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Title: Production of organic acids from Aspergillus cis-aconitic acid decarboxylase (cadA) deletion strains

Abstract

This application provides recombinant Aspergillus fungi having an endogenous cis-aconitic acid decarboxylase (cadA) gene genetically inactivated, which allows aconitic acid production by the recombinant fungi. Such recombinant fungi can further include an exogenous nucleic acid molecule encoding aspartate decarboxylase (panD), an exogenous nucleic acid molecule encoding β-alanine-pyruvate aminotransferase (BAPAT), and an exogenous nucleic acid molecule encoding 3-hydroxypropionate dehydrogenase (HPDH). Kits including these fungi, and methods of using these fungi to produce aconitic acid and 3-hydroxypropionic acid (3-HP) are also provided.

Inventors:
; ;
Issue Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1805627
Patent Number(s):
10947548
Application Number:
16/393,149
Assignee:
Battelle Memorial Institute (Richland, WA)
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Patent
Resource Relation:
Patent File Date: 04/24/2019
Country of Publication:
United States
Language:
English

Citation Formats

Deng, Shuang, Dai, Ziyu, and Magnuson, Jon K. Production of organic acids from Aspergillus cis-aconitic acid decarboxylase (cadA) deletion strains. United States: N. p., 2021. Web.
Deng, Shuang, Dai, Ziyu, & Magnuson, Jon K. Production of organic acids from Aspergillus cis-aconitic acid decarboxylase (cadA) deletion strains. United States.
Deng, Shuang, Dai, Ziyu, and Magnuson, Jon K. Tue . "Production of organic acids from Aspergillus cis-aconitic acid decarboxylase (cadA) deletion strains". United States. https://www.osti.gov/servlets/purl/1805627.
@article{osti_1805627,
title = {Production of organic acids from Aspergillus cis-aconitic acid decarboxylase (cadA) deletion strains},
author = {Deng, Shuang and Dai, Ziyu and Magnuson, Jon K.},
abstractNote = {This application provides recombinant Aspergillus fungi having an endogenous cis-aconitic acid decarboxylase (cadA) gene genetically inactivated, which allows aconitic acid production by the recombinant fungi. Such recombinant fungi can further include an exogenous nucleic acid molecule encoding aspartate decarboxylase (panD), an exogenous nucleic acid molecule encoding β-alanine-pyruvate aminotransferase (BAPAT), and an exogenous nucleic acid molecule encoding 3-hydroxypropionate dehydrogenase (HPDH). Kits including these fungi, and methods of using these fungi to produce aconitic acid and 3-hydroxypropionic acid (3-HP) are also provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2021},
month = {3}
}