Title: Biomolecular patterning of three dimensional tissue scaffolds

Abstract

The invention provides methods and compositions for making and using collagen-glycosaminoglycan three-dimensional scaffolds immobilized with biomolecules that are spatially and temporally patterned. The method comprises adding benzophenone to a collagen-glycosaminoglycan three dimensional scaffold in the dark; adding one or more biomolecules to one or more areas of the collagen-glycosaminoglycan three-dimensional scaffold (which can be done optionally in the dark or in the light); and exposing the collagen-2glycosaminoglycan three-dimensional scaffold to light at a wavelength of about 350 to about 365 nm.

Inventors:

Bailey, Ryan C.; Harley, Brendan A.; Martin, Teresa A.; Caliari, Steven R.

Issue Date:

2020-08-11

Research Org.:

Univ. of Illinois at Urbana-Champaign, IL (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1735120

Patent Number(s):

10736992

Application Number:

14/001,327

Assignee:

The Board of Trustees of the University of Illinois (Urbana, IL)

Patent Classifications (CPCs):

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61L - METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL

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A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

A61K35/12 - Materials from mammals
A61K35/32 - Bones
A61K38/1858 - {Platelet-derived growth factor [PDGF]}
A61K38/30 - Insulin-like growth factors

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61L - METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL

A61L2430/02 - for reconstruction of bones
A61L2430/06 - for cartilage reconstruction, e.g. meniscus
A61L2430/20 - for reconstruction of the heart, e.g. heart valves
A61L2430/28 - for liver reconstruction
A61L2430/30 - for muscle reconstruction
A61L2430/32 - for nerve reconstruction
A61L2430/40 - Preparation and treatment of biological tissue for implantation, e.g. decellularisation, cross-linking
A61L27/26 - Mixtures of macromolecular compounds
A61L27/38 - {containing added animal cells
A61L27/48 - with macromolecular fillers
A61L27/50 - Materials characterised by their function or physical properties {, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials}
A61L27/54 - Biologically active materials, e.g. therapeutic substances {
A61L27/56 - Porous materials, {e.g. foams or sponges}

C - CHEMISTRY C08 - ORGANIC MACROMOLECULAR COMPOUNDS C08B - POLYSACCHARIDES

C08B37/0063 - {Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate

C - CHEMISTRY C08 - ORGANIC MACROMOLECULAR COMPOUNDS C08L - COMPOSITIONS OF MACROMOLECULAR COMPOUNDS

C08L89/06 - derived from leather or skin {, e.g. gelatin}

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N2533/54 - Collagen
C12N5/0062 - {General methods for three-dimensional culture}
C12N5/0654 - {Osteocytes, Osteoblasts, Odontocytes
C12N5/0655 - {Chondrocytes
C12N5/0657 - {Cardiomyocytes
C12N5/0658 - {Skeletal muscle cells, e.g. myocytes, myotubes, myoblasts}

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DOE Contract Number:  

FG02-07ER46453; FG02-07ER46471

Resource Type:

Patent

Resource Relation:

Patent File Date: 02/27/2012

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES