Innovative nanopore sequencing technology including a self-assembled porous membrane
Abstract
Methods and apparatus for long read, label-free, optical nanopore long chain molecule sequencing. In general, the present disclosure describes a novel sequencing technology based on the integration of nanochannels to deliver single long-chain molecules with widely spaced (>wavelength), ˜1-nm aperture “tortuous” nanopores that slow translocation sufficiently to provide massively parallel, single base resolution using optical techniques. A novel, directed self-assembly nanofabrication scheme using simple colloidal nanoparticles is used to form the nanopore arrays atop nanochannels that unfold the long chain molecules. At the surface of the nanoparticle array, strongly localized electromagnetic fields in engineered plasmonic/polaritonic structures allow for single base resolution using optical techniques.
- Inventors:
- Issue Date:
- Research Org.:
- Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Univ. of New Mexico, Albuquerque, NM (United States)
- Sponsoring Org.:
- USDOE; US Army Research Office (ARO)
- OSTI Identifier:
- 1823813
- Patent Number(s):
- 10969364
- Application Number:
- 15/896,843
- Assignee:
- STC.UNM (Albuquerque, NM)
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- AC04-94AL85000; FG02-02ER15368; W911NF-08-1-0214; W911NF-11-00080
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 02/14/2018
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Brueck, Steven R. J., Edwards, Jeremy Scott, Neumann, Alexander, Kuznetsova, Yuliya, Mendoza, Edgar A., and Brinker, C. Jeffrey. Innovative nanopore sequencing technology including a self-assembled porous membrane. United States: N. p., 2021.
Web.
Brueck, Steven R. J., Edwards, Jeremy Scott, Neumann, Alexander, Kuznetsova, Yuliya, Mendoza, Edgar A., & Brinker, C. Jeffrey. Innovative nanopore sequencing technology including a self-assembled porous membrane. United States.
Brueck, Steven R. J., Edwards, Jeremy Scott, Neumann, Alexander, Kuznetsova, Yuliya, Mendoza, Edgar A., and Brinker, C. Jeffrey. Tue .
"Innovative nanopore sequencing technology including a self-assembled porous membrane". United States. https://www.osti.gov/servlets/purl/1823813.
@article{osti_1823813,
title = {Innovative nanopore sequencing technology including a self-assembled porous membrane},
author = {Brueck, Steven R. J. and Edwards, Jeremy Scott and Neumann, Alexander and Kuznetsova, Yuliya and Mendoza, Edgar A. and Brinker, C. Jeffrey},
abstractNote = {Methods and apparatus for long read, label-free, optical nanopore long chain molecule sequencing. In general, the present disclosure describes a novel sequencing technology based on the integration of nanochannels to deliver single long-chain molecules with widely spaced (>wavelength), ˜1-nm aperture “tortuous” nanopores that slow translocation sufficiently to provide massively parallel, single base resolution using optical techniques. A novel, directed self-assembly nanofabrication scheme using simple colloidal nanoparticles is used to form the nanopore arrays atop nanochannels that unfold the long chain molecules. At the surface of the nanoparticle array, strongly localized electromagnetic fields in engineered plasmonic/polaritonic structures allow for single base resolution using optical techniques.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2021},
month = {4}
}
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