Polypeptide and yeast cell compositions and methods of using the same

Hittinger, Chris Todd; Baker, EmilyClare Patricia

Title: Polypeptide and yeast cell compositions and methods of using the same

Abstract

Polypeptides comprising maltose/maltotriose transporters are provided. Additionally, polynucleotides, DNA constructs, and vectors encoding a maltose/maltotriose transporter, or yeast cells harboring such polynucleotides are provided. The yeast cell may be a Saccharomyces eubayanus cell modified to increase the expression or transport activity of a maltose/maltotriose transporter at the plasma membrane of the cell. Further, methods are provided for making a fermentation product by culturing any one of the yeast cells described herein with a fermentable substrate. Finally, methods are provided to select for and isolate maltotriose-utilizing strains of Saccharomyces eubayanus.

Inventors:: Hittinger, Chris Todd; Baker, EmilyClare Patricia

Issue Date:: 2021-06-08

Research Org.:: Univ. of Wisconsin, Madison, WI (United States)

Sponsoring Org.:: USDOE; US Dept. of Agriculture (USDA); National Science Foundation (NSF)

OSTI Identifier:: 1824048

Patent Number(s):: 11028402

Application Number:: 16/378,106

Assignee:: Wisconsin Alumni Research Foundation (Madison, WI)

Patent Classifications (CPCs):: C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C - CHEMISTRY C12 - BIOCHEMISTRY C12C - BEER

Show more

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C07K14/395 - from Saccharomyces

C - CHEMISTRY C12 - BIOCHEMISTRY C12C - BEER
C12C11/00 - Fermentation processes for beer

C - CHEMISTRY C12 - BIOCHEMISTRY C12G - WINE
C12G1/0203 - {by microbiological or enzymatic treatment}
C12G3/02 - by fermentation

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N1/16 - Yeasts
C12N15/81 - for yeasts

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P7/00 - Preparation of oxygen-containing organic compounds

Show less

DOE Contract Number:: 17-CRHF-0-6055; DEB1253634; FC02-07ER64494

Resource Type:: Patent

Resource Relation:: Patent File Date: 04/08/2019

Country of Publication:: United States

Language:: English

Citation Formats


                    Hittinger, Chris Todd, and Baker, EmilyClare Patricia. Polypeptide and yeast cell compositions and methods of using the same.  United States: N. p., 2021. 
        Web.

Copy to clipboard


                    Hittinger, Chris Todd, & Baker, EmilyClare Patricia. Polypeptide and yeast cell compositions and methods of using the same.  United States.

Copy to clipboard


                    Hittinger, Chris Todd, and Baker, EmilyClare Patricia. Tue .  
        "Polypeptide and yeast cell compositions and methods of using the same".  United States.  https://www.osti.gov/servlets/purl/1824048.

Copy to clipboard


                    
@article{osti_1824048,

  title        = {Polypeptide and yeast cell compositions and methods of using the same},

  author       = {Hittinger, Chris Todd and Baker, EmilyClare Patricia},

  abstractNote = {Polypeptides comprising maltose/maltotriose transporters are provided. Additionally, polynucleotides, DNA constructs, and vectors encoding a maltose/maltotriose transporter, or yeast cells harboring such polynucleotides are provided. The yeast cell may be a Saccharomyces eubayanus cell modified to increase the expression or transport activity of a maltose/maltotriose transporter at the plasma membrane of the cell. Further, methods are provided for making a fermentation product by culturing any one of the yeast cells described herein with a fermentable substrate. Finally, methods are provided to select for and isolate maltotriose-utilizing strains of Saccharomyces eubayanus.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2021},

  month        = {6}

}

Copy to clipboard

Patent:

Save / Share:

Export Metadata

Save to My Library

Works referenced in this record:

Genetically Engineered Yeast Cell Having Increased Nadph Production, Method of Increasing Nadph Level in Yeast Cell, Method of Preparing Yeast Cell, and Method of Producing Lactate Using Yeast Cell
patent-application, November 2016

Jung, Yukyung; Cho, Kwangmyung; Kim, Jinha
US Patent Application 15/066775; 20160340698
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20160340698

Glucose-induced inactivation/degradation-resistant transporter gene and use thereof
patent, November 2011

Hatanaka, Haruyo; Omura, Fumihiko
US Patent Document 8,053,224
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/8053224

Constructs and Methods for Genome Editing and Genetic Engineering of Fungi and Protists
patent-application, June 2018

Hittinger, Christopher Todd; Alexander, William Gerald
US Patent Application 15/878698; 20180155732
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20180155732

Constructs and Methods for Genome Editing and Genetic Engineering of Fungi and Protists
patent-application, February 2016

Hittinger, Christopher Todd; Alexander, William Gerald
US Patent Application 14/826566; 20160046952
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20160046952

Synthetic Yeast Cells and Methods of Making and Using the Same
patent-application, May 2013

Alexander, William Gerald; Navarro, David Peris; Hittinger, Christopher Todd
US Patent Application 15/805950; 20180127784
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20180127784

Similar Records in DOE Patents and OSTI.GOV collections:

Compositions comprising a GH61 polypeptide having cellulolytic enhancing activity and a liquor and method of using thereof

Patent Quinlan, Jason; Xu, Feng; Sweeney, Matt; ...

The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and a liquor. The present invention also relates to methods of using the compositions.
Full Text Available
Host cells and methods using a repressor polypeptide and an inducible promoter for gene expression

Patent Ruegg, Thomas L.

The present invention provides for a system comprising (a) a first nucleic acid comprising a nucleotide sequence encoding a nucleotide sequence of interest operatively linked to a promoter comprising a repressor polypeptide binding site, and (b) a second nucleic acid comprising a nucleotide sequence encoding a repressor polypeptide having at least 70% amino acid identity with EilR, SmvR, KmrR, RcdA, or QacR; wherein expression of the nucleotide sequence of interest from the promoter is induced by the presence of a hydrophobic inducer, such as a hydrophobic cation inducer, such as a triarylmethane, acridine, phenazine, phenothiazine, or xanthene.
Full Text Available
Host cells and methods using a repressor polypeptide and an inducible promoter for gene expression

Patent Ruegg, Thomas L.

The present invention provides for a system comprising (a) a first nucleic acid comprising a nucleotide sequence encoding a nucleotide sequence of interest operatively linked to a promoter comprising a repressor polypeptide binding site, and (b) a second nucleic acid comprising a nucleotide sequence encoding a repressor polypeptide having at least 70% amino acid identity with EilR, SmvR, KmrR, RcdA, or QacR; wherein expression of the nucleotide sequence of interest from the promoter is induced by the presence of a hydrophobic inducer, such as a hydrophobic cation inducer, such as a triarylmethane, acridine, phenazine, phenothiazine, or xanthene.
Full Text Available
Boron-based dual imaging probes, compositions and methods for rapid aqueous F-18 labeling, and imaging methods using same

Patent Li, Zibo; Gabbai, Francois P.; Conti, Peter S.; ...

A composition useful as a PET and/or fluorescence imaging probe a compound a compound of Formula I, including salts, hydrates and solvates thereof: ##STR00001## wherein R.sub.1-R.sub.7 may be independently selected from hydrogen, halogen, hydroxy, alkoxy, nitro, substituted and unsubstituted amino, cycloalkyl, carboxy, carboxylic acids and esters thereof, cyano, haloalkyl, aryl, X is selected from the group consisting of C and N; and A is selected of hydrogen, halogen, hydroxy, alkoxy, nitro, substituted and unsubstituted amino, alkyl, cycloalkyl, carboxy, carboxylic acids and esters thereof, cyano, haloalkyl, aryl, including phenyl and aminophenyl, and heteroaryl.
Full Text Available
Ferroelectric fluoride compositions and methods of making and using same

Patent Halasyamani, P Shiv; Chang, Hong-Young

A method for synthesis of a ferroelectric material characterized by the general formula A.sub.xB.sub.yF.sub.z where A is an alkaline earth metal, B is transition metal or a main group metal, x and y each range from about 1 to about 5, and z ranges from about 1 to about 20 comprising contacting an alkaline earth metal fluoride, a difluorometal compound and a fluoroorganic acid in a medium to form a reaction mixture; and subjecting the reaction mixture to conditions suitable for hydrothermal crystal growth.
Full Text Available

Similar Records

Title: Polypeptide and yeast cell compositions and methods of using the same

Abstract

Citation Formats

Genetically Engineered Yeast Cell Having Increased Nadph Production, Method of Increasing Nadph Level in Yeast Cell, Method of Preparing Yeast Cell, and Method of Producing Lactate Using Yeast Cell patent-application, November 2016

Glucose-induced inactivation/degradation-resistant transporter gene and use thereof patent, November 2011

Constructs and Methods for Genome Editing and Genetic Engineering of Fungi and Protists patent-application, June 2018

Constructs and Methods for Genome Editing and Genetic Engineering of Fungi and Protists patent-application, February 2016

Synthetic Yeast Cells and Methods of Making and Using the Same patent-application, May 2013

Genetically Engineered Yeast Cell Having Increased Nadph Production, Method of Increasing Nadph Level in Yeast Cell, Method of Preparing Yeast Cell, and Method of Producing Lactate Using Yeast Cell
patent-application, November 2016

Glucose-induced inactivation/degradation-resistant transporter gene and use thereof
patent, November 2011

Constructs and Methods for Genome Editing and Genetic Engineering of Fungi and Protists
patent-application, June 2018

Constructs and Methods for Genome Editing and Genetic Engineering of Fungi and Protists
patent-application, February 2016

Synthetic Yeast Cells and Methods of Making and Using the Same
patent-application, May 2013