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Title: Barcoded protein array for multiplex single-molecule interaction profiling

Abstract

Methods for attaching barcodes to polypeptides are provided. Methods for detecting molecular interactions at the single molecule level are provided. Embodiments of the invention are directed to a ONA barcoded protein array technology for parallel protein interaction profiling on a single molecule basis. DNA barcodes are attached to proteins collectively via ribosome display or individually via enzymatic conjugation. Novel methods are described herein that measure protein interactions based on the statistical analysis of co-localized polonies arising from barcoding DNAs of interacting proteins.

Inventors:
;
Issue Date:
Research Org.:
Harvard Univ., Cambridge, MA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1771708
Patent Number(s):
10858698
Application Number:
15/128,145
Assignee:
President and Fellows of Harvard College (Cambridge, MA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
FG02-02ER63445
Resource Type:
Patent
Resource Relation:
Patent File Date: 03/25/2015
Country of Publication:
United States
Language:
English

Citation Formats

Church, George M., and Gu, Liangcai. Barcoded protein array for multiplex single-molecule interaction profiling. United States: N. p., 2020. Web.
Church, George M., & Gu, Liangcai. Barcoded protein array for multiplex single-molecule interaction profiling. United States.
Church, George M., and Gu, Liangcai. Tue . "Barcoded protein array for multiplex single-molecule interaction profiling". United States. https://www.osti.gov/servlets/purl/1771708.
@article{osti_1771708,
title = {Barcoded protein array for multiplex single-molecule interaction profiling},
author = {Church, George M. and Gu, Liangcai},
abstractNote = {Methods for attaching barcodes to polypeptides are provided. Methods for detecting molecular interactions at the single molecule level are provided. Embodiments of the invention are directed to a ONA barcoded protein array technology for parallel protein interaction profiling on a single molecule basis. DNA barcodes are attached to proteins collectively via ribosome display or individually via enzymatic conjugation. Novel methods are described herein that measure protein interactions based on the statistical analysis of co-localized polonies arising from barcoding DNAs of interacting proteins.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2020},
month = {12}
}

Works referenced in this record:

Nuclear Factor I genomic binding associates with chromatin boundaries
journal, January 2013


A Universal DNA-Based Protein Detection System
journal, September 2013


Multiplex single-molecule interaction profiling of DNA-barcoded proteins
journal, September 2014