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Title: Engineering an increase in ethanol production by altering cofactor specificity

Abstract

The present invention provides for the manipulation of cofactor usage in a recombinant host cell to increase the formation of desirable products. In some embodiments, the invention provides for a recombinant microorganism comprising a mutation in one or more native enzymes such that their cofactor specificity is altered in such a way that overall cofactor usage in the cell is balanced for a specified pathway and there is an increase in a specific product formation within the cell. In some embodiments, endogenous enzymes are replaced by enzymes with an alternate cofactor specificity from a different species.

Inventors:
; ; ; ; ; ; ;
Issue Date:
Research Org.:
Enchi Corp., Wellesley Hills, MA (United States); Dartmouth College, Hanover, NH (United States); UT-Battelle LLC/ORNL, Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1735231
Patent Number(s):
10767196
Application Number:
14/361,948
Assignee:
Enchi Corporation (Wellesley Hills, MA); Trustees of Dartmouth College (Hanover, NH); UT-Battelle, LLC (Oak Ridge, TN)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
POS2-06ER64304; FC36-07GO17057
Resource Type:
Patent
Resource Relation:
Patent File Date: 11/30/2012
Country of Publication:
United States
Language:
English

Citation Formats

Lo, Jonathan, Guss, Adam M., van Dijken, Johannes Pieter, Shaw, IV, Arthur J., Olson, Daniel G., Herring, Christopher D., Argyros, D. Aaron, and Caiazza, Nicky. Engineering an increase in ethanol production by altering cofactor specificity. United States: N. p., 2020. Web.
Lo, Jonathan, Guss, Adam M., van Dijken, Johannes Pieter, Shaw, IV, Arthur J., Olson, Daniel G., Herring, Christopher D., Argyros, D. Aaron, & Caiazza, Nicky. Engineering an increase in ethanol production by altering cofactor specificity. United States.
Lo, Jonathan, Guss, Adam M., van Dijken, Johannes Pieter, Shaw, IV, Arthur J., Olson, Daniel G., Herring, Christopher D., Argyros, D. Aaron, and Caiazza, Nicky. Tue . "Engineering an increase in ethanol production by altering cofactor specificity". United States. https://www.osti.gov/servlets/purl/1735231.
@article{osti_1735231,
title = {Engineering an increase in ethanol production by altering cofactor specificity},
author = {Lo, Jonathan and Guss, Adam M. and van Dijken, Johannes Pieter and Shaw, IV, Arthur J. and Olson, Daniel G. and Herring, Christopher D. and Argyros, D. Aaron and Caiazza, Nicky},
abstractNote = {The present invention provides for the manipulation of cofactor usage in a recombinant host cell to increase the formation of desirable products. In some embodiments, the invention provides for a recombinant microorganism comprising a mutation in one or more native enzymes such that their cofactor specificity is altered in such a way that overall cofactor usage in the cell is balanced for a specified pathway and there is an increase in a specific product formation within the cell. In some embodiments, endogenous enzymes are replaced by enzymes with an alternate cofactor specificity from a different species.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2020},
month = {9}
}

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