Title: Precise microbiota engineering at the cellular level

Abstract

Stably immunized cells and methods of making stably immunized cells are provided. Methods of altering the microbiota of an ecological environment are provided. Methods of modifying target chromosomes are provided. Methods of delivering genetic material to target cells are provided.

Inventors:

Esvelt, Kevin M.; Yaung, Stephanie

Issue Date:

2020-08-25

Research Org.:

Harvard Univ., Cambridge, MA (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1735182

Patent Number(s):

10752906

Application Number:

15/034,682

Assignee:

President and Fellows of Harvard College (Cambridge, MA)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N2310/10 - Type of nucleic acid
C12N15/70 - Vectors or expression systems specially adapted for E. coli

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

A61K35/74 - Bacteria

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N2800/80 - Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
C12N15/902 - {using homologous recombination}
C12N15/1131 - {against viruses}
C12N1/38 - Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors
C12N1/20 - Bacteria {
C12N2310/20 - involving clustered regularly interspaced short palindromic repeats [CRISPRs]

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DOE Contract Number:  

FG02-02ER63445

Resource Type:

Patent

Resource Relation:

Patent File Date: 11/05/2014

Country of Publication:

United States

Language:

English