Title: Miniaturized, automated in-vitro tissue bioreactor

Abstract

In one embodiment, a system includes a bioreactor coupled to a substrate. The bioreactor includes: a plurality of walls defining a reservoir; a plurality of fluidic channels in at least some of the walls; a fluidic inlet in fluidic communication with the reservoir via, the fluidic channels; a fluidic outlet in fluidic communication with the reservoir via the fluidic channels; and one or more sensors coupled to the reservoir, each sensor being configured to detect one or more of: environmental conditions in the reservoir; and physiological conditions of one or more cells optionally present in the reservoir. In another embodiment, a method includes delivering media to a reservoir of a bioreactor; delivering a plurality of cells to the reservoir, controlling a reservoir temperature and a reservoir gas composition; using one or more of the sensors to monitor environmental and physiological conditions; and reporting the environmental and/or physiological conditions.

Inventors:

Sheth, Heeral; Chen, Haiyin; Hudson, Bryan D.; Kulp, Kris; Mcnerney, Margaret W.; Pannu, Satinderpall S.; Qian, Fang; Tooker, Angela C.; Wheeler, Elizabeth K.; Tolosa, Vanessa

Issue Date:

2020-10-06

Research Org.:

Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1771466

Patent Number(s):

10793820

Application Number:

14/265,019

Assignee:

Lawrence Livermore National Security, LLC (Livermore, CA)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12M - APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY

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C - CHEMISTRY C12 - BIOCHEMISTRY C12M - APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY

C12M35/02 - {Electrical or electromagnetic means, e.g. for electroporation or for cell fusion}
C12M41/48 - {Automatic or computerized control
C12M41/46 - {of cellular or enzymatic activity or functionality, e.g. cell viability}
C12M41/26 - {of pH}

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DOE Contract Number:  

AC52-07NA27344

Resource Type:

Patent

Resource Relation:

Patent File Date: 04/29/2014

Country of Publication:

United States

Language:

English