DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Innovative nanopore sequencing technology including a self-assembled porous membrane

Abstract

Methods and apparatus for long read, label-free, optical nanopore long chain molecule sequencing. In general, the present disclosure describes a novel sequencing technology based on the integration of nanochannels to deliver single long-chain molecules with widely spaced (>wavelength), ˜1-nm aperture “tortuous” nanopores that slow translocation sufficiently to provide massively parallel, single base resolution using optical techniques. A novel, directed self-assembly nanofabrication scheme using simple colloidal nanoparticles is used to form the nanopore arrays atop nanochannels that unfold the long chain molecules. At the surface of the nanoparticle array, strongly localized electromagnetic fields in engineered plasmonic/polaritonic structures allow for single base resolution using optical techniques.

Inventors:
; ; ; ; ;
Issue Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Univ. of New Mexico, Albuquerque, NM (United States)
Sponsoring Org.:
USDOE; US Army Research Office (ARO)
OSTI Identifier:
1823813
Patent Number(s):
10969364
Application Number:
15/896,843
Assignee:
STC.UNM (Albuquerque, NM)
Patent Classifications (CPCs):
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
AC04-94AL85000; FG02-02ER15368; W911NF-08-1-0214; W911NF-11-00080
Resource Type:
Patent
Resource Relation:
Patent File Date: 02/14/2018
Country of Publication:
United States
Language:
English

Citation Formats

Brueck, Steven R. J., Edwards, Jeremy Scott, Neumann, Alexander, Kuznetsova, Yuliya, Mendoza, Edgar A., and Brinker, C. Jeffrey. Innovative nanopore sequencing technology including a self-assembled porous membrane. United States: N. p., 2021. Web.
Brueck, Steven R. J., Edwards, Jeremy Scott, Neumann, Alexander, Kuznetsova, Yuliya, Mendoza, Edgar A., & Brinker, C. Jeffrey. Innovative nanopore sequencing technology including a self-assembled porous membrane. United States.
Brueck, Steven R. J., Edwards, Jeremy Scott, Neumann, Alexander, Kuznetsova, Yuliya, Mendoza, Edgar A., and Brinker, C. Jeffrey. Tue . "Innovative nanopore sequencing technology including a self-assembled porous membrane". United States. https://www.osti.gov/servlets/purl/1823813.
@article{osti_1823813,
title = {Innovative nanopore sequencing technology including a self-assembled porous membrane},
author = {Brueck, Steven R. J. and Edwards, Jeremy Scott and Neumann, Alexander and Kuznetsova, Yuliya and Mendoza, Edgar A. and Brinker, C. Jeffrey},
abstractNote = {Methods and apparatus for long read, label-free, optical nanopore long chain molecule sequencing. In general, the present disclosure describes a novel sequencing technology based on the integration of nanochannels to deliver single long-chain molecules with widely spaced (>wavelength), ˜1-nm aperture “tortuous” nanopores that slow translocation sufficiently to provide massively parallel, single base resolution using optical techniques. A novel, directed self-assembly nanofabrication scheme using simple colloidal nanoparticles is used to form the nanopore arrays atop nanochannels that unfold the long chain molecules. At the surface of the nanoparticle array, strongly localized electromagnetic fields in engineered plasmonic/polaritonic structures allow for single base resolution using optical techniques.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2021},
month = {4}
}

Works referenced in this record:

Method for detecting a resonant nonlinear optical signal and device for implementing said method
patent-application, January 2013


Methods for performing reflow in bonding processes
patent, May 2013


Artificial receptors
patent-application, March 2008


Combinatorial multidomain mesoporous chips and a method for fractionation, stabilization, and storage of biomolecules
patent-application, March 2011


Nanochannel Arrays and Their Preparation and Use for High Throughput Macromolecular Analysis
patent-application, May 2015