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Title: Frequency-based modulation of diverse species in a nucleic acid library

Abstract

A method of processing a collection of nucleic acid sequences is provided including connecting an adaptor to one or more or each nucleic acid sequence in the collection to create a processed nucleic acid template library, wherein the adaptor includes a first DNA sequence encoding a PAM sequence and at least a tracr mate.

Inventors:
; ; ;
Issue Date:
Research Org.:
Harvard Univ., Cambridge, MA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1771684
Patent Number(s):
10851369
Application Number:
16/310,643
Assignee:
President and Fellows of Harvard College (Cambridge, MA)
Patent Classifications (CPCs):
C - CHEMISTRY C40 - COMBINATORIAL TECHNOLOGY C40B - COMBINATORIAL CHEMISTRY
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FG02-02ER63445
Resource Type:
Patent
Resource Relation:
Patent File Date: 06/21/2017
Country of Publication:
United States
Language:
English

Citation Formats

Kalhor, Reza, Juarez, Javier Fernandez, Lee, Henry Hung-yi, and Church, George M. Frequency-based modulation of diverse species in a nucleic acid library. United States: N. p., 2020. Web.
Kalhor, Reza, Juarez, Javier Fernandez, Lee, Henry Hung-yi, & Church, George M. Frequency-based modulation of diverse species in a nucleic acid library. United States.
Kalhor, Reza, Juarez, Javier Fernandez, Lee, Henry Hung-yi, and Church, George M. Tue . "Frequency-based modulation of diverse species in a nucleic acid library". United States. https://www.osti.gov/servlets/purl/1771684.
@article{osti_1771684,
title = {Frequency-based modulation of diverse species in a nucleic acid library},
author = {Kalhor, Reza and Juarez, Javier Fernandez and Lee, Henry Hung-yi and Church, George M.},
abstractNote = {A method of processing a collection of nucleic acid sequences is provided including connecting an adaptor to one or more or each nucleic acid sequence in the collection to create a processed nucleic acid template library, wherein the adaptor includes a first DNA sequence encoding a PAM sequence and at least a tracr mate.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2020},
month = {12}
}

Works referenced in this record:

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System
journal, October 2015


Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements
journal, November 2015


A scalable, fully automated process for construction of sequence-ready barcoded libraries for 454
journal, January 2010