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Title: Multiplexed binary assembly and quantitative tracking of bacterial populations

Abstract

Compositions and methods for tracking combinations of mutations in populations by coupling DNA assembly and paired-end sequencing technology with high-throughput sequencing. This allows for an unlimited number of mutation sites in a population of microorganisms to be identified, and allows sites to be sequenced across several replicates. Unique identifiers (DNA barcodes) can be used to measure fitness data by sequencing of the barcodes multiplexed with the mutation sites with high-throughput short sequencing read technology, allowing selections to be rapidly performed on populations of known combinatorial genotypes.

Inventors:
;
Issue Date:
Research Org.:
Univ. of Colorado, Denver, CO (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1771467
Patent Number(s):
10793851
Application Number:
15/749,540
Assignee:
The Regents of the University of Colorado, a body corporate (Denver, CO)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C40 - COMBINATORIAL TECHNOLOGY C40B - COMBINATORIAL CHEMISTRY
DOE Contract Number:  
SC0008812
Resource Type:
Patent
Resource Relation:
Patent File Date: 08/04/2016
Country of Publication:
United States
Language:
English

Citation Formats

Gill, Ryan T., and Zeitoun, Ramsey Ibrahim. Multiplexed binary assembly and quantitative tracking of bacterial populations. United States: N. p., 2020. Web.
Gill, Ryan T., & Zeitoun, Ramsey Ibrahim. Multiplexed binary assembly and quantitative tracking of bacterial populations. United States.
Gill, Ryan T., and Zeitoun, Ramsey Ibrahim. Tue . "Multiplexed binary assembly and quantitative tracking of bacterial populations". United States. https://www.osti.gov/servlets/purl/1771467.
@article{osti_1771467,
title = {Multiplexed binary assembly and quantitative tracking of bacterial populations},
author = {Gill, Ryan T. and Zeitoun, Ramsey Ibrahim},
abstractNote = {Compositions and methods for tracking combinations of mutations in populations by coupling DNA assembly and paired-end sequencing technology with high-throughput sequencing. This allows for an unlimited number of mutation sites in a population of microorganisms to be identified, and allows sites to be sequenced across several replicates. Unique identifiers (DNA barcodes) can be used to measure fitness data by sequencing of the barcodes multiplexed with the mutation sites with high-throughput short sequencing read technology, allowing selections to be rapidly performed on populations of known combinatorial genotypes.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2020},
month = {10}
}

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