporter, or yeast cells harboring such polynucleotides are provided. The yeast cell may be a Saccharomyces eubayanus cell modified to increase the expression or transport activity of a maltose/maltotriose transporter at the plasma membrane of the cell. Further, methods are provided for making a fermentation product by culturing any one of the yeast cells described herein with a fermentable substrate. Finally, methods are provided to select for and isolate maltotriose-utilizing strains of Saccharomyces eubayanus.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2021},

  month        = {6}

}

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Works referenced in this record:

Genetically Engineered Yeast Cell Having Increased Nadph Production, Method of Increasing Nadph Level in Yeast Cell, Method of Preparing Yeast Cell, and Method of Producing Lactate Using Yeast Cell
patent-application, November 2016

Jung, Yukyung; Cho, Kwangmyung; Kim, Jinha
US Patent Application 15/066775; 20160340698
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20160340698

Glucose-induced inactivation/degradation-resistant transporter gene and use thereof
patent, November 2011

Hatanaka, Haruyo; Omura, Fumihiko
US Patent Document 8,053,224
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/8053224

Constructs and Methods for Genome Editing and Genetic Engineering of Fungi and Protists
patent-application, June 2018

Hittinger, Christopher Todd; Alexander, William Gerald
US Patent Application 15/878698; 20180155732
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20180155732

Constructs and Methods for Genome Editing and Genetic Engineering of Fungi and Protists
patent-application, February 2016

Hittinger, Christopher Todd; Alexander, William Gerald
US Patent Application 14/826566; 20160046952
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20160046952

Synthetic Yeast Cells and Methods of Making and Using the Same
patent-application, May 2013

Alexander, William Gerald; Navarro, David Peris; Hittinger, Christopher Todd
US Patent Application 15/805950; 20180127784
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20180127784

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Polypeptides comprising maltose/maltotriose transporters are provided. Additionally, polynucleotides, DNA constructs, and vectors encoding a maltose/maltotriose transporter, or yeast cells harboring such polynucleotides are provided. The yeast cell may be a Saccharomyces eubayanus cell modified to increase the expression or transport activity of a maltose/maltotriose transporter at the plasma membrane of the cell. Further, methods are provided for making a fermentation product by culturing any one of the yeast cells described herein with a fermentable substrate. Finally, methods are provided to select for and isolate maltotriose-utilizing strains of Saccharomyces eubayanus.
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Similar Records

Title: Polypeptide and yeast cell compositions and methods of using the same

Abstract

Citation Formats

Genetically Engineered Yeast Cell Having Increased Nadph Production, Method of Increasing Nadph Level in Yeast Cell, Method of Preparing Yeast Cell, and Method of Producing Lactate Using Yeast Cell patent-application, November 2016

Glucose-induced inactivation/degradation-resistant transporter gene and use thereof patent, November 2011

Constructs and Methods for Genome Editing and Genetic Engineering of Fungi and Protists patent-application, June 2018

Constructs and Methods for Genome Editing and Genetic Engineering of Fungi and Protists patent-application, February 2016

Synthetic Yeast Cells and Methods of Making and Using the Same patent-application, May 2013

Genetically Engineered Yeast Cell Having Increased Nadph Production, Method of Increasing Nadph Level in Yeast Cell, Method of Preparing Yeast Cell, and Method of Producing Lactate Using Yeast Cell
patent-application, November 2016

Glucose-induced inactivation/degradation-resistant transporter gene and use thereof
patent, November 2011

Constructs and Methods for Genome Editing and Genetic Engineering of Fungi and Protists
patent-application, June 2018

Constructs and Methods for Genome Editing and Genetic Engineering of Fungi and Protists
patent-application, February 2016

Synthetic Yeast Cells and Methods of Making and Using the Same
patent-application, May 2013