Title: Engineering an increase in ethanol production by altering cofactor specificity

Abstract

The present invention provides for the manipulation of cofactor usage in a recombinant host cell to increase the formation of desirable products. In some embodiments, the invention provides for a recombinant microorganism comprising a mutation in one or more native enzymes such that their cofactor specificity is altered in such a way that overall cofactor usage in the cell is balanced for a specified pathway and there is an increase in a specific product formation within the cell. In some embodiments, endogenous enzymes are replaced by enzymes with an alternate cofactor specificity from a different species.

Inventors:

Lo, Jonathan; Guss, Adam M.; van Dijken, Johannes Pieter; Shaw, IV, Arthur J.; Olson, Daniel G.; Herring, Christopher D.; Argyros, D. Aaron; Caiazza, Nicky

Issue Date:

2020-09-08

Research Org.:

Enchi Corp., Wellesley Hills, MA (United States); Dartmouth College, Hanover, NH (United States); UT-Battelle LLC/ORNL, Oak Ridge, TN (United States)

Sponsoring Org.:

USDOE Office of Science (SC)

OSTI Identifier:

1735231

Patent Number(s):

10767196

Application Number:

14/361,948

Assignee:

Enchi Corporation (Wellesley Hills, MA); Trustees of Dartmouth College (Hanover, NH); UT-Battelle, LLC (Oak Ridge, TN)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES

C12Y101/01001 - Alcohol dehydrogenase
C12Y101/01002 - Alcohol dehydrogenase

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/74 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
C12N9/0006 - {acting on CH-OH groups as donors

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION

Y02E50/10 - Biofuels

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P7/06 - Ethanol, i.e. non-beverage

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N9/0008 - {acting on the aldehyde or oxo group of donors

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DOE Contract Number:  

POS2-06ER64304; FC36-07GO17057

Resource Type:

Patent

Resource Relation:

Patent File Date: 11/30/2012

Country of Publication:

United States

Language:

English