skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: DNA purification by triplex-affinity capture and affinity capture electrophoresis

Abstract

The invention provides a method for purifying or isolating double stranded DNA intact using triple helix formation. The method includes the steps of complexing an oligonucleotide and double stranded DNA to generate a triple helix and immobilization of the triple helix on a solid phase by means of a molecular recognition system such as avidin/biotin. The purified DNA is then recovered intact by treating the solid phase with a reagent that breaks the bonds between the oligonucleotide and the intact double stranded DNA while not affecting the Watson-Crick base pairs of the double helix. The present invention also provides a method for purifying or isolating double stranded DNA intact by complexing the double stranded DNA with a specific binding partner and recovering the complex during electrophoresis by immobilizing it on a solid phase trap imbedded in an electrophoretic gel. 6 figs.

Inventors:
; ;
Issue Date:
Research Org.:
University of California
OSTI Identifier:
170464
Patent Number(s):
5,482,836
Application Number:
PAN: 8-004,552; CNN: Grant CA39782
Assignee:
Univ. of California, Oakland, CA (United States)
DOE Contract Number:  
AC03-76SF00098
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 9 Jan 1996
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA; ELECTRON CAPTURE; FRACTIONATION; HELICAL CONFIGURATION; MOLECULAR STRUCTURE; CHEMICAL BONDS; MOLECULAR BIOLOGY

Citation Formats

Cantor, C R, Ito, Takashi, and Smith, C L. DNA purification by triplex-affinity capture and affinity capture electrophoresis. United States: N. p., 1996. Web.
Cantor, C R, Ito, Takashi, & Smith, C L. DNA purification by triplex-affinity capture and affinity capture electrophoresis. United States.
Cantor, C R, Ito, Takashi, and Smith, C L. Tue . "DNA purification by triplex-affinity capture and affinity capture electrophoresis". United States.
@article{osti_170464,
title = {DNA purification by triplex-affinity capture and affinity capture electrophoresis},
author = {Cantor, C R and Ito, Takashi and Smith, C L},
abstractNote = {The invention provides a method for purifying or isolating double stranded DNA intact using triple helix formation. The method includes the steps of complexing an oligonucleotide and double stranded DNA to generate a triple helix and immobilization of the triple helix on a solid phase by means of a molecular recognition system such as avidin/biotin. The purified DNA is then recovered intact by treating the solid phase with a reagent that breaks the bonds between the oligonucleotide and the intact double stranded DNA while not affecting the Watson-Crick base pairs of the double helix. The present invention also provides a method for purifying or isolating double stranded DNA intact by complexing the double stranded DNA with a specific binding partner and recovering the complex during electrophoresis by immobilizing it on a solid phase trap imbedded in an electrophoretic gel. 6 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1996},
month = {1}
}