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Title: Method using CO for extending the useful shelf-life of refrigerated red blood cells

A method is disclosed using CO for extending the useful shelf-life of refrigerated red blood cells. Carbon monoxide is utilized for stabilizing hemoglobin in red blood cells to be stored at low temperature. Changes observed in the stored cells are similar to those found in normal red cell aging in the body, the extent thereof being directly related to the duration of refrigerated storage. Changes in cell buoyant density, vesiculation, and the tendency of stored cells to bind autologous IgG antibody directed against polymerized band 3 IgG, all of which are related to red blood cell senescence and increase with refrigerated storage time, have been substantially slowed when red blood cells are treated with CO. Removal of the carbon monoxide from the red blood cells is readily and efficiently accomplished by photolysis in the presence of oxygen so that the stored red blood cells may be safely transfused into a recipient. 5 figs.
Inventors:
Issue Date:
OSTI Identifier:
170468
Assignee:
Univ. of California, Los Alamos, NM (United States) PTO; SCA: 550600; PA: EDB-96:030493; SN: 96001517617
Patent Number(s):
US 5,476,764/A/
Application Number:
PAN: 8-308,028
Contract Number:
W-7405-ENG-36
Resource Relation:
Other Information: PBD: 19 Dec 1995
Research Org:
University of California
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; ERYTHROCYTES; STORAGE LIFE; HEMOGLOBIN; STABILIZATION; CARBON MONOXIDE; CHEMICAL REACTIONS; TRANSFUSIONS