Compositions and methods for making selenocysteine containing polypeptides

Soll, Dieter; Aldag, Caroline; Hohn, Michael; Mukai, Takahito

Title: Compositions and methods for making selenocysteine containing polypeptides

Abstract

Non-naturally occurring tRNASec and methods of using them for recombinant expression of proteins engineered to include one or more selenocysteine residues are disclosed. The non-naturally occurring tRNASec can be used for recombinant manufacture of selenocysteine containing polypeptides encoded by mRNA without the requirement of an SECIS element. In some embodiments, selenocysteine containing polypeptides are manufactured by co-expressing a non-naturally occurring tRNASec a recombinant expression system, such as E. coli, with SerRS, EF-Tu, SelA, or PSTK and SepSecS, and an mRNA with at least one codon that recognizes the anticodon of the non-naturally occurring tRNASec.

Inventors:: Soll, Dieter; Aldag, Caroline; Hohn, Michael; Mukai, Takahito

Issue Date:: 2020-12-29

Research Org.:: Yale Univ., New Haven, CT (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1771764

Patent Number(s):: 10876142

Application Number:: 15/724,678

Assignee:: Yale University (New Haven, CT)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P21/00 - Preparation of peptides or proteins

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/11 - DNA or RNA fragments

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DOE Contract Number:: FG02-98ER20311

Resource Type:: Patent

Resource Relation:: Patent File Date: 10/04/2017

Country of Publication:: United States

Language:: English

Citation Formats


                    Soll, Dieter, Aldag, Caroline, Hohn, Michael, and Mukai, Takahito. Compositions and methods for making selenocysteine containing polypeptides.  United States: N. p., 2020. 
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                    Soll, Dieter, Aldag, Caroline, Hohn, Michael, & Mukai, Takahito. Compositions and methods for making selenocysteine containing polypeptides.  United States.

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                    Soll, Dieter, Aldag, Caroline, Hohn, Michael, and Mukai, Takahito. Tue .  
        "Compositions and methods for making selenocysteine containing polypeptides".  United States.  https://www.osti.gov/servlets/purl/1771764.

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@article{osti_1771764,

  title        = {Compositions and methods for making selenocysteine containing polypeptides},

  author       = {Soll, Dieter and Aldag, Caroline and Hohn, Michael and Mukai, Takahito},

  abstractNote = {Non-naturally occurring tRNASec and methods of using them for recombinant expression of proteins engineered to include one or more selenocysteine residues are disclosed. The non-naturally occurring tRNASec can be used for recombinant manufacture of selenocysteine containing polypeptides encoded by mRNA without the requirement of an SECIS element. In some embodiments, selenocysteine containing polypeptides are manufactured by co-expressing a non-naturally occurring tRNASec a recombinant expression system, such as E. coli, with SerRS, EF-Tu, SelA, or PSTK and SepSecS, and an mRNA with at least one codon that recognizes the anticodon of the non-naturally occurring tRNASec.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2020},

  month        = {12}

}

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