Production of polyhydroxy alkanoates with a defined composition from an unrelated carbon source
Abstract
Cells and methods for producing polyhydroxyalkanoates. The cells comprise one or more recombinant genes selected from an R-specific enoyl-CoA hydratase gene, a PHA polymerase gene, a thioesterase gene, and an acyl-CoA-synthetase gene. The cells further have one or more genes functionally deleted. The functionally deleted genes include such genes as an enoyl-CoA hydratase gene, a 3-hydroxyacyl-CoA dehydrogenase, and a 3-ketoacyl-CoA thiolase gene. The recombinant cells are capable of using producing polyhydroxyalkanoates with a high proportion of monomers having the same carbon length from non-lipid substrates, such as carbohydrates.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of Wisconsin, Madison, WI (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1823870
- Patent Number(s):
- 10982240
- Application Number:
- 15/915,964
- Assignee:
- Wisconsin Alumni Research Foundation (Madison, WI)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- FC02-07ER64494
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 03/08/2018
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Pfleger, Brian Frederick, and Agnew, Daniel E. Production of polyhydroxy alkanoates with a defined composition from an unrelated carbon source. United States: N. p., 2021.
Web.
Pfleger, Brian Frederick, & Agnew, Daniel E. Production of polyhydroxy alkanoates with a defined composition from an unrelated carbon source. United States.
Pfleger, Brian Frederick, and Agnew, Daniel E. Tue .
"Production of polyhydroxy alkanoates with a defined composition from an unrelated carbon source". United States. https://www.osti.gov/servlets/purl/1823870.
@article{osti_1823870,
title = {Production of polyhydroxy alkanoates with a defined composition from an unrelated carbon source},
author = {Pfleger, Brian Frederick and Agnew, Daniel E.},
abstractNote = {Cells and methods for producing polyhydroxyalkanoates. The cells comprise one or more recombinant genes selected from an R-specific enoyl-CoA hydratase gene, a PHA polymerase gene, a thioesterase gene, and an acyl-CoA-synthetase gene. The cells further have one or more genes functionally deleted. The functionally deleted genes include such genes as an enoyl-CoA hydratase gene, a 3-hydroxyacyl-CoA dehydrogenase, and a 3-ketoacyl-CoA thiolase gene. The recombinant cells are capable of using producing polyhydroxyalkanoates with a high proportion of monomers having the same carbon length from non-lipid substrates, such as carbohydrates.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2021},
month = {4}
}
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