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Title: Miniaturized, automated in-vitro tissue bioreactor

Abstract

In one embodiment, a system includes a bioreactor coupled to a substrate. The bioreactor includes: a plurality of walls defining a reservoir; a plurality of fluidic channels in at least some of the walls; a fluidic inlet in fluidic communication with the reservoir via, the fluidic channels; a fluidic outlet in fluidic communication with the reservoir via the fluidic channels; and one or more sensors coupled to the reservoir, each sensor being configured to detect one or more of: environmental conditions in the reservoir; and physiological conditions of one or more cells optionally present in the reservoir. In another embodiment, a method includes delivering media to a reservoir of a bioreactor; delivering a plurality of cells to the reservoir, controlling a reservoir temperature and a reservoir gas composition; using one or more of the sensors to monitor environmental and physiological conditions; and reporting the environmental and/or physiological conditions.

Inventors:
; ; ; ; ; ; ; ; ;
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1771466
Patent Number(s):
10793820
Application Number:
14/265,019
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12M - APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
DOE Contract Number:  
AC52-07NA27344
Resource Type:
Patent
Resource Relation:
Patent File Date: 04/29/2014
Country of Publication:
United States
Language:
English

Citation Formats

Sheth, Heeral, Chen, Haiyin, Hudson, Bryan D., Kulp, Kris, Mcnerney, Margaret W., Pannu, Satinderpall S., Qian, Fang, Tooker, Angela C., Wheeler, Elizabeth K., and Tolosa, Vanessa. Miniaturized, automated in-vitro tissue bioreactor. United States: N. p., 2020. Web.
Sheth, Heeral, Chen, Haiyin, Hudson, Bryan D., Kulp, Kris, Mcnerney, Margaret W., Pannu, Satinderpall S., Qian, Fang, Tooker, Angela C., Wheeler, Elizabeth K., & Tolosa, Vanessa. Miniaturized, automated in-vitro tissue bioreactor. United States.
Sheth, Heeral, Chen, Haiyin, Hudson, Bryan D., Kulp, Kris, Mcnerney, Margaret W., Pannu, Satinderpall S., Qian, Fang, Tooker, Angela C., Wheeler, Elizabeth K., and Tolosa, Vanessa. Tue . "Miniaturized, automated in-vitro tissue bioreactor". United States. https://www.osti.gov/servlets/purl/1771466.
@article{osti_1771466,
title = {Miniaturized, automated in-vitro tissue bioreactor},
author = {Sheth, Heeral and Chen, Haiyin and Hudson, Bryan D. and Kulp, Kris and Mcnerney, Margaret W. and Pannu, Satinderpall S. and Qian, Fang and Tooker, Angela C. and Wheeler, Elizabeth K. and Tolosa, Vanessa},
abstractNote = {In one embodiment, a system includes a bioreactor coupled to a substrate. The bioreactor includes: a plurality of walls defining a reservoir; a plurality of fluidic channels in at least some of the walls; a fluidic inlet in fluidic communication with the reservoir via, the fluidic channels; a fluidic outlet in fluidic communication with the reservoir via the fluidic channels; and one or more sensors coupled to the reservoir, each sensor being configured to detect one or more of: environmental conditions in the reservoir; and physiological conditions of one or more cells optionally present in the reservoir. In another embodiment, a method includes delivering media to a reservoir of a bioreactor; delivering a plurality of cells to the reservoir, controlling a reservoir temperature and a reservoir gas composition; using one or more of the sensors to monitor environmental and physiological conditions; and reporting the environmental and/or physiological conditions.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2020},
month = {10}
}

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