Title: Enzymes and methods for dealkylation of substrates

Abstract

Disclosed herein are enzymes and organisms useful for the dealkylation of products derived from lignin depolymerization, including the conversion of guaiacol or guaethol to catechol or the conversion of anisole to phenol. Methods of converting guaiacol or guaethol to catechol or anisole to phenol using enzymes or organisms expressing the same are also disclosed.

Inventors:

Johnson, Christopher W.; Beckham, Gregg T.

Issue Date:

2020-08-18

Research Org.:

National Renewable Energy Lab. (NREL), Golden, CO (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1735158

Patent Number(s):

10745719

Application Number:

16/211,816

Assignee:

Alliance for Sustainable Energy, LLC (Golden, CO)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

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C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES

C12Y118/01002 - Ferredoxin-NADP+ reductase

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P7/22 - aromatic

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES

C12Y114/00 - Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N9/0095 - {acting on iron-sulfur proteins as donor
C12N9/0071 - {acting on paired donors with incorporation of molecular oxygen

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DOE Contract Number:  

AC36-08GO28308

Resource Type:

Patent

Resource Relation:

Patent File Date: 12/06/2018

Country of Publication:

United States

Language:

English