Title: Droplet array for single-cell analysis

Abstract

Techniques, systems, and devices are described for implementing a droplet array for single-cell analysis. A method of conducting single-cell analysis comprises generating a plurality of droplets, wherein each of the plurality of droplets contains a core material surrounded by a protective shell; loading the plurality of droplets, via a carrying fluid, onto an array including a plurality of trap structures, wherein the plurality of droplets are held by the plurality of trap structures; selecting a target droplet, held by a trap structure, from the plurality of droplets; and releasing the target droplet from the trap structure.

Inventors:

Simon, Melinda; Beer, N. Reginald

Issue Date:

2020-07-21

Research Org.:

Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1735045

Patent Number(s):

10718004

Application Number:

15/385,799

Assignee:

Lawrence Livermore National Security, LLC (Livermore, CA)

Patent Classifications (CPCs):

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

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B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

B01L2200/0652 - Sorting or classification of particles or molecules
B01L2200/0668 - Trapping microscopic beads
B01L2300/0816 - Cards, e.g. flat sample carriers usually with flow in two horizontal directions
B01L2300/0851 - Bottom walls
B01L2400/086 - using baffles or other fixed flow obstructions
B01L3/502761 - {specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
B01L3/502784 - {specially adapted for droplet or plug flow, e.g. digital microfluidics

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q1/24 - Methods of sampling, or inoculating or spreading a sample

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DOE Contract Number:  

AC52-07NA27344

Resource Type:

Patent

Resource Relation:

Patent File Date: 12/20/2016

Country of Publication:

United States

Language:

English

Subject:

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES