Methods of designing programmable inducible promoters
Abstract
Described herein is a method for identifying synthetic inducible promoters that have specified induction and/or repression for DNA binding proteins such as an allosteric transcription factor and an inducer molecule. The method includes an in vitro selection from an unselected polynucleotide library comprising a plurality of random degeneracies, and an in vivo selection to produce an induced promoter library. Produced is an induction table, which allows the selection of a promoter with specific induction and/or repression properties. Also included are biosensors containing the synthetic inducible promoters.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of Wisconsin, Madison, WI (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1735154
- Patent Number(s):
- 10745693
- Application Number:
- 15/945,050
- Assignee:
- Wisconsin Alumni Research Foundation (Madison, WI)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- FC02-07ER64494
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 04/04/2018
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Raman, Srivatsan, Ansari, Aseem Zoe, Liu, Xiangyang, and Rodriguez-Martinez, Jose Arcadio. Methods of designing programmable inducible promoters. United States: N. p., 2020.
Web.
Raman, Srivatsan, Ansari, Aseem Zoe, Liu, Xiangyang, & Rodriguez-Martinez, Jose Arcadio. Methods of designing programmable inducible promoters. United States.
Raman, Srivatsan, Ansari, Aseem Zoe, Liu, Xiangyang, and Rodriguez-Martinez, Jose Arcadio. Tue .
"Methods of designing programmable inducible promoters". United States. https://www.osti.gov/servlets/purl/1735154.
@article{osti_1735154,
title = {Methods of designing programmable inducible promoters},
author = {Raman, Srivatsan and Ansari, Aseem Zoe and Liu, Xiangyang and Rodriguez-Martinez, Jose Arcadio},
abstractNote = {Described herein is a method for identifying synthetic inducible promoters that have specified induction and/or repression for DNA binding proteins such as an allosteric transcription factor and an inducer molecule. The method includes an in vitro selection from an unselected polynucleotide library comprising a plurality of random degeneracies, and an in vivo selection to produce an induced promoter library. Produced is an induction table, which allows the selection of a promoter with specific induction and/or repression properties. Also included are biosensors containing the synthetic inducible promoters.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Aug 18 00:00:00 EDT 2020},
month = {Tue Aug 18 00:00:00 EDT 2020}
}
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