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Title: Chromatin activity precipitation method and system

Abstract

Methods and systems for identifying binding sites in macromolecules using small molecule mimics of naturally occurring molecules is disclosed. A reactive probe is provided that mimics small molecule cofactors. A target macromolecule is irreversibly bound to the probe in vivo to selectively pull down or precipitate probe-bound macromolecules. The macromolecules may be, but are not limited to, DNA, RNA, and proteins.

Inventors:
;
Issue Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1735099
Patent Number(s):
10731202
Application Number:
15/527,996
Assignee:
Battelle Memorial Institute (Richland, WA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Patent
Resource Relation:
Patent File Date: 11/11/2015
Country of Publication:
United States
Language:
English

Citation Formats

Lindemann, Stephen R., and Wright, Aaron T. Chromatin activity precipitation method and system. United States: N. p., 2020. Web.
Lindemann, Stephen R., & Wright, Aaron T. Chromatin activity precipitation method and system. United States.
Lindemann, Stephen R., and Wright, Aaron T. Tue . "Chromatin activity precipitation method and system". United States. https://www.osti.gov/servlets/purl/1735099.
@article{osti_1735099,
title = {Chromatin activity precipitation method and system},
author = {Lindemann, Stephen R. and Wright, Aaron T.},
abstractNote = {Methods and systems for identifying binding sites in macromolecules using small molecule mimics of naturally occurring molecules is disclosed. A reactive probe is provided that mimics small molecule cofactors. A target macromolecule is irreversibly bound to the probe in vivo to selectively pull down or precipitate probe-bound macromolecules. The macromolecules may be, but are not limited to, DNA, RNA, and proteins.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2020},
month = {8}
}

Works referenced in this record:

Fully Functionalized Small-Molecule Probes for Integrated Phenotypic Screening and Target Identification
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Identification of Widespread Adenosine Nucleotide Binding in Mycobacterium tuberculosis
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A cleavable azide resin for direct click chemistry mediated enrichment of alkyne-labeled proteins
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Mechanism-Based Profiling of Enzyme Families
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A complex task? Direct modulation of transcription factors with small molecules
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