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Title: Methods of designing programmable inducible promoters

Abstract

Described herein is a method for identifying synthetic inducible promoters that have specified induction and/or repression for DNA binding proteins such as an allosteric transcription factor and an inducer molecule. The method includes an in vitro selection from an unselected polynucleotide library comprising a plurality of random degeneracies, and an in vivo selection to produce an induced promoter library. Produced is an induction table, which allows the selection of a promoter with specific induction and/or repression properties. Also included are biosensors containing the synthetic inducible promoters.

Inventors:
; ; ;
Issue Date:
Research Org.:
Univ. of Wisconsin, Madison, WI (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1735154
Patent Number(s):
10745693
Application Number:
15/945,050
Assignee:
Wisconsin Alumni Research Foundation (Madison, WI)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
FC02-07ER64494
Resource Type:
Patent
Resource Relation:
Patent File Date: 04/04/2018
Country of Publication:
United States
Language:
English

Citation Formats

Raman, Srivatsan, Ansari, Aseem Zoe, Liu, Xiangyang, and Rodriguez-Martinez, Jose Arcadio. Methods of designing programmable inducible promoters. United States: N. p., 2020. Web.
Raman, Srivatsan, Ansari, Aseem Zoe, Liu, Xiangyang, & Rodriguez-Martinez, Jose Arcadio. Methods of designing programmable inducible promoters. United States.
Raman, Srivatsan, Ansari, Aseem Zoe, Liu, Xiangyang, and Rodriguez-Martinez, Jose Arcadio. Tue . "Methods of designing programmable inducible promoters". United States. https://www.osti.gov/servlets/purl/1735154.
@article{osti_1735154,
title = {Methods of designing programmable inducible promoters},
author = {Raman, Srivatsan and Ansari, Aseem Zoe and Liu, Xiangyang and Rodriguez-Martinez, Jose Arcadio},
abstractNote = {Described herein is a method for identifying synthetic inducible promoters that have specified induction and/or repression for DNA binding proteins such as an allosteric transcription factor and an inducer molecule. The method includes an in vitro selection from an unselected polynucleotide library comprising a plurality of random degeneracies, and an in vivo selection to produce an induced promoter library. Produced is an induction table, which allows the selection of a promoter with specific induction and/or repression properties. Also included are biosensors containing the synthetic inducible promoters.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2020},
month = {8}
}

Works referenced in this record:

T7 promoter-based expression system
patent, March 2003


Transcription factor-based biosensors for detecting dicarboxylic acids
patent, February 2014