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Title: In vitro methods for processing lignin and other aromatic compounds

Abstract

Enzymes for depolymerizing lignin. The enzymes include dehydrogenases, β-etherases, and glutathione lyases. The dehydrogenases can comprise one or more or LigD, LigO, LigN, and LigL. The β-etherases can comprise one or more of LigE, LigF, LigP, and BaeA. The glutathione lyases can comprise any one or more of LigG and a number of non-stereospecific, optionally recombinant glutathione lyases derived from Sphingobium sp. SYK-6, Novosphingobium aromaticivorans, Escherichia coli, Streptococcus sanguinis, Phanerochaete chrysosporium, and other microorganisms. The enzymes can be combined in compositions and/or used in methods of processing lignin or other aromatic compounds in vitro.

Inventors:
; ; ; ; ; ; ;
Issue Date:
Research Org.:
Univ. of Wisconsin, Madison, WI (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1771603
Patent Number(s):
10829745
Application Number:
16/103,275
Assignee:
Wisconsin Alumni Research Foundation (Madison, WI)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02P - CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
DOE Contract Number:  
FC02-07ER64494; SC0018409
Resource Type:
Patent
Resource Relation:
Patent File Date: 08/14/2018
Country of Publication:
United States
Language:
English

Citation Formats

Donohue, Timothy James, Gall, Daniel Leo, Kontur, Wayne S., Kim, Hoon Seok, Ralph, John, Noguera, Daniel R., Fox, Brian G., and Bingman, Craig. In vitro methods for processing lignin and other aromatic compounds. United States: N. p., 2020. Web.
Donohue, Timothy James, Gall, Daniel Leo, Kontur, Wayne S., Kim, Hoon Seok, Ralph, John, Noguera, Daniel R., Fox, Brian G., & Bingman, Craig. In vitro methods for processing lignin and other aromatic compounds. United States.
Donohue, Timothy James, Gall, Daniel Leo, Kontur, Wayne S., Kim, Hoon Seok, Ralph, John, Noguera, Daniel R., Fox, Brian G., and Bingman, Craig. Tue . "In vitro methods for processing lignin and other aromatic compounds". United States. https://www.osti.gov/servlets/purl/1771603.
@article{osti_1771603,
title = {In vitro methods for processing lignin and other aromatic compounds},
author = {Donohue, Timothy James and Gall, Daniel Leo and Kontur, Wayne S. and Kim, Hoon Seok and Ralph, John and Noguera, Daniel R. and Fox, Brian G. and Bingman, Craig},
abstractNote = {Enzymes for depolymerizing lignin. The enzymes include dehydrogenases, β-etherases, and glutathione lyases. The dehydrogenases can comprise one or more or LigD, LigO, LigN, and LigL. The β-etherases can comprise one or more of LigE, LigF, LigP, and BaeA. The glutathione lyases can comprise any one or more of LigG and a number of non-stereospecific, optionally recombinant glutathione lyases derived from Sphingobium sp. SYK-6, Novosphingobium aromaticivorans, Escherichia coli, Streptococcus sanguinis, Phanerochaete chrysosporium, and other microorganisms. The enzymes can be combined in compositions and/or used in methods of processing lignin or other aromatic compounds in vitro.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2020},
month = {11}
}

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