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Title: RNA-guided transcriptional regulation

Abstract

Methods of modulating expression of a target nucleic acid in a cell are provided including introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to DNA, wherein the DNA includes the target nucleic acid, introducing into the cell a second foreign nucleic acid encoding a nuclease-null Cas9 protein that binds to the DNA and is guided by the one or more RNAs, introducing into the cell a third foreign nucleic acid encoding a transcriptional regulator protein or domain, wherein the one or more RNAs, the nuclease-null Cas9 protein, and the transcriptional regulator protein or domain are expressed, wherein the one or more RNAs, the nuclease-null Cas9 protein and the transcriptional regulator protein or domain co-localize to the DNA and wherein the transcriptional regulator protein or domain regulates expression of the target nucleic acid.

Inventors:
; ;
Issue Date:
Research Org.:
Harvard Univ., Cambridge, MA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1735230
Patent Number(s):
10767194
Application Number:
16/441,209
Assignee:
President and Fellows of Harvard College (Cambridge, MA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
DOE Contract Number:  
FG02-02ER63445
Resource Type:
Patent
Resource Relation:
Patent File Date: 06/14/2019
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Church, George M., Mali, Prashant G., and Esvelt, Kevin M. RNA-guided transcriptional regulation. United States: N. p., 2020. Web.
Church, George M., Mali, Prashant G., & Esvelt, Kevin M. RNA-guided transcriptional regulation. United States.
Church, George M., Mali, Prashant G., and Esvelt, Kevin M. Tue . "RNA-guided transcriptional regulation". United States. https://www.osti.gov/servlets/purl/1735230.
@article{osti_1735230,
title = {RNA-guided transcriptional regulation},
author = {Church, George M. and Mali, Prashant G. and Esvelt, Kevin M.},
abstractNote = {Methods of modulating expression of a target nucleic acid in a cell are provided including introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to DNA, wherein the DNA includes the target nucleic acid, introducing into the cell a second foreign nucleic acid encoding a nuclease-null Cas9 protein that binds to the DNA and is guided by the one or more RNAs, introducing into the cell a third foreign nucleic acid encoding a transcriptional regulator protein or domain, wherein the one or more RNAs, the nuclease-null Cas9 protein, and the transcriptional regulator protein or domain are expressed, wherein the one or more RNAs, the nuclease-null Cas9 protein and the transcriptional regulator protein or domain co-localize to the DNA and wherein the transcriptional regulator protein or domain regulates expression of the target nucleic acid.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2020},
month = {9}
}

Works referenced in this record:

Diverse CRISPRs Evolving in Human Microbiomes
journal, June 2012


Prokaryotic RNAi-Like System and Methods of Use
patent-application, August 2011


The VP16 transcription activation domain is functional when targeted to a promoter-proximal RNA sequence.
journal, November 1992


Target DNA interference with crRNA
patent-application, March 2010


Essential Features and Rational Design of CRISPR RNAs that Function with the Cas RAMP Module Complex to Cleave RNAs
journal, February 2012


Robust, synergistic regulation of human gene expression using TALE activators
journal, February 2013


Crisper-Cas Nickase Systems, Methods and Compositions for Sequence Manipulation in Eukaryotes
patent-application, October 2014


Synergistic and tunable human gene activation by combinations of synthetic transcription factors
journal, February 2013


CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes
journal, July 2013


Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression
journal, February 2013


Methods And Compositions For Rna-Directed Target Dna Modification And For Rna-Directed Modulation Of Transcription
patent-application, March 2014


Increasing Specificity for RNA-Guided Genome Editing
patent-application, January 2016


A CRISPR Approach to Gene Targeting
journal, September 2012


Crispr-based genome modification and regulation
patent-application, September 2014


RNA-Guided Human Genome Engineering via Cas9
journal, January 2013


Riboactivators: Transcription activation by noncoding RNA
journal, February 2009


CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering
journal, August 2013


Multiplex Genome Engineering Using CRISPR/Cas Systems
journal, January 2013


RNA-Guided Human Genome Engineering
patent-application, November 2014


Engineering of systems, methods and optimized guide compositions for sequence manipulation
patent-application, September 2015


The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli
journal, August 2011


Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system
journal, August 2013


Systems and methods for reading data from a storage medium
patent, February 2016


Methods of Generating Nucleic Acid Fragments
patent-application, September 2011


Endroibonuclease Compositions and Methods of Use Thereof
patent-application, May 2013


Optimized Crisper-Cas Double Nickase Systems, Methods and Compositions for Sequence Manipulation
patent-application, June 2016


A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity
journal, June 2012


Compositions and methods of nucleic acid-targeting nucleic acids
patent-application, October 2014


Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria
journal, September 2012


CRISPR/CAS Systems for Genomic Modification and Gene Modulation
patent-application, September 2014


RNA-guided genetic silencing systems in bacteria and archaea
journal, February 2012


Engineering of Systems, Methods and Optimized Guide Compositions for Sequence Manipulation
patent-application, November 2016


Evolution and classification of the CRISPR–Cas systems
journal, May 2011


Mature clustered, regularly interspaced, short palindromic repeats RNA (crRNA) length is measured by a ruler mechanism anchored at the precursor processing site
journal, December 2011


Methods and compositions for treating Huntington's disease
patent-application, September 2013


RNA-Guided Human Genome Engineering
patent-application, June 2016


Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA
journal, April 2014


Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity
journal, September 2013


RNA-guided transcriptional regulation
patent, February 2016


Clustered regularly interspaced short palindromic repeats (CRISPRs): the hallmark of an ingenious antiviral defense mechanism in prokaryotes
journal, July 2011


Crisper-Cas Nickase Systems, Methods and Compostions for Sequence Manipulations in Eukaryotes
patent-application, July 2017