Methods of making polypeptides with non-standard amino acids using genomically recoded organisms
Abstract
A method of making a polypeptide including at least one covalent bond between a pair of reactive side chains of corresponding amino acids, wherein the covalent bond is insensitive to reduction is provided including genetically modifying a genomically recoded organism to express a corresponding synthetase, tRNA or synthetase/tRNA pair for translating mRNA encoding the corresponding amino acids having the reactive side chains into the polypeptide and to express the polypeptide including the at least one pair of the reactive side chains wherein the reactive side chains are oriented near one another when the expressed polypeptide is in a folded configuration, wherein the reactive side chains react to form the covalent bond that is insensitive to reduction.
- Inventors:
- Issue Date:
- Research Org.:
- Harvard Univ., Cambridge, MA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1805474
- Patent Number(s):
- 10910085
- Application Number:
- 15/522,569
- Assignee:
- President and Fellows of Harvard College (Cambridge, MA)
- DOE Contract Number:
- FG02-02ER63445
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 10/28/2015
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Church, George M., Gregg, Christopher J., Lajoie, Marc J., and Mandell, Daniel J. Methods of making polypeptides with non-standard amino acids using genomically recoded organisms. United States: N. p., 2021.
Web.
Church, George M., Gregg, Christopher J., Lajoie, Marc J., & Mandell, Daniel J. Methods of making polypeptides with non-standard amino acids using genomically recoded organisms. United States.
Church, George M., Gregg, Christopher J., Lajoie, Marc J., and Mandell, Daniel J. Tue .
"Methods of making polypeptides with non-standard amino acids using genomically recoded organisms". United States. https://www.osti.gov/servlets/purl/1805474.
@article{osti_1805474,
title = {Methods of making polypeptides with non-standard amino acids using genomically recoded organisms},
author = {Church, George M. and Gregg, Christopher J. and Lajoie, Marc J. and Mandell, Daniel J.},
abstractNote = {A method of making a polypeptide including at least one covalent bond between a pair of reactive side chains of corresponding amino acids, wherein the covalent bond is insensitive to reduction is provided including genetically modifying a genomically recoded organism to express a corresponding synthetase, tRNA or synthetase/tRNA pair for translating mRNA encoding the corresponding amino acids having the reactive side chains into the polypeptide and to express the polypeptide including the at least one pair of the reactive side chains wherein the reactive side chains are oriented near one another when the expressed polypeptide is in a folded configuration, wherein the reactive side chains react to form the covalent bond that is insensitive to reduction.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2021},
month = {2}
}