In vitro assembly of bacterial microcompartments
Abstract
The present disclosure is related to a BMC fusion protein that is capable of in vitro assembly, comprising a constituent BMC shell protein subunit and a sterically hindering protein domain that is cleavable. The BMC fusion protein is capable of in vitro assembly triggered by removal of the fused sterically hindering domain. The present disclosure is also related to a means to produce BMC shells in vitro, triggered by removal of a fused sterically hindering domain from one or more constituent BMC shell protein subunits. The BMC fusion protein enables encapsulation of broad classes of materials and biophysical studies of shell assembly, encapsulation, and permeability that would otherwise be unavailable from BMCs assembled in vivo.
- Inventors:
- Issue Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1805489
- Patent Number(s):
- 10913777
- Application Number:
- 15/985,218
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- AC02-05CH11231; FG02-91ER20021
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 05/21/2018
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Hagen, Andrew Ronald, Kerfeld, Cheryl A., and Sutter, Markus. In vitro assembly of bacterial microcompartments. United States: N. p., 2021.
Web.
Hagen, Andrew Ronald, Kerfeld, Cheryl A., & Sutter, Markus. In vitro assembly of bacterial microcompartments. United States.
Hagen, Andrew Ronald, Kerfeld, Cheryl A., and Sutter, Markus. Tue .
"In vitro assembly of bacterial microcompartments". United States. https://www.osti.gov/servlets/purl/1805489.
@article{osti_1805489,
title = {In vitro assembly of bacterial microcompartments},
author = {Hagen, Andrew Ronald and Kerfeld, Cheryl A. and Sutter, Markus},
abstractNote = {The present disclosure is related to a BMC fusion protein that is capable of in vitro assembly, comprising a constituent BMC shell protein subunit and a sterically hindering protein domain that is cleavable. The BMC fusion protein is capable of in vitro assembly triggered by removal of the fused sterically hindering domain. The present disclosure is also related to a means to produce BMC shells in vitro, triggered by removal of a fused sterically hindering domain from one or more constituent BMC shell protein subunits. The BMC fusion protein enables encapsulation of broad classes of materials and biophysical studies of shell assembly, encapsulation, and permeability that would otherwise be unavailable from BMCs assembled in vivo.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2021},
month = {2}
}
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Works referenced in this record:
Methods for Producing Microcompartments
patent-application, April 2017
- Kerfeld, Cheryl A.; Lassila, Jonathan K.; Kinney, James N.
- US Patent Application 15/367089; 20170107523
Design and Implementation of Novel and/or Enhanced Bacterial Microcompartments for Customizing Metabolis
patent-application, August 2012
- Kerfeld, Cheryl A.; Laque, Dominique
- US Patent Application 13/367260; 20120210459
Production and purification of a protein fused to a binding protein
patent, July 1997
- Guan, Chudi; Inouye, Hiroshi; Chang, Frank N.
- US Patent Document 5,643,758
Rapidly cleavable sumo fusion protein expression system for difficult to express proteins
patent, March 2005
- Lima, Christopher D.; Mossessova, Elena
- US Patent Document 6,872,551
Constructs and Systems and Methods for Producing Microcompartments
patent-application, January 2015
- Kerfeld, Cheryl A.; Lassila, Jonathan K.; Kinney, James N.
- US Patent Application 14/214172; 20150026840