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Title: DNA purification by triplex-affinity capture and affinity capture electrophoresis

The invention provides a method for purifying or isolating double stranded DNA intact using triple helix formation. The method includes the steps of complexing an oligonucleotide and double stranded DNA to generate a triple helix and immobilization of the triple helix on a solid phase by means of a molecular recognition system such as avidin/biotin. The purified DNA is then recovered intact by treating the solid phase with a reagent that breaks the bonds between the oligonucleotide and the intact double stranded DNA while not affecting the Watson-Crick base pairs of the double helix. The present invention also provides a method for purifying or isolating double stranded DNA intact by complexing the double stranded DNA with a specific binding partner and recovering the complex during electrophoresis by immobilizing it on a solid phase trap imbedded in an electrophoretic gel. 6 figs.
Inventors:
; ;
Issue Date:
OSTI Identifier:
170464
Assignee:
Univ. of California, Oakland, CA (United States) PTO; SCA: 550200; PA: EDB-96:030478; SN: 96001517581
Patent Number(s):
US 5,482,836/A/
Application Number:
PAN: 8-004,552; CNN: Grant CA39782
Contract Number:
AC03-76SF00098
Resource Relation:
Other Information: PBD: 9 Jan 1996
Research Org:
University of California
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA; ELECTRON CAPTURE; FRACTIONATION; HELICAL CONFIGURATION; MOLECULAR STRUCTURE; CHEMICAL BONDS; MOLECULAR BIOLOGY