Title: Production of itaconic acid and related molecules from aromatic compounds

Abstract

This disclosure provides a genetically-modified bacterium from the genus Pseudomonas that produces itaconate or trans-aconitate. The disclosure further provides methods for producing itaconate or trans-aconitate using a genetically-modified bacterium from the genus Pseudomonas.

Inventors:

Elmore, Joshua R.; Huenemann, Jay; Salvachua, Davinia; Beckham, Gregg T.; Guss, Adam M.

Issue Date:

2020-08-11

Research Org.:

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1735128

Patent Number(s):

10738333

Application Number:

16/397,256

Assignee:

UT-Battelle, LLC (Oak Ridge, TN); Alliance for Sustainable Energy, LLC (Golden, CO)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N9/90 - Isomerases

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K14/375 - from Basidiomycetes

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P7/48 - Tricarboxylic acids, e.g. citric acid
C12P7/46 - Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES

C12Y401/01006 - Aconitate decarboxylase

C - CHEMISTRY C12 - BIOCHEMISTRY C12R - PROCESSES USING MICROORGANISMS

C12R1/38 - Pseudomonas

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K14/32 - from Bacillus

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/74 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
C12N9/88 - Lyases

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P7/44 - Polycarboxylic acids

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES

C12Y203/03001 - Citrate
C12Y503/03007 - Aconitate DELTA-isomerase

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N1/20 - Bacteria {
C12N9/1025 - {Acyltransferases

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DOE Contract Number:  

AC05-00OR22725; AC36-08GO28308

Resource Type:

Patent

Resource Relation:

Patent File Date: 04/29/2019

Country of Publication:

United States

Language:

English