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Title: Compositions and methods for making selenocysteine containing polypeptides


Non-naturally occurring tRNASec and methods of using them for recombinant expression of proteins engineered to include one or more selenocysteine residues are disclosed. The non-naturally occurring tRNASec can be used for recombinant manufacture of selenocysteine containing polypeptides encoded by mRNA without the requirement of an SECIS element. In some embodiments, selenocysteine containing polypeptides are manufactured by co-expressing a non-naturally occurring tRNASec a recombinant expression system, such as E. coli, with SerRS, EF-Tu, SelA, or PSTK and SepSecS, and an mRNA with at least one codon that recognizes the anticodon of the non-naturally occurring tRNASec.

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Research Org.:
Yale Univ., New Haven, CT (United States)
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Yale University (New Haven, CT)
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Patent File Date: 10/04/2017
Country of Publication:
United States

Citation Formats

Soll, Dieter, Aldag, Caroline, Hohn, Michael, and Mukai, Takahito. Compositions and methods for making selenocysteine containing polypeptides. United States: N. p., 2020. Web.
Soll, Dieter, Aldag, Caroline, Hohn, Michael, & Mukai, Takahito. Compositions and methods for making selenocysteine containing polypeptides. United States.
Soll, Dieter, Aldag, Caroline, Hohn, Michael, and Mukai, Takahito. Tue . "Compositions and methods for making selenocysteine containing polypeptides". United States.
title = {Compositions and methods for making selenocysteine containing polypeptides},
author = {Soll, Dieter and Aldag, Caroline and Hohn, Michael and Mukai, Takahito},
abstractNote = {Non-naturally occurring tRNASec and methods of using them for recombinant expression of proteins engineered to include one or more selenocysteine residues are disclosed. The non-naturally occurring tRNASec can be used for recombinant manufacture of selenocysteine containing polypeptides encoded by mRNA without the requirement of an SECIS element. In some embodiments, selenocysteine containing polypeptides are manufactured by co-expressing a non-naturally occurring tRNASec a recombinant expression system, such as E. coli, with SerRS, EF-Tu, SelA, or PSTK and SepSecS, and an mRNA with at least one codon that recognizes the anticodon of the non-naturally occurring tRNASec.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2020},
month = {12}

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