Frequency-based modulation of diverse species in a nucleic acid library
Abstract
A method of processing a collection of nucleic acid sequences is provided including connecting an adaptor to one or more or each nucleic acid sequence in the collection to create a processed nucleic acid template library, wherein the adaptor includes a first DNA sequence encoding a PAM sequence and at least a tracr mate.
- Inventors:
- Issue Date:
- Research Org.:
- Harvard Univ., Cambridge, MA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1771684
- Patent Number(s):
- 10851369
- Application Number:
- 16/310,643
- Assignee:
- President and Fellows of Harvard College (Cambridge, MA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- FG02-02ER63445
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 06/21/2017
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Kalhor, Reza, Juarez, Javier Fernandez, Lee, Henry Hung-yi, and Church, George M. Frequency-based modulation of diverse species in a nucleic acid library. United States: N. p., 2020.
Web.
Kalhor, Reza, Juarez, Javier Fernandez, Lee, Henry Hung-yi, & Church, George M. Frequency-based modulation of diverse species in a nucleic acid library. United States.
Kalhor, Reza, Juarez, Javier Fernandez, Lee, Henry Hung-yi, and Church, George M. Tue .
"Frequency-based modulation of diverse species in a nucleic acid library". United States. https://www.osti.gov/servlets/purl/1771684.
@article{osti_1771684,
title = {Frequency-based modulation of diverse species in a nucleic acid library},
author = {Kalhor, Reza and Juarez, Javier Fernandez and Lee, Henry Hung-yi and Church, George M.},
abstractNote = {A method of processing a collection of nucleic acid sequences is provided including connecting an adaptor to one or more or each nucleic acid sequence in the collection to create a processed nucleic acid template library, wherein the adaptor includes a first DNA sequence encoding a PAM sequence and at least a tracr mate.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Dec 01 00:00:00 EST 2020},
month = {Tue Dec 01 00:00:00 EST 2020}
}
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